LOCUS V034138 11424 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034138 VERSION V034138 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" regulatory 2279..2288 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 2288..2311 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 2312..3814 /label="Wasl(NM_028459)" /note="Wasl(NM_028459)" /gene="Wasl" CDS 3815..3871 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from porcine teschovirus-1 polyprotein" /transl_table=1 /translation="ATNFSLLKQAGDVEENPGP" /label="P2A" CDS 3884..5536 /EC_number="" /codon_start=1 /experiment="" /gene="luc" /product="firefly luciferase" /protein_id="" /transl_table=1 /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL*" /label="luciferase" promoter 5583..5794 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 5807..6075 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 6104..6113 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 6134..6796 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 6866..6919 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 6920..7519 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7520..8107 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8181..8414 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8486..8607 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8626..8761 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(8799..8815) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 8823..8839 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(8847..8877) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 8892..8913 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9201..9789) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(9960..10820) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(10821..10925) /gene="bla" /label="AmpR promoter" primer_bind 11399..11415 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatggat tacaaggatg acgacgataa gatgagctcg ggccagcagc ccccgcggag 2341 ggtcaccaac gtgggctccc tgctgctcac cccgcaagag aacgagtctc tcttctcctt 2401 cctcggcaag aaatgtgtga ctatgtcttc agcagtggtg cagttgtatg cagcagatcg 2461 gaactgtatg tgggcaaaga agtgcagtgg tgtcgcttgt ctggttaagg acaatcctca 2521 gagatcttat tttttaagaa tatttgacat taaggatggg aaattactgt gggaacaaga 2581 gctatacaat aactttgtat ataatagtcc tagaggatat tttcatacct ttgctggaga 2641 tacttgtcaa gtagctctta attttgccaa tgaagaagaa gcaaaaaagt tccgaaaagc 2701 agttacagac ctgttgggcc gacgacaaag gaaatctgaa aaaagacgag atgctccaaa 2761 tggtcccaat ctacccatgg ctacagttga cataaaaaat ccagaaatca caacaaatag 2821 attttatggt tcacaagtca acaacatctc ccacaccaaa gaaaagaaga aaggaaaagc 2881 taaaaagaag agattaacca aggcagatat tggaacacca agtaatttcc agcacattgg 2941 gcatgttgga tgggatccaa atacaggttt tgatctaaat aatttggatc cagaattaaa 3001 gaatcttttt gatatgtgtg ggatctctga ggcccagctt aaagacagag aaacatcaaa 3061 agttatttat gactttattg aaaaaacagg aggtgtagaa gctgttaaaa acgaactccg 3121 aagacaagca ccaccaccac ctccaccctc gagaggagga cctccccctc ctcctccccc 3181 tcctcatagc tcaggtcctc ctccccctcc tgcccgagga aggggggctc ctcctccccc 3241 accttcaaga gctcctactg ctgcacctcc acctccacct ccttctaggc ctggtgttgt 3301 tgttcctccg ccccctccaa acaggatgta ccctcctcca ccaccagcgc tgccctcttc 3361 agcaccttca ggccctccac cacctccacc tccatctatg gcagggtcca cagcaccacc 3421 acctcctcct ccacctccgc ccccaccagg gccaccacct ccccctggcc tgccttctga 3481 tggtgaccat caagttccag ctccttcagg aaacaaagca gctcttttgg atcaaattag 3541 agagggtgct cagctgaaaa aagtggaaca gaatagccgg cccgtgtcct gctcaggaag 3601 ggatgcgctt ttagaccaga tacgacaggg cattcaattg aaatctgtgt ctgatggcca 3661 agagtccaca ccaccaacac ccgcacccac ttcaggaatt gtgggtgcgc tgatggaagt 3721 gatgcagaaa aggagcaaag ccattcattc ctcagatgaa gatgaagacg atgatgatga 3781 agaagatttt gaggatgatg atgagtggga agacgcaaca aacttctctc tgctgaaaca 3841 agccggagat gtcgaagaga atcctggacc ggaattcgcc accatggaag acgccaaaaa 3901 cataaagaaa ggcccggcgc cattctatcc tcttgaggat ggaaccgctg gagagcaact 3961 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca 4021 tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt tggcagaagc 4081 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct 4141 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa 4201 cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc ctaccgtagt 4261 gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat 4321 ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac 4381 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac cagagtcctt 4441 tgatcgtgac aaaacaattg cactgataat gaactcctct ggatctactg ggttacctaa 4501 gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca gagatcctat 4561 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg 4621 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta 4681 tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt 4741 gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca aatacgattt 4801 atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag tcggggaagc 4861 ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca ctgagactac 4921 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt 4981 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca 5041 gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa acaatccgga 5101 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg 5161 ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta aatacaaagg 5221 ataccaggtg gcccccgctg aattggagtc gatattgtta caacacccca acatcttcga 5281 cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt 5341 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt 5401 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct 5461 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg 5521 aaagtccaaa ttgtgaggat ccgcggccgc aaggatctgc gatcgctccg gtgcccgtca 5581 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 5641 aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 5701 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 5761 tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc 5821 tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct 5881 gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct 5941 caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct 6001 ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct 6061 gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga 6121 cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt 6181 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa 6241 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg 6301 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc 6361 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct 6421 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt 6481 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc 6541 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac 6601 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa 6661 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt 6721 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac 6781 ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc 6841 cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 6901 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 6961 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 7021 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 7081 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 7141 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 7201 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 7261 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 7321 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 7381 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 7441 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 7501 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 7561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 7621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 7681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 7741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 7801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 7861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 7921 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 7981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 8041 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc 8101 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8161 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 8221 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 8281 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8341 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8401 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8461 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8581 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 8641 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 8701 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 8761 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 8821 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 8881 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 8941 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 9001 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 9061 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 9121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 9181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 9241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 9421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9601 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 9661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 9781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 9841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 9901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 9961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 10021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 10081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 10141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 10201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 10261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 10321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 10381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 10441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 10501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 10561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 10621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 10681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 10741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 10801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 10861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 10921 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 10981 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 11041 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 11101 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 11161 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 11221 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 11281 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 11341 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 11401 aaaacgacgg ccagtgccaa gctg //