LOCUS       V046906                10792 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046906
VERSION     V046906
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4818
                     /label="Tcirg1(NM_016921)"
                     /note="Tcirg1(NM_016921)"
                     /gene="Tcirg1"
     CDS             4825..4890
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4934..5145
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5158..5426
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5455..5464
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5485..6147
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6217..6270
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6271..6870
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6871..7459
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7533..7766
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7838..7959
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7999..8134
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8167..8183)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8191..8207
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8215..8245)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8260..8281
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8569..9157)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9328..10188)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10189..10293)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10767..10783
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gctctatgtt ccggagtgaa
     2341 gaggtggcct tggtccagct cctgttgccc acagggtctg cttacaactg tgtgagccag
     2401 cttggtgagc tgggcctcgt ggagttcaga gacctcaacg aatccgtgag cgccttccag
     2461 agacgcttcg tggtagacgt ccggcgctgc gaggaactgg agaagacgtt taccttcttg
     2521 cgggaagaag tgcagcgggc aggtctgacg ctggccccac ctgaggggac actgccagca
     2581 ccacctcccc gagacctgct gcgcatccag gaggaaacgg atcgcttggc ccaggagctt
     2641 cgggatgttc gtggcaatca gcaggcactg cgggcccagc tgcaccagct gcggcttcac
     2701 tcggctgtgc tgggccagag ccacagcccg ccggtggcag ctgaccatac tgaaggaccc
     2761 ttctctgaga caacacccct gcttccaggc acccgggggc cacattcaga cctgaaggtc
     2821 aattttgtgg caggtgctgt ggagccctac aaggctgcgg ccctggagcg cctgctgtgg
     2881 agagcttgcc gcggcttcct cattgccagc tttcgggaga ctgaggggca gctggaggac
     2941 ccagtgacgg gtgagcctgc aacttggatg acctttgtca tctcctactg gggtgagcag
     3001 atcggacaga agatccgaaa gatcacagac tgcttccact gtcatgtctt cccatacctg
     3061 gagcaggagg aagcccggtt caggaccttg cagcagctgc agcagcaaag ccaggagctt
     3121 caggaagtcc tgggggagac agaccggttc ctgagccagg tgttgggccg ggtgcagcag
     3181 ctgctgcccc catggcaggt gcagatccac aagatgaagg cagtgtacct gaccctcaac
     3241 cagtgcagtg tgaacaccac acacaagtgc ctcatcgcgg aggtctggtg tgccgcgagg
     3301 gacctgccca ctgtgcagca ggcgctgcag agcggctcaa gtgaggaagg agtgagtgct
     3361 gtcgctcacc gcatcccctg ccaggacatg cctccaaccc tcatcaggac caaccgcttc
     3421 acctccagct tccagggcat tgtggatgcc tatggtgtgg gccgctacag ggaagttaac
     3481 cctgctccct acaccatcat cacattcccc tttctcttcg ctgtgatgtt tggcgatgtg
     3541 gggcacggac tgctcatgtt tctctttgcc ctggccatgg tcctcactga aaaccgtcca
     3601 gctgtgaagg ctgcacaaaa cgagatctgg cagaccttct ttgggggtcg atacctactc
     3661 ctgctcatgg gtctgttctc cgtctacact ggcttcatct acaatgagtg cttcagccga
     3721 gccaccacta ttttcccctc aggttggagt gtggctgcca tggccaacca gtcaggctgg
     3781 agtgacgagt atctgtccca gcactccatg ctcaccctga accctaacat cactggtgtc
     3841 ttccttggac catatccctt tggcattgac ccgatctgga gcctggccac gaaccacctg
     3901 agctttctca actccttcaa gatgaagatg tctgtcatcc ttggggtcac gcacatggcc
     3961 tttggggtgt tcctcagcat cttcaaccac gtgcactttg gccaggccca ccggctactg
     4021 ctggagacct tgcctgagct catcttcctg ctgggtctct ttggctacct tgtgttcctc
     4081 attgtctaca agtgggtgaa tgtctcagct gctagcgcct cctcggcccc cagtatcctc
     4141 attcacttca tcaatatgtt cctcttctct caaaacccca ccaatcatct gctcttccat
     4201 gggcaggaag tggtacagta cgtgctggtg gtcctggctt tggctaccgt tcctatcctg
     4261 ttgctgggca cacccttgta cctgctgcgc cagcaccgcc acagaagaaa cactcagaga
     4321 aggccagcag gccagcagga tgaggacacg gacaagcttc tggcctcccc tgatgcatcc
     4381 accttggaga acagctggag ccctgatgag gagaaggctg ggagcccagg ggatgaagaa
     4441 actgagtttg tcccttctga gatcttcatg caccaagcaa tccacaccat tgagttctgc
     4501 ctgggctgca tctccaacac agcctcctac ttgcgtctct gggccctgag cctggcccat
     4561 gcccagctgt ctgaggtcct gtgggccatg gtgatgcgca taggcctggg catgggccga
     4621 gagatcggcg tggcagctgt ggtgctggtc cctgtgtttg ctgcctttgc tgtgttgact
     4681 gtggccatcc tgttagtgat ggaggggctc tcagccttcc tgcatgccct gcggttgcac
     4741 tgggtggagt tccagaacaa gttctattca ggcaccggct acaagctcag ccccttcacc
     4801 ttcactgtgg acagtgacgg atccgactac aaagaccatg acggtgatta taaagatcat
     4861 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     4921 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4981 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5041 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5101 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5161 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5221 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5281 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5341 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5401 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5461 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5521 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5581 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5641 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5701 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5761 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5821 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     5881 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     5941 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     6001 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6061 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6121 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6181 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6241 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6301 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6361 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6421 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6481 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6541 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6601 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6661 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6721 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6781 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6841 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     6901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7261 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7321 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7381 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7441 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     7501 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7561 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7621 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7681 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7741 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7801 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7981 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8041 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8101 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     8161 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8221 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8341 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8401 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8461 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8521 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8581 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8641 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8701 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8761 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8821 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8881 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8941 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9001 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9061 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9181 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9241 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10321 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10381 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10441 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10501 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10561 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10621 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10681 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10741 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
