LOCUS       V023960                12521 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023960
VERSION     V023960
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6564
                     /label="UACA(NM_018003)"
                     /note="UACA(NM_018003)"
                     /gene="UACA"
     CDS             6571..6636
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6680..6891
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6904..7172
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7201..7210
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7231..7893
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7963..8016
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8017..8616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8617..9204
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9278..9511
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9583..9704
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9723..9858
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9896..9912)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9920..9936
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9944..9974)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9989..10010
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10298..10886)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11057..11917)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11918..12022)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12496..12512
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agagcctcaa gtcccgcctg
     2341 aggaggcagg acgtgcccgg ccccgcgtcg tctggcgccg ccgccgccag cgcgcatgca
     2401 gcagattgga ataaatatga tgaccgattg atgaaagcag cagaaagggg ggatgtagaa
     2461 aaagtgacct caatccttgc taaaaagggg gtcaatccag gcaaactaga tgtggaaggc
     2521 agatctgtct tccatgttgt gacctcaaag gggaatcttg agtgtttgaa tgccatcctt
     2581 atacatggag ttgatattac aaccagtgac actgcaggga gaaatgctct tcacctggct
     2641 gctaagtatg gacatgcatt gtgcctacaa aaacttctac agtacaattg tcccactgag
     2701 catgcagacc tgcagggaag aactgcactt cacgatgccg caatggcaga ttgtccttct
     2761 agcatacagc tgctttgtga ccatggggcc tctgtgaatg ccaaagatgt agacgggcgg
     2821 acaccacttg ttctggctac tcagatgagt aggccaacaa tatgtcaact gctgatagat
     2881 agaggagcgg atgttaattc cagagacaaa caaaacagaa ctgccctcat gctaggttgc
     2941 gaatatggtt gcagagatgc agtagaagtc ttaattaaaa atggtgctga tataagcttg
     3001 ctggatgcgc ttggccatga tagttcttac tatgcaagaa ttggtgacaa tctggacatt
     3061 ctaaccttgt tgaagactgc atcggaaaat accaacaaag ggagagaact ttggaagaaa
     3121 gggccatctt tgcaacagcg aaatttgaca cacatgcaag atgaagtaaa tgtgaagtca
     3181 catcagaggg agcatcaaaa tattcaggat ttggagattg aaaatgaaga tttgaaagag
     3241 aggttgagaa aaattcagca agaacaaaga atacttttgg ataaagtcaa tggtttacag
     3301 ttacagctga atgaggaagt tatggttgct gatgatctgg aaagcgagag agaaaagctg
     3361 aagtcccttt tggcagctaa agaaaagcaa catgaagaaa gcttaaggac tattgaggct
     3421 ctgaaaaata gatttaaata ttttgagagt gatcatttag gatcaggaag tcatttcagt
     3481 aaccgaaaag aagatatgct tcttaaacaa ggtcagatgt atatggcaga ctcacagtgt
     3541 acttccccag gtataccagc ccatatgcaa agcagatcta tgttaagacc tctggaacta
     3601 tctttaccca gtcaaacgtc atactctgaa aatgaaattt taaagaaaga gttagaagca
     3661 atgcgaactt tctgtgagtc agcaaaacaa gaccgactga agctccaaaa tgaactggca
     3721 cacaaagtgg cagaatgcaa agctttagca ttagaatgtg aaagggtcaa ggaggattca
     3781 gatgaacaga taaagcaatt agaagatgca ttaaaagatg tgcagaagag gatgtatgag
     3841 tcagaaggta aagttaaaca aatgcagacc cattttcttg cccttaaaga acacttaaca
     3901 agtgaagcag cctcagggaa tcacagacta accgaggaac tgaaggatca gttgaaagac
     3961 ttgaaagtaa aatatgaagg tgcttcagca gaagtgggga aattaagaaa ccaaatcaaa
     4021 caaaatgaga tgatagtaga agagtttaag agggatgaag gcaagctgat agaagaaaat
     4081 aagcgattac agaaggaact tagtatgtgt gaaatggagc gagagaagaa aggaagaaag
     4141 gtcacagaga tggaaggcca ggcaaaagaa ttgtcagcga agttggccct ttccattcca
     4201 gctgaaaaat ttgaaaacat gaagagctca ttatcaaatg aagtgaatga gaaagcaaaa
     4261 aaattagtag aaatggaaag agaacatgaa aaatcactta gtgaaattag acagttaaag
     4321 agagaacttg agaatgttaa ggccaagctt gctcagcacg tcaaaccaga ggaacatgaa
     4381 caggttaaga gcagattaga acagaaatca ggagaacttg ggaagaagat cactgagtta
     4441 acattgaaaa atcagacact acaaaaggaa attgaaaaag tttatttgga taataagctc
     4501 ctcaaggagc aagcacataa cttaacaatt gaaatgaaaa atcattatgt tcctttaaaa
     4561 gtaagtgaag acatgaaaaa gtcacatgat gcaattattg atgatcttaa tagaaagctt
     4621 ttagatgtaa cacaaaaata tacagaaaag aagttggaaa tggagaaatt gctactggaa
     4681 aatgacagct taagtaagga tgtaagccgc ctagaaactg tgtttgtacc tcctgagaaa
     4741 catgaaaaag agataatagc tctgaaatcc aatattgttg aacttaagaa acagctgtct
     4801 gaacttaaga aaaaatgtgg tgaagaccag gagaaaatac acgctctcac atctgaaaac
     4861 actaacttga agaagatgat gagtaatcag tatgtgccag ttaaaaccca tgaagaggtt
     4921 aaaatgacac tgaatgacac gttagccaaa actaacagag aattattaga tgtgaagaaa
     4981 aaatttgaag atataaatca ggaatttgta aaaataaaag ataagaatga aatattaaaa
     5041 agaaacctgg aaaacactca gaaccaaata aaagctgagt acatcagcct ggcagagcac
     5101 gaggcaaaga tgagctcgct aagtcagagc atgagaaagg tgcaggatag taatgctgaa
     5161 atcttggcca actacagaaa aggccaagaa gagattgtga cactgcatgc cgaaattaaa
     5221 gcccagaaga aggagctcga cacaatacaa gaatgcatta aggtaaaata cgccccaatt
     5281 gtcagctttg aggagtgcga gagaaaattt aaagcaacag agaaagaact aaaagaccag
     5341 ttatcagagc agacacaaaa gtatagtgtc agtgaagaag aagtcaagaa aaacaagcaa
     5401 gagaatgaca agttaaagaa ggagattttt acccttcaga aagatttgag agataagaca
     5461 gttctcattg agaagtctca tgaaatggaa agagcattaa gcagaaaaac agacgagcta
     5521 aacaaacagt taaaagactt gtcacagaaa tacacggaag taaagaatgt gaaagagaag
     5581 ctagtagaag aaaatgccaa acagacttct gagatacttg cagtgcaaaa tcttttgcaa
     5641 aaacaacatg ttccattgga acaggttgag gctctgaaaa aatctcttaa tggcacaatt
     5701 gaaaatctaa aggaagaact gaagagtatg caaaggtgtt acgagaaaga gcagcagaca
     5761 gtgaccaaac tgcatcaatt gttggagaat caaaagaact cttctgtacc cctggcagag
     5821 catttgcaga ttaaagaagc atttgagaaa gaagttggaa tcataaaagc cagcttgaga
     5881 gaaaaggaag aagaaagcca aaacaaaatg gaagaagtct ccaaacttca gtcggaggtt
     5941 cagaatacta aacaagcatt aaaaaaatta gagactagag aggtagttga cttgtctaaa
     6001 tataaagcaa caaaaagtga tttggagaca cagatttcta gcttaaatga aaaattggcc
     6061 aatctgaata gaaagtatga ggaagtatgt gaggaagttt tgcatgccaa aaagaaggaa
     6121 atatctgcaa aagatgagaa ggaattactg catttcagca ttgagcaaga aattaaggat
     6181 cagaaggaac gatgtgataa gtccttaaca acaatcacag agttacaaag aagaatacaa
     6241 gaatctgcta aacaaataga agcaaaagat aataagataa ctgaactgct taatgatgtg
     6301 gaaagattaa aacaggcact caatggcctt tcccaactca cctacacaag tgggaacccc
     6361 accaagaggc agagccagct gattgacact ctgcagcacc aagtgaaatc tctggagcaa
     6421 cagctggccg atgctgacag acagcaccaa gaagtaattg caatttatcg gacacacctt
     6481 cttagtgctg cacagggtca catggatgaa gatgttcagg aggctctgct ccagatcata
     6541 caaatgcggc aggggcttgt gtgcggatcc gactacaaag accatgacgg tgattataaa
     6601 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     6661 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6721 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6781 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6841 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     6901 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     6961 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     7021 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     7081 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     7141 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     7201 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     7261 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     7321 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     7381 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     7441 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     7501 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     7561 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     7621 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     7681 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     7741 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     7801 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     7861 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     7921 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     7981 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     8041 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     8101 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     8161 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8221 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8281 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8341 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8401 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     8461 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     8521 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     8581 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     8641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     8701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     8761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     8821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9001 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     9061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     9121 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg
     9181 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     9241 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9301 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9361 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9421 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9481 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9541 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     9601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     9721 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9781 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9841 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     9901 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9961 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10021 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10081 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10201 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10261 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10321 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10741 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10801 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10861 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10921 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10981 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11041 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11101 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11161 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11221 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11281 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11341 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11401 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11461 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11521 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11581 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11641 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11701 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11761 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11821 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11881 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11941 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    12061 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    12121 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    12181 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    12241 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    12301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    12361 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    12421 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    12481 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
