LOCUS       V035550                10027 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035550
VERSION     V035550
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4053
                     /label="YTHDF2(NM_016258)"
                     /note="YTHDF2(NM_016258)"
                     /gene="YTHDF2"
     CDS             4060..4125
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4169..4380
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4393..4661
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4690..4699
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4720..5382
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5452..5505
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5506..6105
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6106..6694
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6768..7001
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7073..7194
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7234..7369
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7402..7418)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7426..7442
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7450..7480)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7495..7516
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7804..8392)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8563..9423)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9424..9528)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10002..10018
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cggccagcag cctcttggag
     2341 cagagaccaa aaggtcaagg aaacaaagta caaaatggat ctgtacatca aaaggatgga
     2401 ttaaacgatg atgattttga accttacttg agtccacagg caaggcccaa taatgcatat
     2461 actgccatgt cagattccta cttacccagt tactacagtc cctccattgg cttctcctat
     2521 tctttgggtg aagctgcttg gtctacgggg ggtgacacag ccatgcccta cttaacttct
     2581 tatggacagc tgagcaacgg agagccccac ttcctaccag atgcaatgtt tgggcaacca
     2641 ggagccctag gtagcactcc atttcttggt cagcatggtt ttaatttctt tcccagtggg
     2701 attgacttct cagcatgggg aaataacagt tctcagggac agtctactca gagctctgga
     2761 tatagtagca attatgctta tgcacctagc tccttaggtg gagccatgat tgatggacag
     2821 tcagcttttg ccaatgagac cctcaataag gctcctggca tgaatactat agaccaaggg
     2881 atggcagcac tgaagttggg tagcacagaa gttgcaagca atgttccaaa agttgtaggt
     2941 tctgctgttg gtagcgggtc cattactagt aacatcgtgg cttccaatag tttgcctcca
     3001 gccaccattg ctcctccaaa accagcatct tgggctgata ttgctagcaa gcctgcaaaa
     3061 cagcaaccta aactgaagac caagaatggc attgcagggt caagtcttcc gccacccccg
     3121 ataaagcata acatggatat tggaacttgg gataacaagg gtcccgttgc aaaagccccc
     3181 tcacaggctt tggttcagaa tataggtcag ccaacccagg ggtctcctca gcctgtaggt
     3241 cagcaggcta acaatagccc accagtggct caggcatcag tagggcaaca gacacagcca
     3301 ttgcctccac ctccaccaca gcctgcccag ctttcagtcc agcaacaggc agctcagcca
     3361 acccgctggg tagcacctcg gaaccgtggc agtgggttcg gtcataatgg ggtggatggt
     3421 aatggagtag gacagtctca ggctggttct ggatctactc cttcagaacc ccacccagtg
     3481 ttggagaagc ttcggtccat taataactat aaccccaaag attttgactg gaatctgaaa
     3541 catggccggg ttttcatcat taagagctac tctgaggacg atattcaccg ttccattaag
     3601 tataatattt ggtgcagcac agagcatggt aacaagagac tggatgctgc ttatcgttcc
     3661 atgaacggga aaggccccgt ttacttactt ttcagtgtca acggcagtgg acacttctgt
     3721 ggcgtggcag aaatgaaatc tgctgtggac tacaacacat gtgcaggtgt gtggtcccag
     3781 gacaaatgga agggtcgttt tgatgtcagg tggatttttg tgaaggacgt tcccaatagc
     3841 caactgcgac acattcgcct agagaacaac gagaataaac cagtgaccaa ctctagggac
     3901 actcaggaag tgcctctgga aaaggctaag caggtgttga aaattatagc cagctacaag
     3961 cacaccactt ccatttttga tgacttctca cactatgaga aacgccaaga ggaagaagaa
     4021 agtgttaaaa aggaacgtca aggtcgtggg aaaggatccg actacaaaga ccatgacggt
     4081 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4141 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4201 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4261 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4321 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4381 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4441 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4501 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4561 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     4621 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     4681 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     4741 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     4801 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     4861 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     4921 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     4981 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5041 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5101 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5161 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5221 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5281 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5341 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5401 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5461 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5521 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5581 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5641 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5701 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5761 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5821 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5881 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5941 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6001 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6061 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6121 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6181 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6241 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6301 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6361 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6421 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6481 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6541 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6601 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6661 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     6721 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6781 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     6841 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6901 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6961 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7021 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7201 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7321 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7381 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8221 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9541 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9601 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9781 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     9841 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9901 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     9961 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10021 caagctg
//
