LOCUS       V024324                10733 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024324
VERSION     V024324
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4776
                     /label="Txndc16(NM_001310534)"
                     /note="Txndc16(NM_001310534)"
                     /gene="Txndc16"
     CDS             4783..4848
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4892..5103
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5116..5384
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5413..5422
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5443..6105
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6175..6228
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6229..6828
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6829..7416
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7490..7723
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7795..7916
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7935..8070
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8108..8124)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8132..8148
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8156..8186)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8201..8222
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8510..9098)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9269..10129)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10130..10234)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10708..10724
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tgtcttctgg cttcagtgtc
     2341 tttagagctg gtgttgcatt ggttttgatg tgcagttttt acaagtcaac tgaagactct
     2401 ctcccagaac tgactcctca gcaatatttc agcacactgc agccaggaaa agcctcatta
     2461 gtttatttct gccaagttgg ttctctaagt aactctgtgt ttcttgaaga actaaaagaa
     2521 gctgtcaagc ctctccagga ctatgggatt tcagttgcaa aggttacttg tgttgaagaa
     2581 gaagcgtcaa ggtactgtgg ggaagaagag ggtttgatga aagcgtattt attcagaggc
     2641 aacatacttc tcagagaatt ccccacggac atcttgtttg atgtgaacgc catcattgct
     2701 catgttctct ttgctctgct ttttaatgaa gtaaaatata ttaccaccct ggaagacctt
     2761 cacagcatag aaaactctct gaaaggaaaa tcaaatatga tattctcata cgtagaagcc
     2821 atcggaacac cagagcatag agcagtcatg gaagctgctt ttgtgtatgg gacctcttac
     2881 cagtttgcct taaccacaga aattgctctt ttagaaaata tcggctctga gagtatagaa
     2941 catgcacatc tctacttttt tcattgtaaa ctggtcttgg acttgaccga gcattgcaga
     3001 agaacactaa tggaacagcc attgaccaca ctcaatattc acgtatttgt gaagacaatg
     3061 aacgcacctc tgttgatgga agtggctgaa gatccccagc aggtctcaac cgttcatcta
     3121 caactaggat tgccactggt ttttattatc agccagcgcg ctacccagga ggctgacaga
     3181 agaactgcgg agtgggtggc gtggcacctt ctggggaaag caggggttct gctcttgttg
     3241 agggactcca tggatgttaa cattcctcag cacgctaatg tggccttcag aagagcagag
     3301 aaggatgtgc ctgtggagtt tctggtgttg aatgatgttg agttaatcat ttcccacgtg
     3361 aaaaataata tgcacattga agaaatacaa gaagatgaag gcgaagacat ggagggccca
     3421 gatctggctg ttgaagatga tgaggtggca ggaactgttt acagagatag gaagaaacca
     3481 ctacctttgg agttgtctgt ggagctaaca gaggagactt ttaatacaac ggtcatgact
     3541 tctgacagca tagtgctctt ctatgctacc tggcatgcag tatccatggc atttctacaa
     3601 tcctatattg atgtggcaat taagttgaaa ggcaggtcca ccatccttct caccagaata
     3661 aattgtgcag attggtctga tatatgtacg aagcaaaatg ttactgcatt tcccgtggta
     3721 aagttgtata aggaaggcga gagcccagtg tcttatgctg gtatgttggc aactaaagat
     3781 ctcctgaaat ttatccagtt gaataagatc tcctgtccag tgaacatagc atctatccag
     3841 gaagcagaaa aatacctgcg tggggaatta tataaagacc tgcccagctc tgctagtgtg
     3901 tcagtactgg ggctcttcag cccagccatg gcatcagcaa aagaactttt cagggaagca
     3961 ggaaagcagc tgagaggctc tgtcattacg ggaatttatt ctgaagatga tgtgtggata
     4021 ctgtcaaata aatatgctac aactctccca gccctgctac tggccaggcc taaagaaggt
     4081 agaatagaga gtgtcccact agacaccacc ctggtacaag acatggctca gatactagca
     4141 aatgcactac tggaagcatt tccagaaatc actgtggaaa atcttcccac atatttaaga
     4201 tttcagagac cgctacttct cttgtttagt ggtggcagca taaaccctca gtacaggaac
     4261 acgatcctgg cactggtgag acagaagcag ctggattcgt ttaccccttg ctggttaaat
     4321 ctgaagaaca ctcccgtggg gaggggcatc ttgaaggcat attttggccg tctgccacca
     4381 cttcctcagc tgctgttggt gaatctgcat tcgggtggcc aagtgtatgc atttccttca
     4441 tcccagtctg tgacggaaca aagccttgtg ttgtggctga aacatttaca agcgggacta
     4501 gaaaatccca tcacagtctt gtctgcccag gaatggaaac ctcctcttcc tgcctttgat
     4561 tttctgaata tgatggacgc tcctacatcc caagctccca cgaagaaagt tctagagtgt
     4621 cagaaagaag cagaggtgca ggagagtgct gagctgcagc ctggagacag atcaacagcc
     4681 aggagagagc cggtcgaaat gctgagaata aagcgctgga acactgccaa ctggcccaaa
     4741 gacacacagg agccttttca ccacgacaag gagttaggat ccgactacaa agaccatgac
     4801 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     4861 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4921 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4981 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     5041 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     5101 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     5161 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5221 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5281 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5341 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5401 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5461 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5521 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5581 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5641 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5701 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5761 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5821 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5881 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5941 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     6001 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     6061 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     6121 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     6181 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6241 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6301 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6361 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6421 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6481 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6541 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6601 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6661 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6721 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6781 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6841 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6901 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6961 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7021 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7081 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7141 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7201 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7261 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7321 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     7381 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     7441 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7501 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7561 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7621 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7681 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7741 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7921 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8041 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     8101 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8161 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8221 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8281 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8341 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8401 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8941 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9121 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9181 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9241 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9301 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9361 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9421 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9481 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9541 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9601 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9661 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9721 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9781 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9841 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9901 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9961 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10021 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10081 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10141 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10201 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10261 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10321 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10381 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10441 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10501 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10561 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10621 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10681 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
