LOCUS V047515 12905 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V047515 VERSION V047515 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..7737 /label="VIRMA(NM_015496)" /note="VIRMA(NM_015496)" /gene="VIRMA" CDS 7744..7770 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 7774..7800 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 7804..7830 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 7875..8086 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 8099..8367 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 8394..8993 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 9000..9588 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 9662..9895 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 9967..10088 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 10107..10242 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(10280..10296) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 10304..10320 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(10328..10358) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 10373..10394 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(10682..11270) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(11441..12301) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(12302..12406) /gene="bla" /label="AmpR promoter" primer_bind 12880..12896 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcggtg gactcggcga tggagctgtt atttttagat 2341 acttttaaac acccgagcgc tgagcaaagt tctcatatag atgtggttcg ttttccatgt 2401 gtggtttata tcaatgaagt ccgagtcata cccccaggag taagagccca tagcagtctg 2461 ccagacaata gagcatatgg agagacatct ccccatacat ttcaattaga cttattcttc 2521 aacaatgtaa gcaaaccaag tgcccctgtt ttcgataggt tgggaagcct ggaatatgat 2581 gagaatactt ccatcatctt tagacctaac tcaaaggtga atactgatgg tctggtgcta 2641 agaggctggt ataactgtct gacactggca atatatggat cagtggatag agtgataagt 2701 catgacagag actctccacc accaccacct ccaccgccac cacctcccca gccacaacca 2761 agtttgaaaa ggaatccaaa acatgctgat ggggagaaag aagatcagtt taatggaagc 2821 cctccaagac cacagccaag gggaccaaga actcctccag gaccccctcc acctgatgat 2881 gatgaagatg atcctgtgcc tctgccagtg tctggtgaca aggaagagga tgctcctcat 2941 agagaagatt actttgagcc catttctcct gatcggaatt ctgttcccca ggaagggcaa 3001 tattctgatg aaggagaagt agaagaggaa caacaagaag aaggagaaga agatgaagat 3061 gatgtggatg tagaggaaga agaggatgag gatgaggatg atcgacgaac agtagacagt 3121 attcctgagg aggaagagga agatgaagag gaagaaggtg aagaggatga agaaggtgaa 3181 ggggatgatg gttatgaaca aatttccagt gatgaagatg gaattgctga cttggaacgt 3241 gaaacattta agtatccaaa ctttgatgtt gaatacactg ctgaagactt agcttcagtt 3301 cctcctatga catatgatcc atatgacagg gagcttgtac cactcttata cttcagttgt 3361 ccatacaaga ctacttttga aattgaaatc agtagaatga aggatcaagg tccagataaa 3421 gaaaattcag gggcaatcga agcctcagtg aagttaacag aactcttaga tttgtataga 3481 gaagatagag gtgcaaaatg ggtaacagct ttagaagaaa ttccaagttt aataataaaa 3541 gggttaagct atttgcaatt gaaaaacaca aaacaagact cccttggcca gttggtagac 3601 tggaccatgc aagctttaaa tttacaagta gcgcttcgcc aacctatcgc cttaaatgtt 3661 cgacagctca aagctgggac caaattagtg tcctcactag cagaatgtgg ggctcaagga 3721 gttacaggac tgctacaagc aggagtgatc agtggattat ttgaacttct gtttgctgat 3781 cacgtatcat cttctcttaa gttaaatgct tttaaagctt tggacagtgt cattagtatg 3841 acagaaggaa tggaagcttt tttaagaggt aggcagaatg aaaaaagtgg ttatcaaaag 3901 cttctggaac tcatactttt agatcagact gtgagggttg ttactgctgg ttcagctatt 3961 ctccaaaaat gccatttcta tgaagtcttg tcagagatta aaagacttgg tgaccattta 4021 gcagagaaga cttcatctct tcctaaccac agtgaacctg atcacgacac agatgctgga 4081 cttgagagaa caaacccaga atatgaaaat gaggtggaag cttctatgga tatggatctt 4141 ttggaatcct caaatataag tgaaggggaa atagaaaggc ttattaacct cctagaagaa 4201 gtttttcatt taatggaaac tgcccctcat acaatgatcc aacaacctgt taagtctttc 4261 ccaacgatgg cacgaattac tggacctcca gagagggatg atccataccc tgttctcttt 4321 agatatcttc acagtcatca cttcttggag ttggttacct tgcttctgtc aattccagta 4381 acaagtgctc accctggtgt gctgcaagcc acaaaagatg ttttgaagtt tcttgcacag 4441 tcacagaagg gtcttctttt ttttatgtcg gaatatgaag caacaaattt attgatccga 4501 gctctgtgtc acttttatga tcaagatgag gaggaaggtc tccaatctga tggtgttatt 4561 gatgatgcat ttgccttgtg gctacaggac tcaacacaga cattgcaatg tattacagaa 4621 ctgttcagcc attttcagcg ttgtacagcc agtgaagaaa cagaccattc agatctcttg 4681 ggaaccctgc acaatcttta tttgattact tttaatcctg tgggaagatc agctgttggc 4741 catgttttta gtctggagaa aaatctccaa agtcttatta ctctaatgga gtactattcc 4801 aaagaagcct tgggtgattc caaatctaag aagtcagtag cttataatta cgcatgcata 4861 cttattttgg tggtggttca gtcttccagt gatgttcaaa tgctagaaca acatgcagca 4921 tctctcttga agctttgtaa agcagatgaa aataatgcta aattgcaaga acttggcaag 4981 tggcttgaac ctctgaaaaa ccttagattt gaaattaact gcatcccaaa cttaattgag 5041 tatgttaagc agaatatcga taacttgatg accccagaag gagttggcct taccactgcc 5101 ttacgtgttc tctgtaatgt tgcatgccca ccacctcctg ttgaaggtca acagaaagat 5161 ctgaaatgga atcttgccgt tattcagctt ttttctgctg aaggaatgga cacgtttatt 5221 cgagttctgc aaaaattgaa cagtattctg actcagcctt ggaggctcca tgtcaacatg 5281 gggactaccc ttcacagagt tactactatt tcaatggctc gctgcacact cactcttctt 5341 aaaactatgt taacggaact cctgagaggt ggatcctttg agtttaagga catgcgtgtt 5401 ccttcagcgc ttgttacttt acatatgctc ctgtgctcta tccccctctc aggtcgtttg 5461 gatagtgatg aacagaaaat tcagaatgat atcattgata ttttactgac ttttacacaa 5521 ggagttaatg aaaaactcac aatctcagaa gagactctgg ccaataatac ttggtcttta 5581 atgttaaaag aagttctttc ttcaatcttg aaggttcctg aaggattttt ttctggactc 5641 atactccttt cagagctgct gcctcttcca ttgcccatgc aaacaactca ggttattgag 5701 ccacatgata tatcagtggc actcaacacc cgaaaattgt ggagcatgca ccttcatgtt 5761 caagcaaagt tgctccaaga aatagttcgc tctttctctg gcacaacctg ccagcccatt 5821 caacatatgt tacggcgtat ttgtgttcaa ttgtgtgacc ttgcctcacc aactgcactt 5881 ctgattatga gaactgtgtt ggatttgatt gtagaagact tgcaaagcac ttcagaagat 5941 aaagaaaaac agtatactag ccaaaccacc aggttgcttg ctcttcttga tgctctggct 6001 tcacacaaag cttgtaaatt agctattttg catctaatta atggaactat taaaggtgat 6061 gaaagatatg cagagatatt ccaggatctt ttagctttgg tgcggtctcc tggagacagt 6121 gttattcgcc aacagtgtgt tgaatatgtc acatccattt tgcagtctct ctgtgatcag 6181 gacattgcac ttatcttacc aagctcttct gaaggttcta tttctgaact ggagcagctc 6241 tccaattctc taccaaataa agaattgatg acctcaatct gtgactgtct gttggctacg 6301 ctagctaact ctgagagcag ttacaactgt ttactgacat gtgtcagaac aatgatgttt 6361 cttgcagagc atgattatgg attatttcat ttaaaaagtt ctttaaggaa aaacagtagt 6421 gctctgcata gtttactgaa acgagtggtc agcacattta gtaaggacac aggagagctt 6481 gcatcttcat ttttagaatt tatgagacaa attcttaact ctgacacaat tggatgctgt 6541 ggagatgata atggtctcat ggaagtagag ggagctcata catcacggac gatgagtatt 6601 aatgctgcag agttaaaaca gcttctacaa agcaaagaag aaagtccaga aaatttgttc 6661 cttgaactag agaagcttgt tttggaacat tcaaaagatg atgacaatct ggattctttg 6721 ttggacagtg tagttggact taagcagatg ctggagtcat caggtgaccc tttacctctc 6781 agtgaccagg atgtagaacc agtactttca gctccagaat ctcttcagaa tctgtttaac 6841 aataggactg cctatgtgct tgctgatgtc atggatgatc agttgaaatc tatgtggttc 6901 actccatttc aggctgaaga gatagataca gatctggatt tggtaaaggt tgacttaatt 6961 gaactctctg aaaaatgctg tagtgacttt gatttgcact cagaattaga gcgctcattt 7021 ttgtcagaac catcatctcc aggaagaacc aagactacta aaggattcaa acttgggaag 7081 cacaagcatg agacctttat aacgtcaagt ggaaaatctg aatacattga acctgccaaa 7141 agagctcatg ttgtgccacc accaagagga aggggcaggg gaggatttgg acagggtata 7201 cgacctcatg atatttttcg tcagagaaaa cagaacacaa gtagaccacc atctatgcat 7261 gtggatgact ttgttgctgc tgaaagtaaa gaagtggttc ctcaagatgg aatacctcca 7321 ccaaaacggc cactcaaagt atcacagaag atttcttccc gtggtgggtt ttcaggcaat 7381 agaggaggac ggggtgcttt ccacagtcag aataggtttt tcacaccacc tgcttcaaaa 7441 ggaaactaca gtcgtcggga aggaacaaga ggctccagtt ggagtgctca gaatactcct 7501 cgaggaaatt acaatgaaag tcgtggaggc cagagcaatt ttaacagagg ccctcttcca 7561 ccattacgac cccttagttc tacaggttac cgcccaagtc ctcgggaccg tgcttctaga 7621 ggtcgtgggg gacttggacc ttcctgggct agtgcaaata gcggcagtgg aggctcaaga 7681 ggaaagtttg ttagtggagg cagtggtaga ggtcgtcatg tacgctcctt tacacgagga 7741 tcctacccat acgacgtacc tgattatgca acttatcctt atgatgtacc tgattatgct 7801 agctatcctt atgatgtccc agactacgca taggcggccg cgaaggatct gcgatcgctc 7861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 7921 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 7981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 8041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 8101 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 8161 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 8221 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 8281 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 8341 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg 8401 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 8461 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 8521 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 8581 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 8641 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 8701 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 8761 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 8821 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 8881 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 8941 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 9001 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 9061 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 9121 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 9181 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 9241 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 9301 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 9361 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 9421 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 9481 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 9541 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 9601 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 9661 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 9721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 9781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 9841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 9901 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 9961 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 10021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 10081 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 10141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 10261 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 10321 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 10381 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 10441 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 10501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 10561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 10621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 10681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 10741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 10801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 10861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 10921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 10981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 11041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 11101 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 11161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 11221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 11281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 11341 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 11401 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 11461 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 11521 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 11581 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 11641 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 11701 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 11761 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 11821 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 11881 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 11941 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 12001 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 12061 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 12121 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 12181 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 12241 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 12301 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 12361 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 12421 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 12481 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 12541 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 12601 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 12661 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 12721 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 12781 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 12841 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 12901 agctg //