LOCUS       V038546                 9954 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038546
VERSION     V038546
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..3973
                     /label="YTHDF1(NM_017798)"
                     /note="YTHDF1(NM_017798)"
                     /gene="YTHDF1"
     CDS             3980..4069
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        4113..4324
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4337..4605
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4634..4643
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4664..5326
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5396..5449
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5450..6049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6050..6637
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6711..6944
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7016..7137
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7156..7291
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7329..7345)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7353..7369
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7377..7407)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7422..7443
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7731..8319)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8490..9350)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9351..9455)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9929..9945
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt cggccaccag cgtggacacc cagagaacaa aaggacaaga
     2341 taataaagta caaaatggtt cgttacatca gaaggataca gttcatgaca atgactttga
     2401 gccctacctt actggacagt caaatcagag taacagttac ccctcaatga gcgaccccta
     2461 cctgtccagc tattacccgc cgtccattgg atttccttac tccctcaatg aggctccgtg
     2521 gtctactgca ggggaccctc cgattccata cctcaccacc tacggacagc tcagtaacgg
     2581 agaccatcat tttatgcacg atgctgtttt tgggcagcct gggggcctgg ggaacaacat
     2641 ctatcagcac aggttcaatt ttttccctga aaaccctgcg ttctcagcat gggggacaag
     2701 tgggtctcaa ggtcagcaga cccagagctc cgcgtatggg agcagctaca cctacccccc
     2761 gagctccctg ggtggcacgg tggttgatgg gcagccaggc tttcacagcg acaccctcag
     2821 caaggccccc gggatgaaca gcctggagca gggcatggtt ggcctgaaga ttggggacgt
     2881 cagctcctcc gccgtcaaga cggtgggctc tgtcgtcagc agcgtggcac tgactggtgt
     2941 cctttctggc aacggtggga caaatgtgaa catgccagtt tcaaagccga cctcgtgggc
     3001 tgccattgcc agcaagcctg caaaaccaca gcctaaaatg aaaacaaaga gcgggcctgt
     3061 catggggggt gggctgcccc ctccacccat aaagcataac atggacattg gcacctggga
     3121 taacaagggg cctgtgccga aggccccagt cccccagcag gcaccctctc cacaggctgc
     3181 cccacagccc cagcaggtgg ctcagcctct cccagcacag cccccagctt tggctcaacc
     3241 gcagtatcag agccctcagc agccacccca gacccgctgg gttgccccac gcaacagaaa
     3301 cgcggcgttt gggcagagcg gaggggctgg cagcgatagc aactctcctg gaaacgtcca
     3361 gcctaattct gcccccagcg tcgaatccca ccccgtcctt gaaaaactga aggctgctca
     3421 cagctacaac ccgaaagagt ttgagtggaa tctgaaaagc gggcgtgtgt tcatcatcaa
     3481 gagctactct gaggacgaca tccaccgctc cattaagtac tccatctggt gtagcacaga
     3541 gcacggcaac aagcgcctgg acagcgcctt ccgctgcatg agcagcaagg ggcccgtcta
     3601 cctgctcttc agcgtcaatg ggagtgggca tttttgtggg gtggccgaga tgaagtcccc
     3661 cgtggactac ggcaccagtg ccggggtctg gtctcaggac aagtggaagg ggaagtttga
     3721 tgtccagtgg atttttgtta aggatgtacc caataaccag ctccggcaca tcaggctgga
     3781 gaataacgac aacaaaccgg tcacaaactc ccgggacacc caggaggtgc ccttagaaaa
     3841 agccaagcaa gtgctgaaaa ttatcagttc ctacaagcac acaacctcca tcttcgacga
     3901 ctttgctcac tacgagaagc gccaggagga ggaggaggtg gtgcgcaagg aacggcagag
     3961 tcgaaacaaa caaggatccg aacaaaaact aatatcagag gaagatttgg aacagaaact
     4021 aatcagtgaa gaagatttag agcaaaagtt aatttccgag gaggacttat agcggccgcg
     4081 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4141 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     4201 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4261 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4321 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     4381 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     4441 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     4501 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     4561 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     4621 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     4681 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     4741 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
     4801 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     4861 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     4921 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
     4981 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
     5041 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     5101 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
     5161 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     5221 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
     5281 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
     5341 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg
     5401 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta
     5461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5641 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5701 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5881 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct
     6061 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6121 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6181 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6241 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6301 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6361 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6421 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     6481 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6541 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     6601 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac
     6661 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6721 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     6781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6961 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7141 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7261 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     7321 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7381 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7441 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7501 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7561 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7621 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7681 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7741 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7801 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7861 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7921 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7981 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8041 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8101 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8161 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8221 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8281 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8341 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8401 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8461 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8521 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8581 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8641 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8701 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8761 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8821 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8881 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8941 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     9001 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9061 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9121 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9181 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9241 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9301 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9361 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9421 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9481 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9541 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     9601 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9661 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9721 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9781 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     9841 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9901 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
