LOCUS       V029298                10432 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029298
VERSION     V029298
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4458
                     /label="Tagap(NM_145968)"
                     /note="Tagap(NM_145968)"
                     /gene="Tagap"
     CDS             4465..4530
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4574..4785
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4798..5066
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5095..5104
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5125..5787
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5857..5910
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5911..6510
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6511..7099
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7173..7406
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7478..7599
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7639..7774
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7807..7823)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7831..7847
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7855..7885)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7900..7921
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8209..8797)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8968..9828)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9829..9933)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10407..10423
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agctgataag cagtctcgat
     2341 ggttcaaaaa cacttaatgc taacaacatg gagacattaa ttgaatgtca gtcagagggt
     2401 gatatcaaag tgcctcccct gctgacatca tgtgagagtg aagacagcat ttgccagcta
     2461 actgaaatta agaagagaaa gaaagtgctg tcctggccat ctctcatgag aaagctctct
     2521 ccttcatccg acttctctgg gtcattggaa ccagagctga aagtgtcgct gtttgaccaa
     2581 cccttgtcga tcatctgtgg ggagaacgac acacttccca gacccatcca ggacatcctc
     2641 accatcctct gccttaaagg tccttcaacg gaagggatat tcaggaaagc tgccagcgag
     2701 aaagcccgca aggagctgaa ggaggggctt aactgtgggg tctccgtgaa tctgaagcag
     2761 ctccctgtgc acctcctagc tgtggtcttc aaggacttcc tccgagggat acccctgaag
     2821 ctactctcct gtgatctctt tgaggactgg atgggcgccc tggagaagcc caccgaggag
     2881 gacagaatcg aggccctgaa gcaggttgct ggtggcctcc cccggcccaa ccttctcctg
     2941 ctcaggcact tgctctacgt gctccacctc atcagcaaga acgctgaggt caacaagatg
     3001 gactccagca acctcgccat ctgcatcggg cctaacatgc tcactctgaa gaacgaccag
     3061 agcctgtcct tccaggccca gaaggacctg aacaataagg ttaagatctt ggtggaattc
     3121 ctcatcgaca actgctttga gatatttggg gagaacattc ggacgcgttc ccgcatcact
     3181 tctgacgact ccctggaaca cactgacagt tcagacgtgt caactctgca gaatgactcg
     3241 gcctatgaca gcaatgaccc agatgtggag cccacaagtg gcgcagcctc tcccaacagg
     3301 caactggagg gtcccactcc cacaatggct ggtctggata cccggggcca acgggacacc
     3361 tgtgagtcaa gctcagagtc cagcgttagc atggtagtca ggctgaaaag ctccattgtc
     3421 caacaagaca ggcggttctc tgaacccaac atgtcaccct cacgagagtg cctcgtgggc
     3481 ccaacatcca agcaaaagct agcaaggagt gaggacagct tcactctgtc ccaggacgcc
     3541 tcctgttctg aaggcgatga agctgaagat ccctttacag aggaagtctt cccagcagtg
     3601 gacagcaaac ccaagagacc tgtggatttg aaaataaaga attggaccca aggtttagca
     3661 tctccacagg gacacataac caaagctttc tccagatcct ccccaggcga atctttgggc
     3721 agctcacctg tgccttctcc atcctgcccc aagagaaact tcttcaccag acaccagagt
     3781 ttcacaacaa agacagacaa aaccaaaccc cagagagaaa ttagaaagca ctccatgtcg
     3841 ttttcctttg cgtctcacaa gaaagtgctg ccccgaacct ccagcattgg gtctgagaaa
     3901 tccaaagact tttctagaga ccaactccag aaggacttga ggaaagagag ccaactttct
     3961 ggcagaatcg tccaggaaaa tgagtcagaa atccaaagcc aaacatctct gggcttcagc
     4021 ttgtctggca cctgggccct ctcagtcgac aacacgttcc agttagtgga tatgaggaaa
     4081 ccaggaagcc caccatctta cgaagaggcc atttattacc agacatcagg actcacagcc
     4141 tacggtggcc agacagttgg gagtatgagg tcaagaatgt tcaagccaag cacagcagtg
     4201 ccccctgtgc cttctcacca tggaggtgac ctcagtgaag ggacacctgg tggacacaga
     4261 ttgtcttctg tgactgagca ctggacacac agtcagactg tccatgtctc tatagaaact
     4321 caggggagat ctgagctaca ccagttgagg acagtgtccg agtccatgca gaaggctaag
     4381 ctggactgtc ttgggccaca acacagccac ttagtctttg aggctgacca actctgctgt
     4441 gctagagaat cctacattgg atccgactac aaagaccatg acggtgatta taaagatcat
     4501 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     4561 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4621 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4681 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4741 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     4801 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     4861 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     4921 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     4981 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5041 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5101 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5161 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5221 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5281 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5341 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5401 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5461 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     5521 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     5581 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     5641 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     5701 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     5761 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     5821 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     5881 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     5941 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6001 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6061 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6121 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6181 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6241 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6301 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6361 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6421 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6481 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     6541 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6601 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6661 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6721 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6781 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6841 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6901 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6961 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7021 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7081 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     7141 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7201 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7381 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7441 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7501 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7561 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7741 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     7801 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8641 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9961 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    10021 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10081 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10141 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    10201 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    10261 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    10321 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    10381 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
