LOCUS       V028541                10816 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028541
VERSION     V028541
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5670
                     /label="USP43(NM_153210)"
                     /note="USP43(NM_153210)"
                     /gene="USP43"
     CDS             5677..5742
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5786..5997
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6010..6278
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6305..6904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6911..7499
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7573..7806
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7878..7999
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8018..8153
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8191..8207)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8215..8231
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8239..8269)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8284..8305
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8593..9181)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9352..10212)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10213..10317)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10791..10807
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggacctg ggccccgggg acgcggcagg agggggaccg
     2341 ctcgcgcccc ggccccgccg ccgccgctcc ctgcgccgcc tgttcagccg cttcctgctg
     2401 gcgctgggca gccgctcacg ccccggggac tcaccgcccc ggccccagcc gggacactgt
     2461 gatggcgacg gtgagggggg cttcgcctgc gccccgggcc cagttccagc ggcccccggg
     2521 agccccgggg aggaacgccc gcccggaccc cagccccagc tccagctccc cgccggcgat
     2581 ggggcgcggc cgccgggcgc tcagggcttg aagaaccacg gcaacacctg tttcatgaac
     2641 gcggtggtgc agtgtctcag caacaccgac ctgctggccg agttcctggc gctggggcgc
     2701 taccgggcgg ctccgggccg cgccgaggtc accgagcagc tggcggcgct ggtgcgcgcg
     2761 ctctggactc gcgaatacac gccccaactt tccgcggagt tcaagaatgc agtttccaag
     2821 tacggctctc agttccaagg caattcccag cacgacgccc tggaattcct gctctggttg
     2881 ctggatcgtg tacatgagga cctggagggt tcatcccgag ggccggtgtc ggagaagctt
     2941 ccgcctgaag ccactaaaac ctctgagaac tgcctgtcac catcagctca gcttcctcta
     3001 ggtcaaagct ttgtgcaaag ccactttcaa gcacaatata gatcttcctt gacttgtccc
     3061 cactgcctga aacagagcaa cacctttgat cctttcctgt gtgtgtccct acctatcccc
     3121 ttgcgccaga cgaggttctt gagtgtcacc ttggtcttcc cctctaagag ccagcggttc
     3181 ctgcgggttg gcctggccgt gccgatcctc agcacagtgg cagccctgag gaagatggtt
     3241 gcagaggaag gaggcgtccc tgcagatgag gtgatcttgg ttgaactgta tcccagtgga
     3301 ttccagcggt ctttctttga tgaagaggac ctgaatacca tcgcagaggg agataatgtg
     3361 tatgcctttc aagttcctcc ctcacccagc caggggactc tctcagctca tccactgggt
     3421 ctgtcggcct ccccacgcct ggcagcccgt gagggccagc gattctccct ctctctccac
     3481 agtgagagca aggtgctaat cctcttctgt aacttggtgg ggtcagggca gcaggctagc
     3541 aggtttgggc cacccttcct gataagggaa gacagagctg tttcctgggc ccagctccag
     3601 cagtctatcc tcagcaaggt ccgccatctt atgaagagtg aggcccctgt acagaacctg
     3661 gggtctctgt tctccatccg tgttgtggga ctctctgtgg cctgcagcta tttgtctccg
     3721 aaggacagtc ggcccctctg tcactgggca gttgacaggg ttttgcatct caggaggcca
     3781 ggaggccctc cacatgtcaa gctggcggtg gagtgggata gctctgtcaa ggagcgcctg
     3841 ttcgggagcc tccaggagga gcgagcgcag gatgccgaca gtgtgtggca gcagcagcag
     3901 gcgcatcagc agcacagctg taccttggat gaatgttttc agttctacac caaggaggag
     3961 cagctggccc aggatgacgc ctggaagtgt cctcactgcc aagtcctgca gcaggggatg
     4021 gtgaagctga gtttgtggac gctgcctgac atcctcatca tccacctcaa aaggttctgc
     4081 caggtgggcg agagaagaaa caagctctcc acgctggtga agtttccgct ctctggactc
     4141 aacatggctc cccatgtggc ccagagaagc accagccctg aggcaggact gggcccctgg
     4201 ccttcctgga agcagccgga ctgcctgccc accagttacc cgctggactt cctgtacgac
     4261 ctgtatgccg tctgcaacca ccatggcaac ctgcaaggtg ggcattacac agcctactgc
     4321 cggaactctc tggatggcca gtggtacagt tatgatgaca gcacggtgga accgcttcga
     4381 gaagatgagg tcaacaccag aggggcttat atcctgttct atcagaagcg gaacagcatc
     4441 cctccctggt cagccagcag ctccatgaga ggctctacca gctcctccct gtctgatcac
     4501 tggctcttac ggctcgggag ccacgctggc agcacaaggg gaagcctgct gtcctggagc
     4561 tctgccccct gcccctccct gccccaggtt cctgactctc ccatcttcac caacagcctc
     4621 tgcaatcagg aaaagggagg gttggagccc aggcgtttgg tacggggcgt gaaaggcaga
     4681 agcattagca tgaaggcacc caccacttcc cgagccaagc agggaccatt caagaccatg
     4741 cctctgcggt ggtcctttgg atccaaggag aaaccaccag gtgcctccgt cgagttggtg
     4801 gagtacttgg aatccagacg aagacctcgg tccacgagcc agtccattgt gtcgctgttg
     4861 acgggcactg cgggtgagga tgagaagtca gcatcgccga ggtccaacgt cgcccttcct
     4921 gctaacagcg aagatggtgg gcgggccatt gaaagaggtc cagccggggt gccctgtccc
     4981 tcggctcaac ccaaccactg tctggcccct ggaaactcag atggtccaaa cacagcaagg
     5041 aaactcaagg aaaatgcagg gcaggacatc aagcttccca gaaagtttga cctgcctctc
     5101 actgtgatgc cttcagtgga gcatgagaaa ccagctcgac cggagggcca gaaggccatg
     5161 aactggaagg agagcttcca gatgggaagc aaaagcagcc caccctcccc ctatatggga
     5221 ttctctggaa acagcaaaga cagtcgccga ggcacctctg agctagacag acccctgcag
     5281 gggacactca cccttctgag gtccgtgttt cggaagaagg agaacaggag gaatgagagg
     5341 gcagaggtct ctccacaggt gccccccgtc tccctggtga gtggcgggct gagccctgcc
     5401 atggacgggc aggctccagg ctcacctcct gccctcagga tcccagaggg cctggccagg
     5461 ggcctgggca gccggctcga gagggatgtc tggtcagccc ccagctctct ccgcctccct
     5521 cgtaaagcca gcagggcccc gagaggcagt gcactgggca tgtcacaaag gactgttcca
     5581 ggggagcagg cttcttatgg cacctttcag agagtcaaat atcacactct ttctttaggt
     5641 cgaaagaaaa ccttaccgga gtccagcttt ggatccgact acaaagacca tgacggtgat
     5701 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagcggcc gcgaaggatc
     5761 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5821 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5881 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5941 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     6001 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     6061 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     6121 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     6181 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     6241 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     6301 ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     6361 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     6421 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6481 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6541 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6601 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6661 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6721 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6781 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6841 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6901 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6961 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7021 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7081 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7141 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7201 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7261 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7321 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7381 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7441 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7501 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7561 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     7621 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7681 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7741 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7801 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7861 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7921 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7981 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     8041 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8101 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     8161 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8761 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9001 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10321 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10561 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10621 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10681 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10741 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10801 ggccagtgcc aagctg
//
