LOCUS       V027025                10046 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027025
VERSION     V027025
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4971
                     /label="TYRO3(NM_006293)"
                     /note="TYRO3(NM_006293)"
                     /gene="TYRO3"
     promoter        5016..5227
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5240..5508
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5535..6134
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6141..6729
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6803..7036
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7108..7229
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7248..7383
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7421..7437)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7445..7461
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7469..7499)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7514..7535
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7823..8411)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8582..9442)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9443..9547)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10021..10037
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcgctg aggcggagca tggggcggcc ggggctcccg
     2341 ccgctgccgc tgccgccgcc accgcggctc gggctgctgc tggcggctct ggcttctctg
     2401 ctgctcccgg agtccgccgc cgcaggtctg aagctcatgg gagccccggt gaagctgaca
     2461 gtgtctcagg ggcagccggt gaagctcaac tgcagtgtgg aggggatgga ggagcctgac
     2521 atccagtggg tgaaggatgg ggctgtggtc cagaacttgg accagttgta catcccagtc
     2581 agcgagcagc actggatcgg cttcctcagc ctgaagtcag tggagcgctc tgacgccggc
     2641 cggtactggt gccaggtgga ggatgggggt gaaaccgaga tctcccagcc agtgtggctc
     2701 acggtagaag gtgtgccatt tttcacagtg gagccaaaag atctggcagt gccacccaat
     2761 gcccctttcc aactgtcttg tgaggctgtg ggtccccctg aacctgttac cattgtctgg
     2821 tggagaggaa ctacgaagat cgggggaccc gctccctctc catctgtttt aaatgtaaca
     2881 ggggtgaccc agagcaccat gttttcctgt gaagctcaca acctaaaagg cctggcctct
     2941 tctcgcacag ccactgttca ccttcaagca ctgcctgcag cccccttcaa catcaccgtg
     3001 acaaagcttt ccagcagcaa cgctagtgtg gcctggatgc caggtgctga tggccgagct
     3061 ctgctacagt cctgtacagt tcaggtgaca caggccccag gaggctggga agtcctggct
     3121 gttgtggtcc ctgtgccccc ctttacctgc ctgctccggg acctggtgcc tgccaccaac
     3181 tacagcctca gggtgcgctg tgccaatgcc ttggggccct ctccctatgc tgactgggtg
     3241 ccctttcaga ccaagggtct agccccagcc agcgctcccc aaaacctcca tgccatccgc
     3301 acagattcag gcctcatctt ggagtgggaa gaagtgatcc ccgaggcccc tttggaaggc
     3361 cccctgggac cctacaaact gtcctgggtt caagacaatg gaacccagga tgagctgaca
     3421 gtggagggga ccagggccaa tttgacaggc tgggatcccc aaaaggacct gatcgtacgt
     3481 gtgtgcgtct ccaatgcagt tggctgtgga ccctggagtc agccactggt ggtctcttct
     3541 catgaccgtg caggccagca gggccctcct cacagccgca catcctgggt acctgtggtc
     3601 cttggtgtgc taacggccct ggtgacggct gctgccctgg ccctcatcct gcttcgaaag
     3661 agacggaaag agacgcggtt tgggcaagcc tttgacagtg tcatggcccg gggagagcca
     3721 gccgttcact tccgggcagc ccggtccttc aatcgagaaa ggcccgagcg catcgaggcc
     3781 acattggaca gcttgggcat cagcgatgaa ctaaaggaaa aactggagga tgtgctcatc
     3841 ccagagcagc agttcaccct gggccggatg ttgggcaaag gagagtttgg ttcagtgcgg
     3901 gaggcccagc tgaagcaaga ggatggctcc tttgtgaaag tggctgtgaa gatgctgaaa
     3961 gctgacatca ttgcctcaag cgacattgaa gagttcctca gggaagcagc ttgcatgaag
     4021 gagtttgacc atccacacgt ggccaaactt gttggggtaa gcctccggag cagggctaaa
     4081 ggccgtctcc ccatccccat ggtcatcttg cccttcatga agcatgggga cctgcatgcc
     4141 ttcctgctcg cctcccggat tggggagaac ccctttaacc tacccctcca gaccctgatc
     4201 cggttcatgg tggacattgc ctgcggcatg gagtacctga gctctcggaa cttcatccac
     4261 cgagacctgg ctgctcggaa ttgcatgctg gcagaggaca tgacagtgtg tgtggctgac
     4321 ttcggactct cccggaagat ctacagtggg gactactatc gtcaaggctg tgcctccaaa
     4381 ctgcctgtca agtggctggc cctggagagc ctggccgaca acctgtatac tgtgcagagt
     4441 gacgtgtggg cgttcggggt gaccatgtgg gagatcatga cacgtgggca gacgccatat
     4501 gctggcatcg aaaacgctga gatttacaac tacctcattg gcgggaaccg cctgaaacag
     4561 cctccggagt gtatggagga cgtgtatgat ctcatgtacc agtgctggag tgctgacccc
     4621 aagcagcgcc cgagctttac ttgtctgcga atggaactgg agaacatctt gggccagctg
     4681 tctgtgctat ctgccagcca ggacccctta tacatcaaca tcgagagagc tgaggagccc
     4741 actgcgggag gcagcctgga gctacctggc agggatcagc cctacagtgg ggctggggat
     4801 ggcagtggca tgggggcagt gggtggcact cccagtgact gtcggtacat actcaccccc
     4861 ggagggctgg ctgagcagcc agggcaggca gagcaccagc cagagagtcc cctcaatgag
     4921 acacagaggc ttttgctgct gcagcaaggg ctactgccac acagtagctg ttaggcggcc
     4981 gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     5041 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
     5101 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     5161 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     5221 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
     5281 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
     5341 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
     5401 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
     5461 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     5521 ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     5581 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     5641 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     5701 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     5761 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     5821 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     5881 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     5941 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     6001 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     6061 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     6121 agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6541 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6721 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6781 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     6841 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7021 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7081 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7201 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7261 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7321 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7381 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     7441 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7501 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7561 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7621 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7681 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7741 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8041 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8101 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8161 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8221 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8281 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8341 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8401 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8461 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8521 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8581 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8641 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8701 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8761 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8821 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8881 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8941 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9001 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9061 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9121 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9181 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9241 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9301 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9361 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9421 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9481 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9541 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9601 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9661 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9721 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9781 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9841 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     9901 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     9961 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10021 gtaaaacgac ggccagtgcc aagctg
//
