LOCUS       V022590                10806 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022590
VERSION     V022590
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4915
                     /label="Trpv4(NM_023970)"
                     /note="Trpv4(NM_023970)"
                     /gene="Trpv4"
     promoter        4965..5176
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5189..5457
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5486..5495
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5516..6178
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6248..6301
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6302..6901
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6902..7489
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7563..7796
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7868..7989
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8008..8143
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8181..8197)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8205..8221
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8229..8259)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8274..8295
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8583..9171)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9342..10202)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10203..10307)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10781..10797
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggcaga tcctggtgat ggcccccgtg cagcgcctgg
     2341 ggatgtggct gagccccctg gagacgagag tggcacttct ggtggggagg ccttccccct
     2401 ctcttccctg gccaacctgt ttgagggaga ggaaggctcc tcttctcttt caccagtgga
     2461 tgctagccgc cctgctggcc ccggggatgg acgtccaaac ctgcgtatga agttccaggg
     2521 cgctttccgc aagggggttc ccaaccccat tgacctgctg gagtccaccc tgtatgagtc
     2581 ctcagtagtg cctgggccca agaaagcgcc catggattcg ttgttcgact atggcactta
     2641 ccggcaccac cccagtgaca acaagagatg gaggaggaag gtcgtagaga agcagccaca
     2701 gagccccaaa gctcccgccc cccagccacc ccccatcctc aaagtcttca accggcccat
     2761 cctctttgac atcgtgtccc ggggctccac tgccgacctg gacggactgc tctcctactt
     2821 gctgacccac aagaagcgcc tgactgatga ggagttccgg gaaccatcca cagggaagac
     2881 ctgcctgccc aaggcacttc tgaacttaag caatggccga aacgacacca tcccagtgtt
     2941 gctggacatt gcggaacgca cgggcaacat gcgggagttc atcaactcgc ccttcagaga
     3001 catctactac cgagggcaga cggcactgca catcgccatt gaacggcgct gcaagcatta
     3061 cgtggagctc ctggtggccc agggagccga tgtgcacgcg caggcccgag ggcggttctt
     3121 ccagcccaag gatgagggtg gctacttcta ctttggggag ctgcccttgt ccttggcagc
     3181 ctgcaccaac cagccgcaca tcgtcaacta cctgacagag aaccctcaca agaaagccga
     3241 tatgaggcga caggactcca gaggcaacac ggtgctccac gcgctggtgg ccatcgctga
     3301 caacacccga gagaacacca agtttgtcac caagatgtat gacctgttgc ttctcaagtg
     3361 ctcccgcctc ttcccagaca gcaacctgga gactgtgctt aacaatgacg gtctttcgcc
     3421 cctcatgatg gctgccaaga ctggcaagat cggggtcttt cagcacatca tccgacggga
     3481 ggtgacagat gaggacacac ggcacctgtc tcgcaagttc aaggactggg cctacgggcc
     3541 tgtgtattct tctctctacg acctctcctc cctggatacg tgcggggagg aagtgtccgt
     3601 gctggagatc ctggtttaca acagcaagat cgagaaccgc catgagatgc tggctgtgga
     3661 gcccattaac gaactgctga gggacaagtg gcgtaagttc ggggccgtgt ccttctacat
     3721 caacgttgtc tcctatctgt gtgccatggt catcttcacc ctcacagcct actatcagcc
     3781 actggagggc acgccaccct acccttaccg taccacggtg gactacctga ggctggctgg
     3841 tgaggtcatc acgctcctca caggagtcct gttcttcttt accagtatca aagacttgtt
     3901 catgaagaaa tgccctggag tgaattctct cttcgtcgat ggctccttcc agttgctcta
     3961 cttcatctac tcagtgctgg tggttgtgtc tgcggcgctc tacctggcag ggatcgaggc
     4021 ctatctggct gtgatggtct ttgccctggt cctgggctgg atgaatgccc tttacttcac
     4081 ccgtgggctg aagctgacag ggacctacag catcatgatt cagaagatcc tcttcaaaga
     4141 tctcttccgc tttctgctgg tctacctgct ttttatgatt ggctatgcct cagctctggt
     4201 caccctcctg aatccgtgca ccaacatgaa ggtctgtaac gaggaccaga gcaactgcac
     4261 ggtgccctca taccccgcgt gccgggacag cgagaccttc agcgccttcc tactggacct
     4321 cttcaagctc accatcggca tgggcgacct ggagatgctg agcagcgcta agtaccccgt
     4381 ggtcttcatt ctcctgctgg ttacctacat catcctcacc ttcgtgctcc tgctgaacat
     4441 gctcatcgcc ctcatgggtg agaccgtggg ccaggtgtcc aaggagagca agcacatctg
     4501 gaagctgcag tgggccacca ccatcctgga catcgagcgc tccttccctg tgttcctgag
     4561 gaaggccttc cgctccggag agatggtgac agtgggcaag agctcggatg gcactccaga
     4621 ccgcaggtgg tgcttcaggg tggacgaggt gaactggtct cactggaacc agaacctggg
     4681 catcattaac gaggaccccg gcaagagcga gatctaccag tactatggct tctcccatac
     4741 catggggcgc ctccgcaggg atcgctggtc ctcagtggtg ccccgcgtgg tggagctgaa
     4801 caagaactca ggcacagatg aagtggtggt ccccctggat aacctaggga accccaactg
     4861 tgacggccac cagcaaggtt atgctcccaa gtggagggcg gaggacgcac cactgtagga
     4921 tccgcggccg cgaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
     4981 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
     5041 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     5101 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     5161 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg
     5221 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg
     5281 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct
     5341 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac
     5401 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa
     5461 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc
     5521 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg
     5581 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg
     5641 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt
     5701 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta
     5761 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta
     5821 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga
     5881 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca
     5941 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg
     6001 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag
     6061 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa
     6121 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag
     6181 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg
     6241 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     6301 tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6361 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6421 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6481 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6541 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6601 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6661 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6721 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6781 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6841 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6901 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7021 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7081 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7141 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7201 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7261 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     7321 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7381 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7441 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt
     7501 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7561 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     7621 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7681 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7741 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7801 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7861 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7981 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     8041 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8101 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     8161 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10321 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    10381 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
    10441 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
    10501 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
    10561 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10621 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    10681 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    10741 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    10801 aagctg
//
