LOCUS V022597 10450 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022597 VERSION V022597 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2297..4492 /label="XRCC5(NM_021141)" /note="XRCC5(NM_021141)" /gene="XRCC5" CDS 4499..4564 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4609..4820 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4833..5101 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 5130..5139 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 5160..5822 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5892..5945 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5946..6545 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6546..7133 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 7207..7440 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7512..7633 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7652..7787 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7825..7841) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7849..7865 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7873..7903) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7918..7939 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8227..8815) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8986..9846) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9847..9951) /gene="bla" /label="AmpR promoter" primer_bind 10425..10441 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgg tgcggtcggg gaataaggca gctgttgtgc tgtgtatgga 2341 cgtgggcttt accatgagta actccattcc tggtatagaa tccccatttg aacaagcaaa 2401 gaaggtgata accatgtttg tacagcgaca ggtgtttgct gagaacaagg atgagattgc 2461 tttagtcctg tttggtacag atggcactga caatcccctt tctggtgggg atcagtatca 2521 gaacatcaca gtgcacagac atctgatgct accagatttt gatttgctgg aggacattga 2581 aagcaaaatc caaccaggtt ctcaacaggc tgacttcctg gatgcactaa tcgtgagcat 2641 ggatgtgatt caacatgaaa caataggaaa gaagtttgag aagaggcata ttgaaatatt 2701 cactgacctc agcagccgat tcagcaaaag tcagctggat attataattc atagcttgaa 2761 gaaatgtgac atctccctgc aattcttctt gcctttctca cttggcaagg aagatggaag 2821 tggggacaga ggagatggcc cctttcgctt aggtggccat gggccttcct ttccactaaa 2881 aggaattacc gaacagcaaa aagaaggtct tgagatagtg aaaatggtga tgatatcttt 2941 agaaggtgaa gatgggttgg atgaaattta ttcattcagt gagagtctga gaaaactgtg 3001 cgtcttcaag aaaattgaga ggcattccat tcactggccc tgccgactga ccattggctc 3061 caatttgtct ataaggattg cagcctataa atcgattcta caggagagag ttaaaaagac 3121 ttggacagtt gtggatgcaa aaaccctaaa aaaagaagat atacaaaaag aaacagttta 3181 ttgcttaaat gatgatgatg aaactgaagt tttaaaagag gatattattc aagggttccg 3241 ctatggaagt gatatagttc ctttctctaa agtggatgag gaacaaatga aatataaatc 3301 ggaggggaag tgcttctctg ttttgggatt ttgtaaatct tctcaggttc agagaagatt 3361 cttcatggga aatcaagttc taaaggtctt tgcagcaaga gatgatgagg cagctgcagt 3421 tgcactttcc tccctgattc atgctttgga tgacttagac atggtggcca tagttcgata 3481 tgcttatgac aaaagagcta atcctcaagt cggcgtggct tttcctcata tcaagcataa 3541 ctatgagtgt ttagtgtatg tgcagctgcc tttcatggaa gacttgcggc aatacatgtt 3601 ttcatccttg aaaaacagta agaaatatgc tcccaccgag gcacagttga atgctgttga 3661 tgctttgatt gactccatga gcttggcaaa gaaagatgag aagacagaca cccttgaaga 3721 cttgtttcca accaccaaaa tcccaaatcc tcgatttcag agattatttc agtgtctgct 3781 gcacagagct ttacatcccc gggagcctct acccccaatt cagcagcata tttggaatat 3841 gctgaatcct cccgctgagg tgacaacaaa aagtcagatt cctctctcta aaataaagac 3901 cctttttcct ctgattgaag ccaagaaaaa ggatcaagtg actgctcagg aaattttcca 3961 agacaaccat gaagatggac ctacagctaa aaaattaaag actgagcaag ggggagccca 4021 cttcagcgtc tccagtctgg ctgaaggcag tgtcacctct gttggaagtg tgaatcctgc 4081 tgaaaacttc cgtgttctag tgaaacagaa gaaggccagc tttgaggaag cgagtaacca 4141 gctcataaat cacatcgaac agtttttgga tactaatgaa acaccgtatt ttatgaagag 4201 catagactgc atccgagcct tccgggaaga agccattaag ttttcagaag agcagcgctt 4261 taacaacttc ctgaaagccc ttcaagagaa agtggaaatt aaacaattaa atcatttctg 4321 ggaaattgtt gtccaggatg gaattactct gatcaccaaa gaggaagcct ctggaagttc 4381 tgtcacagct gaggaagcca aaaagtttct ggcccccaaa gacaaaccaa gtggagacac 4441 agcagctgta tttgaagaag gtggtgatgt ggacgattta ttggacatga taggatccga 4501 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga 4561 caagtgagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca 4621 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga 4681 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 4741 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 4801 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc 4861 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct 4921 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg 4981 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc 5041 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat 5101 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc 5161 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg 5221 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca 5281 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc 5341 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg 5401 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca 5461 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc 5521 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc 5581 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg 5641 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc 5701 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca 5761 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg 5821 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca 5881 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg 5941 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 6001 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 6061 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 6121 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 6181 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 6241 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 6301 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 6361 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 6421 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 6481 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 6541 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 6601 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 6661 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 6721 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 6781 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 6841 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 6901 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 6961 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 7021 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 7081 gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac 7141 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 7201 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 7261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 7321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 7381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 7441 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 7501 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 7561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7621 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 7681 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7741 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 7801 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7861 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7921 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7981 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 8041 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 8101 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 8161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8641 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 8701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 9001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 9061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 9121 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9841 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9901 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9961 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 10021 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 10081 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 10141 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 10201 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 10261 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 10321 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 10381 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 10441 tgccaagctg //