LOCUS       V022597                10450 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022597
VERSION     V022597
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4492
                     /label="XRCC5(NM_021141)"
                     /note="XRCC5(NM_021141)"
                     /gene="XRCC5"
     CDS             4499..4564
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4609..4820
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4833..5101
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5130..5139
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5160..5822
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5892..5945
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5946..6545
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6546..7133
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7207..7440
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7512..7633
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7652..7787
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7825..7841)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7849..7865
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7873..7903)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7918..7939
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8227..8815)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8986..9846)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9847..9951)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10425..10441
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg tgcggtcggg gaataaggca gctgttgtgc tgtgtatgga
     2341 cgtgggcttt accatgagta actccattcc tggtatagaa tccccatttg aacaagcaaa
     2401 gaaggtgata accatgtttg tacagcgaca ggtgtttgct gagaacaagg atgagattgc
     2461 tttagtcctg tttggtacag atggcactga caatcccctt tctggtgggg atcagtatca
     2521 gaacatcaca gtgcacagac atctgatgct accagatttt gatttgctgg aggacattga
     2581 aagcaaaatc caaccaggtt ctcaacaggc tgacttcctg gatgcactaa tcgtgagcat
     2641 ggatgtgatt caacatgaaa caataggaaa gaagtttgag aagaggcata ttgaaatatt
     2701 cactgacctc agcagccgat tcagcaaaag tcagctggat attataattc atagcttgaa
     2761 gaaatgtgac atctccctgc aattcttctt gcctttctca cttggcaagg aagatggaag
     2821 tggggacaga ggagatggcc cctttcgctt aggtggccat gggccttcct ttccactaaa
     2881 aggaattacc gaacagcaaa aagaaggtct tgagatagtg aaaatggtga tgatatcttt
     2941 agaaggtgaa gatgggttgg atgaaattta ttcattcagt gagagtctga gaaaactgtg
     3001 cgtcttcaag aaaattgaga ggcattccat tcactggccc tgccgactga ccattggctc
     3061 caatttgtct ataaggattg cagcctataa atcgattcta caggagagag ttaaaaagac
     3121 ttggacagtt gtggatgcaa aaaccctaaa aaaagaagat atacaaaaag aaacagttta
     3181 ttgcttaaat gatgatgatg aaactgaagt tttaaaagag gatattattc aagggttccg
     3241 ctatggaagt gatatagttc ctttctctaa agtggatgag gaacaaatga aatataaatc
     3301 ggaggggaag tgcttctctg ttttgggatt ttgtaaatct tctcaggttc agagaagatt
     3361 cttcatggga aatcaagttc taaaggtctt tgcagcaaga gatgatgagg cagctgcagt
     3421 tgcactttcc tccctgattc atgctttgga tgacttagac atggtggcca tagttcgata
     3481 tgcttatgac aaaagagcta atcctcaagt cggcgtggct tttcctcata tcaagcataa
     3541 ctatgagtgt ttagtgtatg tgcagctgcc tttcatggaa gacttgcggc aatacatgtt
     3601 ttcatccttg aaaaacagta agaaatatgc tcccaccgag gcacagttga atgctgttga
     3661 tgctttgatt gactccatga gcttggcaaa gaaagatgag aagacagaca cccttgaaga
     3721 cttgtttcca accaccaaaa tcccaaatcc tcgatttcag agattatttc agtgtctgct
     3781 gcacagagct ttacatcccc gggagcctct acccccaatt cagcagcata tttggaatat
     3841 gctgaatcct cccgctgagg tgacaacaaa aagtcagatt cctctctcta aaataaagac
     3901 cctttttcct ctgattgaag ccaagaaaaa ggatcaagtg actgctcagg aaattttcca
     3961 agacaaccat gaagatggac ctacagctaa aaaattaaag actgagcaag ggggagccca
     4021 cttcagcgtc tccagtctgg ctgaaggcag tgtcacctct gttggaagtg tgaatcctgc
     4081 tgaaaacttc cgtgttctag tgaaacagaa gaaggccagc tttgaggaag cgagtaacca
     4141 gctcataaat cacatcgaac agtttttgga tactaatgaa acaccgtatt ttatgaagag
     4201 catagactgc atccgagcct tccgggaaga agccattaag ttttcagaag agcagcgctt
     4261 taacaacttc ctgaaagccc ttcaagagaa agtggaaatt aaacaattaa atcatttctg
     4321 ggaaattgtt gtccaggatg gaattactct gatcaccaaa gaggaagcct ctggaagttc
     4381 tgtcacagct gaggaagcca aaaagtttct ggcccccaaa gacaaaccaa gtggagacac
     4441 agcagctgta tttgaagaag gtggtgatgt ggacgattta ttggacatga taggatccga
     4501 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     4561 caagtgagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     4621 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     4681 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4741 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4801 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     4861 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     4921 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     4981 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5041 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     5101 ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc
     5161 ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg
     5221 tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca
     5281 aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc
     5341 acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg
     5401 gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca
     5461 gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc
     5521 ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc
     5581 tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg
     5641 acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc
     5701 ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca
     5761 gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg
     5821 ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca
     5881 ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     5941 gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     6001 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     6061 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6121 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6181 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6241 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6301 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6361 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6421 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6481 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6541 cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6601 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6661 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6721 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6781 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6841 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6901 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6961 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7021 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7081 gtctccgcct tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac
     7141 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7201 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
     7261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7441 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7501 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7621 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
     7681 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7741 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
     7801 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7861 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7921 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7981 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8041 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8101 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8641 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     8701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9121 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9841 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9901 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9961 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10021 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10081 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    10141 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    10201 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10261 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    10321 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10381 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10441 tgccaagctg
//
