LOCUS       V039205                11075 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039205
VERSION     V039205
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5076
                     /label="USP11(NM_001371072)"
                     /note="USP11(NM_001371072)"
                     /gene="USP11"
     CDS             5083..5109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5113..5139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5143..5169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5234..5445
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5458..5726
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5755..5764
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5785..6447
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6517..6570
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6571..7170
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7171..7758
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7832..8065
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8137..8258
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8277..8412
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8450..8466)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8474..8490
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8498..8528)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8543..8564
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8852..9440)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9611..10471)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10472..10576)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11050..11066
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgacggtcgc agcaaatcca
     2341 gctgctgctg cggcggctgt ggcggcggca gcggcggtga ctgaggatag agagccacag
     2401 cacgaggagc tgccaggcct ggacagccag tggcgccaga tagaaaacgg cgagagtggg
     2461 cgagaacgtc cactgcgggc cggcgaaagc tggttccttg tggagaagca ctggtataag
     2521 cagtgggagg catacgtgca gggaggggac caggactcca gcaccttccc tggctgcatc
     2581 aacaatgcca cactctttca agatgagata aactggcgcc tcaaggaggg actggtggaa
     2641 ggcgaggatt atgtgctgct cccagcagct gcttggcatt acctggtcag ctggtatggt
     2701 ctagagcatg gccagccacc cattgaacgc aaggtcatag agctgcccaa catccagaag
     2761 gtcgaagtgt acccagtaga actgctgctt gtccggcaca atgatttggg caaatctcac
     2821 actgttcagt tcagccatac cgattctatt ggcctagtat tgcgcacagc tcgggagcgg
     2881 tttctggtgg agccccagga agacactcgg ctttgggcca agaactcaga aggctctttg
     2941 gataggttgt atgacacaca catcacggtt ctcgatgcgg cccttgagac tgggcagttg
     3001 atcatcatgg agacccgcaa gaaagatggc acttggccca gcgcacagct gcatgtcatg
     3061 aacaacaaca tgtcggaaga ggatgaggac ttcaagggtc agccaggcat ctgtggcctc
     3121 accaatctgg gcaacacgtg cttcatgaac tcggccctgc agtgcctcag caatgtgcca
     3181 cagctcaccg agtacttcct caacaactgc tacctggagg agctcaactt ccgcaaccca
     3241 ctgggcatga agggtgagat cgcagaggcc tatgcagacc tggtgaagca ggcgtggtct
     3301 ggccaccacc gctccattgt gccacatgtg ttcaagaaca aggttggcca ttttgcatcc
     3361 caatttctgg gctaccagca gcatgactct caggagctgc tgtcattcct cctggacggg
     3421 ctgcatgagg accttaatcg ggtgaagaag aaggagtatg tggagctgtg cgatgctgct
     3481 gggcgaccgg atcaggaggt ggcacaggag gcatggcaaa accacaaacg gcggaacgat
     3541 tctgtgatcg tggacacttt ccacggcctc ttcaagtcca cgctggtgtg ccccgattgt
     3601 ggcaatgtat ctgtgacctt cgaccccttc tgctacctca gtgttccact gcctatcagc
     3661 cacaagaggg tcttggaggt cttctttatc cccatggatc cgcgccgcaa gccagagcag
     3721 caccggctcg tggtccccaa gaaaggcaag atctcggatc tatgtgtggc tctgtccaaa
     3781 cacacgggca tctcgccaga gaggatgatg gtggctgatg tcttcagtca ccgcttctat
     3841 aagctctatc agctagagga gcctctgagc agcatcttgg accgtgatga tatcttcgtc
     3901 tatgaggtgt caggtcgcat tgaggccatt gagggctcaa gagaggacat cgtggttcct
     3961 gtctacctgc gggagcgcac ccctgcccgt gactacaaca actcctacta cggcctgatg
     4021 ctttttggac accccctcct ggtatcagtg ccccgggacc gcttcacctg ggagggcctg
     4081 tataacgtcc tgatgtaccg gctctcacgc tacgtgacca aacccaactc agatgatgag
     4141 gacgatgggg atgagaaaga agatgacgag gaggataaag atgacgtccc tgggccctca
     4201 actgggggca gcctccgaga ccctgagcca gagcaggctg ggcccagctc tggagtcacg
     4261 aacaggtgcc cgttcctcct ggacaattgc cttggcacat ctcagtggcc cccaaggcga
     4321 cgacgcaagc agctgttcac cctgcagacg gtgaactcca atgggaccag cgaccgcaca
     4381 acctcccctg aagaagtcca tgcccagccg tacattgcta tcgactggga gccagagatg
     4441 aagaagcgtt actatgacga ggtagaggct gagggctacg tgaagcatga ctgcgtcggg
     4501 tacgtgatga agaaggctcc cgtgcggctg caggagtgca ttgagctctt caccactgtg
     4561 gagaccctgg agaaggaaaa cccctggtac tgcccttcct gcaagcagca ccagctggca
     4621 accaagaagc tggacctgtg gatgctgccg gagattctca tcatccacct gaaacgcttt
     4681 tcctacacca agttctcccg agagaagctg gacaccctcg tggagtttcc tatccgggac
     4741 ctggacttct ctgagtttgt catccagcca cagaatgagt cgaatccgga gctgtacaaa
     4801 tatgacctca tcgcggtttc caaccattat gggggcatgc gtgatggaca ctacacaaca
     4861 tttgcctgca acaaggacag cggccagtgg cactactttg atgacaacag cgtctcccct
     4921 gtcaatgaga atcagatcga gtccaaggca gcctatgtcc tcttctacca acgccaggac
     4981 gtggcgcgac gcctgctgtc cccggccggc tcatctggcg ccccagcctc ccctgcctgc
     5041 agctccccac ccagctctga gttcatggat gttaatctcg agtacccata cgacgtacct
     5101 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca
     5161 gactacgcat agggatccct gcgatatcca gcgcggccgc gaaggatctg cgatcgctcc
     5221 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5281 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5341 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5401 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5461 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5521 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5581 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5641 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5701 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5761 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5821 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5881 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5941 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     6001 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     6061 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     6121 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     6181 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     6241 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6301 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6361 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6421 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6481 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6541 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6601 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6661 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6721 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6781 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6841 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6901 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6961 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7021 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7081 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7141 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     7201 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7261 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7321 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7381 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7441 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7501 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7561 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7621 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7681 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc
     7741 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7801 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7861 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7921 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7981 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8041 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8101 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8281 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8341 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8401 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     8461 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8521 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8581 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8641 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8701 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8761 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8821 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8881 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8941 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9001 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9061 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9121 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9181 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9241 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9301 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9361 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9481 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9541 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9601 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9661 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9721 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9781 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9841 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9901 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9961 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10021 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10081 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10141 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10201 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10261 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10321 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10381 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10441 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10501 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10561 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10621 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10681 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10741 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10801 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10861 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10921 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10981 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    11041 cacgacgttg taaaacgacg gccagtgcca agctg
//
