LOCUS       V028854                10753 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028854
VERSION     V028854
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5607
                     /label="USP7(NM_003470)"
                     /note="USP7(NM_003470)"
                     /gene="USP7"
     CDS             5614..5679
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5723..5934
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5947..6215
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6242..6841
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6848..7436
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7510..7743
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7815..7936
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7955..8090
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8128..8144)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8152..8168
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8176..8206)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8221..8242
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8530..9118)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9289..10149)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10150..10254)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10728..10744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgaaccac cagcagcagc agcagcagca gaaagcgggc
     2341 gagcagcagt tgagcgagcc cgaggacatg gagatggaag cgggagatac agatgaccca
     2401 ccaagaatta ctcagaaccc tgtgatcaat gggaatgtgg ccctgagtga tggacacaac
     2461 accgcggagg aggacatgga ggatgacacc agttggcgct ccgaggcaac ctttcagttc
     2521 actgtggagc gcttcagcag actgagtgag tcggtcctta gccctccgtg ttttgtgcga
     2581 aatctgccat ggaagattat ggtgatgcca cgcttttatc cagacagacc acaccaaaaa
     2641 agcgtaggat tctttctcca gtgcaatgct gaatctgatt ccacgtcatg gtcttgccat
     2701 gcacaagcag tgctgaagat aataaattac agagatgatg aaaagtcgtt cagtcgtcgt
     2761 attagtcatt tgttcttcca taaagaaaat gattggggat tttccaattt tatggcctgg
     2821 agtgaagtga ccgatcctga gaaaggattt atagatgatg acaaagttac ctttgaagtc
     2881 tttgtacagg cggatgctcc ccatggagtt gcgtgggatt caaagaagca cacaggctac
     2941 gtcggcttaa agaatcaggg agcgacttgt tacatgaaca gcctgctaca gacgttattt
     3001 ttcacgaatc agctacgaaa ggctgtgtac atgatgccaa ccgaggggga tgattcgtct
     3061 aaaagcgtcc ctttagcatt acaaagagtg ttctatgaat tacagcatag tgataaacct
     3121 gtaggaacaa aaaagttaac aaagtcattt gggtgggaaa ctttagatag cttcatgcaa
     3181 catgatgttc aggagctttg tcgagtgttg ctcgataatg tggaaaataa gatgaaaggc
     3241 acctgtgtag agggcaccat acccaaatta ttccgcggca aaatggtgtc ctatatccag
     3301 tgtaaagaag tagactatcg gtctgataga agagaagatt attatgatat ccagctaagt
     3361 atcaaaggaa agaaaaatat atttgaatca tttgtggatt atgtggcagt agaacagctc
     3421 gatggggaca ataaatacga cgctggggaa catggcttac aggaagcaga gaaaggtgtg
     3481 aaattcctaa cattgccacc agtgttacat ctacaactga tgagatttat gtatgaccct
     3541 cagacggacc aaaatatcaa gatcaatgat aggtttgaat tcccagagca gttaccactt
     3601 gatgaatttt tgcaaaaaac agatcctaag gaccctgcaa attatattct tcatgcagtc
     3661 ctggttcata gtggagataa tcatggtgga cattatgtgg tttatctaaa ccccaaaggg
     3721 gatggcaaat ggtgtaaatt tgatgacgac gtggtgtcaa ggtgtactaa agaggaagca
     3781 attgagcaca attatggggg tcacgatgac gacctgtctg ttcgacactg cactaatgct
     3841 tacatgttag tctacatcag ggaatcaaaa ctgagtgaag ttttacaggc ggtcaccgac
     3901 catgatattc ctcagcagtt ggtggagcga ttacaagaag agaaaaggat cgaggctcag
     3961 aagcggaagg agcggcagga agcccatctc tatatgcaag tgcagatagt cgcagaggac
     4021 cagttttgtg gccaccaagg gaatgacatg tacgatgaag aaaaagtgaa atacactgtg
     4081 ttcaaagtat tgaagaactc ctcgcttgct gagtttgttc agagcctctc tcagaccatg
     4141 ggatttccac aagatcaaat tcgattgtgg cccatgcaag caaggagtaa tggaacaaaa
     4201 cgaccagcaa tgttagataa tgaagccgac ggcaataaaa caatgattga gctcagtgat
     4261 aatgaaaacc cttggacaat attcctggaa acagttgatc ccgagctggc tgctagtgga
     4321 gcgaccttac ccaagtttga taaagatcat gatgtaatgt tatttttgaa gatgtatgat
     4381 cccaaaacgc ggagcttgaa ttactgtggg catatctaca caccaatatc ctgtaaaata
     4441 cgtgacttgc tcccagttat gtgtgacaga gcaggattta ttcaagatac tagccttatc
     4501 ctctatgagg aagttaaacc gaatttaaca gagagaattc aggactatga cgtgtctctt
     4561 gataaagccc ttgatgaact aatggatggt gacatcatag tatttcagaa ggatgaccct
     4621 gaaaatgata acagtgaatt acccaccgca aaggagtatt tccgagatct ctaccaccgc
     4681 gttgatgtca ttttctgtga taaaacaatc cctaatgatc ctggatttgt ggttacgtta
     4741 tcaaatagaa tgaattattt tcaggttgca aagacagttg cacagaggct caacacagat
     4801 ccaatgttgc tgcagttttt caagtctcaa ggttataggg atggcccagg taatcctctt
     4861 agacataatt atgaaggtac tttaagagat cttctacagt tcttcaagcc tagacaacct
     4921 aagaaacttt actatcagca gcttaagatg aaaatcacag actttgagaa caggcgaagt
     4981 tttaaatgta tatggttaaa cagccaattt agggaagagg aaataacact atatccagac
     5041 aagcatgggt gtgtccggga cctgttagaa gaatgtaaaa aggccgtgga gcttggggag
     5101 aaagcatcag ggaaacttag gctgctagaa attgtaagct acaaaatcat tggtgttcat
     5161 caagaagatg aactattaga atgtttatct cctgcaacga gccggacgtt tcgaatagag
     5221 gaaatccctt tggaccaggt ggacatagac aaagagaatg agatgcttgt cacagtggcg
     5281 catttccaca aagaggtctt cggaacgttc ggaatcccgt ttttgctgag gatacaccag
     5341 ggcgagcatt ttcgagaagt gatgaagcga atccagagcc tgctggacat ccaggagaag
     5401 gagtttgaga agtttaaatt tgcaattgta atgatgggcc gacaccagta cataaatgaa
     5461 gacgagtatg aagtaaattt gaaagacttt gagccacagc ccggtaatat gtctcatcct
     5521 cggccttggc tagggctcga ccacttcaac aaagccccaa agaggagtcg ctacacttac
     5581 cttgaaaagg ccattaaaat ccataacgga tccgactaca aagaccatga cggtgattat
     5641 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     5701 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5761 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     5821 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5881 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5941 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     6001 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     6061 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     6121 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     6181 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     6241 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt
     6301 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga
     6361 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6421 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6481 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6541 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6601 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6661 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6721 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6781 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6841 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6901 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6961 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7021 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7081 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7141 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7201 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7261 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7321 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7381 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7441 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7501 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     7561 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7741 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7801 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7861 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7921 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     7981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8041 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     8101 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10261 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    10321 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    10381 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    10441 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    10501 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10561 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    10621 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    10681 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    10741 cagtgccaag ctg
//
