LOCUS       V038864                10456 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038864
VERSION     V038864
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4482
                     /label="Tars1(NM_033074)"
                     /note="Tars1(NM_033074)"
                     /gene="Tars1"
     CDS             4489..4554
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4598..4809
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4822..5090
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5119..5128
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5149..5811
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5881..5934
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5935..6534
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6535..7123
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7197..7430
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7502..7623
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7663..7798
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7831..7847)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7855..7871
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7879..7909)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7924..7945
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8992..9852)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9853..9957)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10431..10447
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgcaggagaa ggctagcagc
     2341 ccttcgggga agatggacgg cgagaagccg gtggatgcca gcgaggagaa gcggaaagaa
     2401 ggaggcaaga agaagagcaa agatggaggt ggagatggag gtcgagcaga gttgaatccc
     2461 tggcctgaat acattaacac acgccttgat atgtacaaca aactcaaagc agaacacgat
     2521 tctatcctgg ccgaaaaggc agcaaaagat agcaagccga ttaaagtcac cctgcctgac
     2581 ggcaagcagg tggatgcgga gtcctggaaa accacgccgt atcagattgc ttgtggaatt
     2641 agtcagggcc tggctgacaa caccgttgtt gctaaggtga acaaagtcgt ttgggacctg
     2701 gaccgcccgc tggagacaga ttgcacgttg gagctgctca agtttgagga cgaggaagcg
     2761 caggccgtat actggcactc cagtgcccac ataatgggcg aggccatgga gagagtctat
     2821 ggcggatgtc tatgttacgg cccaccaata gaaaacggct tctattatga tatgtacctt
     2881 gaagaagggg gcgtgtccag caatgacttc tcttccctgg aaactttgtg caagaaaatc
     2941 attaaagaga aacaaacttt cgaaagattg gaagttaaaa aggaaacatt actagaaatg
     3001 tttaagtaca acaagttcaa gtgccggata ttgaatgaga aagtgaatac tccgaccacg
     3061 actgtctaca gatgtggccc tctgatagat ctctgccggg gtcctcatgt ccgacacacc
     3121 ggcaagatta agactttgaa gatacacaag aattcttcca cgtactggga aggcaaagca
     3181 gacatggaga cgctccagcg gatctatggc atttcattcc ctgaccccaa gctactgaaa
     3241 gagtgggaga agttccaaga ggaagccaaa aaccgagacc acaggaaaat cgggagggac
     3301 caagaactat atttcttcca tgaacttagc cctggaagtt gctttttcct gcccaaagga
     3361 gcctacattt ataatacact aatggaattt atcaggagtg aatataggaa aagagggttc
     3421 caggaggtcg tcactcccaa catcttcaat agccggctct ggatgacctc tggccactgg
     3481 cagcactaca gcgagaacat gttctccttt gaggtggaga aggagcagtt tgcacttaag
     3541 cccatgaact gcccgggaca ctgcctgatg ttcgatcacc ggccacggtc ctggcgagag
     3601 ctgcccctgc ggctagctga ctttggtgtg ctgcatagga atgagctctc gggggctctc
     3661 accgggctca cacgggtccg aagattccag caggatgacg cacacatctt ctgtgccatg
     3721 gagcagattg aagatgaaat caaaggttgt ttggattttc ttcgcacagt atatagtgtc
     3781 tttggatttt catttaaatt gaatctttct actcgcccag aaaaattcct tggagatatt
     3841 gaaatatgga accaagctga gaaacaactt gaaaacagtt tgaatgagtt cggtgagaag
     3901 tgggaactaa atcctggaga tggagccttc tatgggccaa agattgacat acagataaaa
     3961 gatgccatcg gtcgctacca ccagtgtgca accatccagc tggactttca gttgcccatc
     4021 aggtttaacc ttacttacgt cagccacgat ggcgatgata agaaaaggcc agtgattgtt
     4081 cacagagcca tcctggggtc ggtggaaaga atgattgcca tcctcacaga aaactatggg
     4141 ggcaaatggc ctttctggct ctctcctcgc caggtgatgg tggtcccagt gggaccgaca
     4201 tgtgatgaat atgcccaaaa ggtacggcaa caattccatg atgctaaatt catggcggac
     4261 actgacctgg atccaggctg taccttgaat aagaagatca gaaacgcaca gttagcacag
     4321 tataacttca tccttgttgt tggtgaaaaa gagaaagcca gtggcacggt gaacatccgc
     4381 acgagagaca ataaggtcca cggagagcgg acagtggagg agacggtgcg gcggctgcag
     4441 cagctcaagc agacgcgcag caagcaggca gaggaggagt tcggatccga ctacaaagac
     4501 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     4561 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4621 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     4681 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4741 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4801 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     4861 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     4921 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     4981 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5041 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5101 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5161 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5221 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     5281 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     5341 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     5401 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     5461 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     5521 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     5581 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     5641 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     5701 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     5761 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     5821 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     5881 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     5941 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6001 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6061 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6121 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6181 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6241 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6301 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6361 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6421 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6481 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     6541 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6601 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6661 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6721 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6781 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6841 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6901 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6961 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7021 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7081 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7141 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7201 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7261 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7321 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7381 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7441 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7501 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7561 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7621 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7741 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7801 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     7861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9961 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10021 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10081 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10141 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10261 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10321 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10381 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10441 ggccagtgcc aagctg
//
