LOCUS       V036570                11500 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036570
VERSION     V036570
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5526
                     /label="Ube3b(NM_054093)"
                     /note="Ube3b(NM_054093)"
                     /gene="Ube3b"
     CDS             5533..5598
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5642..5853
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5866..6134
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6163..6172
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6193..6855
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6925..6978
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6979..7578
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7579..8167
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8241..8474
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8546..8667
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8707..8842
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8875..8891)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8899..8915
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8923..8953)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8968..8989
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9277..9865)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10036..10896)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10897..11001)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11475..11491
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tcactgtatc tcagacctcc
     2341 agagcatggt tcatcgacag agcccggcag gcacgagaag agaggctggt gcagaaggag
     2401 cgggagcgct cagcggtgac gatccaggcc ctagtccgca gtttcctctg ccggaggcga
     2461 ctgcacagag acatcaggaa agagattgat gagtttttta gtgctgatga gtctgggtcc
     2521 agtaaaagaa gtgcactttg tatttttaag attgccagga gactgctgtt tatatgcaaa
     2581 actaccgagg acagcgagag actggagaag ttgtgtcgaa gcatcctgaa cagcatggat
     2641 gccgagaatg agccaaaggt gtggtacgtg tccctggctc tctccaagga cctcacgctg
     2701 ctgtggatta agcagatcaa gagcatcctg tggcactgct gtgagcttct ggggcagctc
     2761 aagccagaga tcttacagga ctccaggctc atcacgctgt acctcaccat gcttgtgacc
     2821 ttcacagaca cttcaacatg gaagattctc cggggaaaag gtgagagcct tcggccagcc
     2881 ctgaaccaca tttgcgcaaa tataatgggc catctcaacc agcgtggact ttactctgta
     2941 ttgcaggtac tgctgacccg aggcctggcc aggccccggc cctgtctgtc caaaggcatg
     3001 ttaacagcag ccttctctct ggccctgcgc cctgtggttg ctgcgcagtt ctcagacaat
     3061 cttatgcgac cattcatcat ccacgtcatg tcggtacctg ctctcgtggc tcatctcagc
     3121 accgtggccc ctgagcgcct cggcgtttta gagtcccatg acatgcttcg taaattcatc
     3181 gtcttcctga gagatagaga ccgatgccgc gatgcttgtg agagtttaga aggctgccat
     3241 accctgtgtc tcatgggcaa tctcctgcac ttgggctccc tcagcctcag gctgttagag
     3301 gaagagatgg atgggtttgt gagcgcgctc acgcagatgc tgtgctactg tcagaagtac
     3361 gtggctcaga agaagtccaa cctgacccac tggcaccctg tgctcggctg gttttcccaa
     3421 cctgtggatt acggcctcaa tgattccatg tacctgatca ccaagcagct gcagttcctg
     3481 tgggcggtgc ctctcatccg cattctcttc agtgacatcc tgagcaggaa gctgctggag
     3541 catgcagagc cggccccggt gcaaccgcag ccgtcctcac cgcagacggt gctccccgtg
     3601 aagagcctcc tcaagcgtgc gtttcagaag tcggcttcgg ttcggaacat cctcaggcct
     3661 gttgggggcc gccgtgtcga ctccgctgag gtgcggaagg tctgcaacat ttgcgtgctc
     3721 taccagacct cgctgacgac cctcacccag atccgcctgc agatcctcac aggtcttacg
     3781 tacctggatg acctgctgcc caagctctgg gcgtttatct gtgagctggg gccccacggc
     3841 gggctcaagc tcttcctgga gtgcctgaac aatgacactg gagagtccaa gcagctcctg
     3901 gccatgctga tgctcttctg tgactgttcc cggcacctca tcaccatcct cgatgacatt
     3961 gaagtttatg aagaacagat ctccttcaag ctggaagagc tggtcaccat ctcctcgttc
     4021 ctcaattcct tcgtgtttaa gatgatctgg gacggaattg tagagaatgc caagggtgag
     4081 accttggagc tgttccagtc cgtccatggc tggctgatgg tgctgtatga gcgagactgc
     4141 cgccggcgct ttgctccgga ggaccactgg ctacggaggg acctcaagcc tggtgtgctc
     4201 ttccaggagc tcgacaagga ccgaaggagg gctcagctgg tcctgcagca catcccccac
     4261 gtcgtgcctc acaagaacag agttcttctg ttccggaaca tggttatcaa ggagaaagag
     4321 aagctggggc ttgtggagac cagctctgcc tctcctcacg tcacccacat caccatccga
     4381 aggtcccgga tgttggagga cggctacgaa cagctccggc agctttccca gcatgccatg
     4441 aagggtgtga tccgagtgaa gtttgtcaac gacctcgggg tggacgaggc tgggattgac
     4501 caggatggtg tcttcaagga gtttctggaa gagatcatca agagggtgtt tgaccccgca
     4561 ctcaacctgt tcaagacaac cagcggggat gagcggctgt acccatcacc cacgtcctac
     4621 attcacgaga attacctgca gctcttcgag tttgtgggga agatgctggg aaaggcagtg
     4681 tatgagggga tcgtggtaga tgtgcccttt gcctccttct tcctgagcca gatgctcggg
     4741 caccaccaca gcgtcttcta cagctccgtg gatgagcttc cctccctgga ctcggagttt
     4801 tataaaaacc tcacctccat caagcgctac gatggagaca tcgcagacct cgggctgacg
     4861 ctgtcttacg atgaagacgt catgggtcag ctcgtctgcc acgaactggt tcccggaggg
     4921 aagaccatcc cggttacaga tgagaacaaa attagctaca tccatctgat ggcgcatttc
     4981 cgaatgcaca ctcaaattaa aaaccagaca gctgctctta tcagtggctt ccgttccatc
     5041 atcaagcccg agtggatccg aatgttctct acccctgagc ttcagcgcct catctccggg
     5101 gacaacgctg agatagatct ggaagactta aagaagcaca cagtgtacta cggtggcttt
     5161 catgggagcc acagggtcat catctggctc tgggatatcc tggcttccga cttcacgcct
     5221 gaagaaagag ccatgttcct gaagtttgtg accagctgct cccggccccc gctcctagga
     5281 ttcgcctacc tcaagcctcc gttctccatc cgctgtgtgg aagtatcaga tgaccaggac
     5341 actggggata ccctgggcag cgtcctcagg ggcttcttca ccatccgcaa gagggagcca
     5401 ggggggcgtc tgcccacatc ttccacttgc ttcaacctgc ttaagctgcc caactacagc
     5461 aagaagagcg tactgcgaga gaaactacgc tatgccatca gcatgaacac gggctttgag
     5521 ctctccggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5581 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5641 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5701 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5761 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5821 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5881 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5941 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6001 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6061 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6121 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6181 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6241 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6301 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6361 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6421 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6481 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6541 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6601 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6661 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6721 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6781 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6841 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6901 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6961 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7021 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7081 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7141 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7201 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7261 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7321 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7381 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7441 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7501 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7561 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7621 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7681 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7741 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7801 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7861 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7921 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7981 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8041 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8101 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8161 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8221 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8281 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8341 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8401 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8461 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8521 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8581 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8641 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8701 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8761 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8821 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8881 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8941 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9001 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9061 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10021 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10081 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10141 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10201 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10261 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10321 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10381 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10441 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10501 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10561 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10621 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    11041 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    11101 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11161 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11221 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11281 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11461 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
