LOCUS       V029204                11055 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029204
VERSION     V029204
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5137
                     /label="Unc5b(NM_029770)"
                     /note="Unc5b(NM_029770)"
                     /gene="Unc5b"
     CDS             5144..5209
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5253..5464
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5477..5745
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5780..6487
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             6497..6550
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6551..7150
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7151..7738
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7812..8045
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8117..8238
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8257..8392
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8430..8446)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8454..8470
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8478..8508)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8523..8544
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8832..9420)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9591..10451)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10452..10556)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11030..11046
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgagggc ccggagcggg gtgcggagcg cgctgctgct
     2341 ggcgctgctg ctttgctggg atccgacacc gagcctagca ggcgttgact ctgctggcca
     2401 ggtgctccca gactcctacc catcagcccc tgcggagcag ctgccgtact tcctattgga
     2461 gccacaggac gcctacatcg taaagaacaa gccagtggaa ctgcactgca gagccttccc
     2521 tgccacgcag atctacttca agtgtaatgg cgagtgggtc agccagaatg accacgtcac
     2581 acaggagagc ctggatgagg ccacaggctt gcgggtgcga gaggtgcaga tcgaggtgtc
     2641 acggcagcaa gtggaggaac tcttcgggct cgaggactac tggtgccagt gcgtggcctg
     2701 gagctcttcg ggaactacca agagtcgccg agcctacatc cgcattgcct acttgcgcaa
     2761 gaactttgac caggagcctc tggccaagga ggtacccttg gatcatgagg tccttctgca
     2821 gtgccgccca ccggagggag tgcctgtggc tgaggtggaa tggctcaaga atgaagatgt
     2881 cattgacccc gctcaggaca ctaacttcct gctcaccatt gaccacaacc tcatcatccg
     2941 ccaggcgcgc ctctcagaca cggccaacta cacctgtgtg gccaagaata tcgtggccaa
     3001 gcgccggagc accacggcca cagtcatcgt ctatgtgaat ggaggctggt ccagctgggc
     3061 agagtggtca ccctgttcca atcgctgtgg ccgaggctgg cagaagcgta ctcggacctg
     3121 caccaatcca gccccactca atggaggcgc cttctgtgag ggacaggcct tccagaagac
     3181 agcttgcacc accgtgtgcc cagtggatgg agcgtggacc gagtggagca agtggtctgc
     3241 ctgcagcaca gagtgtgcgc actggcgcag ccgcgagtgc atggcaccgc caccccagaa
     3301 cggaggccgt gactgcagcg ggacgctact tgactccaag aactgcactg atgggctgtg
     3361 cgtgctgaat cagagaactc taaacgaccc taaaagccac cccctggaga catcgggaga
     3421 tgtggcactg tacgcaggcc ttgtggtggc cgtctttgtg gtggtagcgg ttctcatggc
     3481 cgtgggagtg atcgtatacc ggagaaactg ccgggacttc gacacggaca tcaccgactc
     3541 ctctgcggcc ctcactggtg gcttccaccc tgtcaacttc aagactgcaa ggcccaacaa
     3601 cccgcagctc ctgcacccgt ccgcccctcc agacctaacg gccagtgctg gcatctaccg
     3661 cgggcctgtg tatgccctgc aggactccgc cgacaagatc cccatgacta attcgcccct
     3721 gctggatccc ctgcccagcc tcaagatcaa ggtctataac tccagcacca tcggttctgg
     3781 gtctggcctg gctgatggag ccgacctgct gggtgtcctc ccgccgggca cgtacccagg
     3841 cgatttctcc cgggacaccc atttcctgca cctgcgcagt gccagccttg gttcccagca
     3901 cctcctgggc ctacctcggg accccagcag cagtgtcagc ggcacctttg gttgcctggg
     3961 aggaaggctg agcctccccg gcacaggggt cagcctgttg gtaccaaatg gagccattcc
     4021 ccagggcaag ttctatgacc tgtatctaca tatcaacaag gccgaaagca ccctcccact
     4081 ttcagaaggt tcccagacag tattgagccc ctcggtgacc tgtgggccca caggcctact
     4141 cctgtgccgc cctgtcgtcc tcaccgtgcc ccactgtgct gaagtcatcg ctggagactg
     4201 gatctttcag ctcaagaccc aggcccatca gggccactgg gaggaggtgg tgaccttgga
     4261 tgaggagacc ctcaacacac cctgctactg ccagctggag gctaagtcct gccacatcct
     4321 gctggaccag ctgggtacct acgtattcat gggcgagtcc tactctcgct ctgcagtcaa
     4381 gcggctccag ctggccatct tcgccccagc cctctgcacc tccctggagt atagcctcag
     4441 ggtctactgt ctggaggaca cacctgtagc actgaaggag gtcctggagc tggagaggac
     4501 tctgggtggc tacttggtgg aggagcccaa gcctttgctc tttaaggaca gttaccacaa
     4561 cctacgcctc tccctccatg acatccccca tgcccactgg aggagcaaac tactggccaa
     4621 gtaccaggag attcccttct accacgtctg gaatggcagc cagagagccc tgcactgcac
     4681 tttcaccctg gagaggcata gcctggcctc cacggagttc acctgtaagg tctgcgtgcg
     4741 gcaggtcgaa ggggaaggcc agattttcca gctgcacaca acgttggccg agacgcctgc
     4801 tggctccctg gatgctctct gctctgcccc gggcaatgcc atcaccaccc agctgggacc
     4861 ctatgccttc aagatacccc tgtccatccg ccaaaagatc tgcagcagcc tggacgcccc
     4921 caactcccgg ggcaacgact ggaggctgtt ggcgcagaag ctgtccatgg accggtacct
     4981 aaactacttc gccaccaaag ctagtcccac aggtgtcatc ttagacctct gggaagctcg
     5041 gcaacaggat gacggggacc tcaacagcct ggccagtgcc ttggaggaga tgggcaagag
     5101 tgagatgctg gtagccatgg ccacagatgg cgattgcgga tccgactaca aagaccatga
     5161 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg
     5221 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5281 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     5341 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5401 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5461 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     5521 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     5581 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     5641 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     5701 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     5761 gcctacgcta gacgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     5821 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     5881 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     5941 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6001 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6061 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6121 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6181 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6241 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     6301 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     6361 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     6421 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     6481 gtacaaggga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     6541 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6601 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6661 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6721 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6781 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6841 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6901 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6961 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7021 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7081 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7141 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7201 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7261 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7321 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7381 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7441 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7501 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7561 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7621 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7681 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct
     7741 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7801 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg
     7861 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8041 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8101 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
     8281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8341 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     8401 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8461 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8521 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8581 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8641 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8701 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8761 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8821 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8881 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8941 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9001 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9061 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9121 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9181 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9241 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9301 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9361 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9421 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9481 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9541 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9601 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9661 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9721 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9781 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9841 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9901 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9961 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10021 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10081 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10141 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10201 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10261 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10321 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10381 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10441 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10501 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10561 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10621 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10681 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10741 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    10801 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    10861 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    10921 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10981 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    11041 gccagtgcca agctg
//
