LOCUS       V034446                11056 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034446
VERSION     V034446
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5082
                     /label="USP29(NM_020903)"
                     /note="USP29(NM_020903)"
                     /gene="USP29"
     CDS             5089..5154
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5198..5409
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5422..5690
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5719..5728
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5749..6411
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6481..6534
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6535..7134
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7135..7723
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7797..8030
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8102..8223
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8263..8398
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8431..8447)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8455..8471
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8479..8509)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8524..8545
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8833..9421)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9592..10452)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10453..10557)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11031..11047
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tatctctaaa ggtatgtgga
     2341 ttcatccaaa tttggagcca gaagactggg atgactaagc tgaaagaagc tctcattgaa
     2401 acagtgcaaa gacaaaagga aattaaactg gtggtcactt tcaaatctgg aaaatttata
     2461 agaatttttc agctgagcaa caacattaga agtgtggtcc ttagacattg taaaaaaaga
     2521 caaagtcacc tgcgtttaac tttgaaaaac aacgtgttct tgtttattga caaattatcc
     2581 tacagagatg ctaaacagtt gaatatgttc ctggacataa tccaccaaaa caaatctcag
     2641 caacccatga aatctgatga tgattggagt gtgtttgaaa gcaggaatat gctgaaggaa
     2701 attgacaaaa cttcatttta cagcatttgt aacaagccaa gttatcagaa gatgcctttg
     2761 tttatgtcaa aatcaccaac acatgtgaaa aaggggatat tagaaaatca aggtgggaag
     2821 gggcaaaaca cactatcatc tgatgtacag acaaatgagg acattctgaa ggaagataac
     2881 cctgtaccaa acaagaaata taagacagat tccttgaaat atatacaaag caataggaag
     2941 aacccatcaa gtttagagga tttagaaaaa gatagagatt tgaaactcgg gccttcattc
     3001 aataccaact gtaatggaaa tcctaaccta gatgagactg ttcttgcaac ccagactctc
     3061 aatgccaaaa atggtttgac atctccattg gaaccagagc acagccaggg tgacccaaga
     3121 tgcaacaaag cccaggtgcc tcttgactct cattcacagc aactgcagca ggggttcccc
     3181 aatttgggaa acacctgtta catgaatgca gttttacaat cgctatttgc aattccatct
     3241 tttgctgatg acttactcac tcaaggtgtc ccatgggaat atattccctt tgaggctctt
     3301 attatgacct tgacccagct gcttgctttg aaagatttct gtagtacaaa gatcaagaga
     3361 gaattacttg ggaatgttaa aaaagtcatt tcagcagttg cagaaatatt ttctggcaac
     3421 atgcagaatg atgctcatga gtttttaggt cagtgtttag accagctgaa agaagacatg
     3481 gaaaaattaa atgccacttt gaatactggg aaagaatgtg gggatgaaaa ttcatctcca
     3541 caaatgcatg ttggtagtgc tgccaccaaa gtgtttgttt gccctgttgt tgctaatttt
     3601 gagtttgaat tgcagctctc ccttatttgt aaagcttgtg gtcatgctgt tctcaaggta
     3661 gaacctaata attatctctc catcaacctg caccaagaaa caaaaccact tcctttgtcc
     3721 attcagaatt ctttagatct tttctttaaa gaagaagagc ttgaatataa ctgtcagatg
     3781 tgtaagcaga agagttgtgt tgcaaggcat acatttagta ggctctccag ggtccttatc
     3841 attcatctga aacgctatag cttcaacaat gcttggttgc tggtgaagaa taacgagcaa
     3901 gtttatattc ccaaatcttt aagtttatct tcttattgca atgaaagcac caaaccacct
     3961 cttcccttga gcagtagtgc acctgttggg aaatgtgaag tcctggaagt ctctcaggag
     4021 atgatttctg agatcaacag cccattgaca ccatcaatga agctgacctc agaatccagt
     4081 gattccctgg ttctacccgt tgaaccagac aagaatgccg acctacaaag attccagaga
     4141 gactgtggag atgcaagcca agagcagcat cagagagacc tggaaaatgg ctctgcacta
     4201 gagtcagaat tggtccactt tagagatagg gcaatcggtg aaaaggagct tccagtggct
     4261 gactcactga tggaccaggg agacatttct cttcctgtga tgtatgaaga tggagggaag
     4321 ctgatcagca gcccagacac aaggcttgtc gaggttcatc ttcaagaggt gcctcaacat
     4381 ccagaacttc agaagtatga gaaaaccaat acattcgtag agttcaattt tgacagtgtc
     4441 actgagtcca ccaatggctt ttatgactgt aaagaaaaca ggattccaga aggatctcaa
     4501 ggaatggctg aacagctcca gcagtgtatt gaggagagca tcatagatga atttcttcag
     4561 caggcaccac ctccaggtgt taggaagctg gatgcccagg aacatacaga agagaccctc
     4621 aatcagtcta cagaattaag acttcaaaag gctgacctga atcaccttgg ggcactgggt
     4681 tctgacaacc caggaaacaa aaacatttta gatgcagaga acacaagagg tgaagccaag
     4741 gaactaacaa gaaacgtgaa gatgggggat cctctccagg cctacagact catcagtgtt
     4801 gtcagccata tcgggagctc cccaaattca ggccattaca tcagcgatgt gtatgacttt
     4861 cagaagcagg cctggttcac atacaacgat ctatgtgtat cagaaatctc agagaccaaa
     4921 atgcaggagg cgaggcttca ctctgggtat atcttctttt acatgcacaa tgggattttt
     4981 gaggagctgt taagaaaagc agagaactct cggctaccta gcacacaggc aggggtgatc
     5041 cctcaggggg aatacgaagg tgactctttg tacagacctg ctggatccga ctacaaagac
     5101 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5161 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5221 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5281 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5341 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5401 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5461 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5521 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5581 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5641 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5701 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5761 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5821 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5881 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5941 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6001 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6061 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6121 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6181 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6241 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6301 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6361 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6421 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6481 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6541 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6601 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6661 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6721 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6781 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6841 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6901 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6961 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7021 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7081 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7141 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7201 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7261 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7321 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7381 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7441 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7501 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7561 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7621 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7681 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7741 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7801 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8041 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8101 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8161 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8221 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8341 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8401 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     8461 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8521 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8581 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8641 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8701 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8761 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8821 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8881 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8941 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9001 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9241 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9421 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9481 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9541 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9601 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9661 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9721 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9781 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9841 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9901 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9961 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10021 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10081 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10141 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10201 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10261 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10321 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10381 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10441 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10501 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10561 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10621 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10681 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10741 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10801 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10861 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10921 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10981 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    11041 ggccagtgcc aagctg
//
