LOCUS       V021206                10632 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021206
VERSION     V021206
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2279..2288
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2288..2359
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             2366..4747
                     /label="UFL1(NM_015323)"
                     /note="UFL1(NM_015323)"
                     /gene="UFL1"
     promoter        4791..5002
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5015..5283
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5312..5321
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5342..6004
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6074..6127
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6128..6727
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6728..7315
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7389..7622
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7694..7815
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7834..7969
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8007..8023)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8031..8047
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8055..8085)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8100..8121
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8409..8997)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9168..10028)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10029..10133)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10607..10623
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggac tacaaggatg acgatgacaa ggattacaaa gacgacgatg ataaggacta
     2341 taaggatgat gacgacaaag aattcatggc ggacgcctgg gaagagatta ggcggttggc
     2401 ggccgacttc cagcgggcgc agttcgccga ggccacgcag aggttgtccg agcggaactg
     2461 cattgagatt gttaataaat tgattgctca gaaacagcta gaagtagttc atacactcga
     2521 tggaaaggaa tatattactc cagcccaaat tagtaaagaa atgagagatg agctacatgt
     2581 ccgaggtggt cgagtaaaca ttgttgatct acaacaggta attaatgtgg acctgattca
     2641 tattgaaaat agaattggtg acattattaa atcagaaaag catgttcagt tagtgttggg
     2701 acaactgata gatgagaatt atttggatcg gttggcagaa gaggtcaatg ataaattgca
     2761 agaaagtggt caggtcacca tatcagaact gtgtaaaact tatgatcttc ctgggaactt
     2821 tctgacacag gcactaactc agcgacttgg tagaattatc agtggacata ttgatcttga
     2881 taatagagga gtaattttta cggaagcttt tgtagctcga cataaagcac gtatccgtgg
     2941 actattcagt gctattaccc ggcctacagc tgtgaattct ttgatttcaa aatatggatt
     3001 tcaggagcag cttctttact ctgtgcttga ggaacttgtt aatagcggac gcttacgagg
     3061 cactgtggtt ggtgggagac aggataaagc tgtgtttgtc cctgacatct actccaggac
     3121 acagagtact tgggtggatt cctttttcag gcagaatggc tatctagaat ttgatgcttt
     3181 gtccagactt ggaatcccag atgctgtaag ctacataaag aaaagatata agactacaca
     3241 actcttgttt ttgaaagcag cttgtgttgg tcaaggactt gtggatcaag tggaagcatc
     3301 agtagaagaa gccatcagct ctggaacatg ggttgatatt gcacctctgc tacccacttc
     3361 tttatcagtt gaagatgctg ccatattgct tcagcaggtg atgagggcat tcagcaaaca
     3421 ggcctcaact gtagtcttta gcgacactgt tgtagtcagt gaaaaattta taaatgactg
     3481 tacagaactg ttccgtgagc tgatgcacca gaaagctgaa aaggaaatga aaaataatcc
     3541 tgtgcattta atcactgaag aagatctgaa acaaatctcc actttagaaa gcgttagtac
     3601 aagtaaaaag gataaaaaag atgagcgaag aaggaaagca acagagggca gtggaagcat
     3661 gagaggagga ggtgggggca atgccagaga gtacaaaatt aaaaaagtca agaagaaagg
     3721 aagaaaagat gatgatagtg atgatgaatc tcaatcatcc cacactggaa agaagaagcc
     3781 agagatcagt tttatgttcc aggatgagat tgaagatttt ttaagaaaac acatacaaga
     3841 tgcccctgag gagtttattt cggaacttgc tgagtactta ataaaacctc ttaataaaac
     3901 ttatctcgag gtggtacgtt cagtattcat gtcttcaaca acttctgctt ctgggacggg
     3961 cagaaaacgc acaatcaagg acttgcaaga agaagtttca aacctgtaca ataacattag
     4021 gttatttgaa aaagggatga agttttttgc agatgacaca caggctgctc ttaccaaaca
     4081 cttgctgaag tcagtgtgta ctgatatcac taacctcatt ttcaacttct tagcttcgga
     4141 tttaatgatg gcagtagacg atcctgcagc cattacaagt gaaataagaa agaaaatttt
     4201 aagtaaatta tcagaagaaa ccaaagtagc tcttacaaaa ctccataact ctctgaatga
     4261 aaagagcata gaagacttta tttcttgtct ggattctgca gcagaagctt gtgatattat
     4321 ggtgaaaagg ggagacaaaa aaagggaaag acagatactg ttccaacatc gacaagcact
     4381 ggctgaacag ctaaaggtca cagaagaccc tgctcttatt ctgcacctca catcagtcct
     4441 gttgtttcag ttttcaaccc acagcatgct ccatgcacct ggaagatgtg tcccacagat
     4501 cattgctttt cttaatagta aaattccaga ggatcagcat gctcttttgg taaagtatca
     4561 aggtttggtt gtaaagcagc tagtcagtca aagtaagaag actgggcagg gagattatcc
     4621 cttgaataat gaattagaca aagaacaaga agatgttgcc agtactactc gtaaagagct
     4681 tcaagaactt tcttcatcca ttaaagacct tgttctcaaa tctaggaaat catctgtgac
     4741 ggaagagtaa gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     4801 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     4861 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4921 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4981 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     5041 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     5101 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     5161 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     5221 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     5281 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct
     5341 gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct
     5401 ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac
     5461 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta
     5521 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg
     5581 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt
     5641 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt
     5701 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca
     5761 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg
     5821 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca
     5881 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca
     5941 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt
     6001 cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc
     6061 caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     6121 cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6181 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6241 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6301 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6361 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6421 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6481 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6541 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6601 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6661 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6721 tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6781 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6841 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6901 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6961 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7021 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7081 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7141 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7201 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7261 gcgtctccgc cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt
     7321 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7381 ggggggactg gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac
     7441 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7501 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7561 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7621 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7681 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7741 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7801 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7981 agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8041 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8101 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8161 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8221 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8281 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8821 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9301 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10081 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10141 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10201 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10261 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10321 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
    10381 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
    10441 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
    10501 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
    10561 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
    10621 agtgccaagc tg
//
