LOCUS V028998 9734 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028998 VERSION V028998 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2291..2956 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="MPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGAL TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA " /label="CopGFP" CDS 2972..4588 /label="TRIM26(NM_003449)" /note="TRIM26(NM_003449)" /gene="TRIM26" CDS 4595..4660 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4704..4915 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4928..5196 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 5223..5822 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5829..6417 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6491..6724 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6796..6917 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6936..7071 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7109..7125) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7133..7149 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7157..7187) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7202..7223 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7511..8099) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8270..9130) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9131..9235) /gene="bla" /label="AmpR promoter" primer_bind 9709..9725 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta 2281 gaacgccacc atgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt 2341 ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa 2401 gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg 2461 ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc 2521 catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct 2581 gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt 2641 gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc 2701 caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac 2761 cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa 2821 gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt 2881 ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac 2941 ccccatcgcc ttcgccggcg gtggaggcag tatggccacg tcagccccac tacggagcct 3001 ggaagaggag gtgacctgct ccatctgtct tgattacctg cgggaccctg tgaccattga 3061 ctgtggccac gtcttctgcc gcagctgcac cacagacgtc cgccccatct cagggagccg 3121 ccccgtctgc ccactctgca agaagccttt taagaaggag aacatccgac ccgtgtggca 3181 actggccagc ctggtggaga acattgagcg gctgaaggtg gacaagggca ggcagccggg 3241 agaggtgacc cgggagcagc aggatgcaaa gttgtgcgag cgacaccgag agaagctgca 3301 ctactactgt gaggacgacg ggaagctgct gtgcgtgatg tgccgggagt cccgggagca 3361 caggccccac acggccgtcc tcatggagaa ggccgcccag ccccacaggg aaaaaatcct 3421 gaaccacctg agtaccctaa ggagggacag agacaaaatt cagggcttcc aggcaaaggg 3481 agaagctgat atcctggccg cgctgaagaa gctccaggac cagaggcagt acattgtggc 3541 tgagtttgag cagggtcatc agttcctgag ggagcgggag gaacacctgc tggaacagct 3601 ggcgaagctg gagcaggagc tcacggaggg cagggagaag ttcaagagcc ggggcgtcgg 3661 ggagcttgcc cggctggccc tggtcatctc cgaactggag ggcaaggcgc agcagccagc 3721 tgcagagctc atgcaggaca cgagagactt cctaaacagg tatccacgga agaagttctg 3781 ggttgggaaa cccattgctc gagtggttaa aaaaaagacc ggagaattct cagataaact 3841 cctctctctg caacgaggcc tgagggaatt ccaggggaag ctgctgagag acttggaata 3901 taagacagtg agcgtcaccc tggacccaca gtcggccagt gggtacctgc agctgtcaga 3961 ggactggaag tgcgtgacct acaccagcct gtacaagagt gcctacctgc acccccagca 4021 gtttgactgt gagcctgggg tgctaggcag caagggcttc acctggggca aggtctactg 4081 ggaagtggaa gtggagaggg agggctggtc tgaggatgaa gaagaggggg atgaggagga 4141 agagggagaa gaggaggagg aggaagagga ggccggctat ggggatggat atgacgactg 4201 ggaaacggac gaagatgagg aatcgttggg cgatgaagag gaagaagagg aggaggaaga 4261 ggaggaagtt ctggaaagct gcatggtggg ggtggctaga gactctgtga agaggaaggg 4321 agacctctcc ctgcggccag aggatggcgt gtgggcgctg cgcctctcct cctccggcat 4381 ctgggccaac accagccccg aggctgagct tttcccagca ctgcggcccc ggagagtggg 4441 catcgccctg gattatgaag ggggcaccgt gactttcacc aacgcagagt cacaggaact 4501 catctacacc ttcactgcca ccttcacccg gcgcctggtc cccttcctgt ggctcaagtg 4561 gccaggaaca cgcctcctgc taagacccgg atccgactac aaagaccatg acggtgatta 4621 taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg 4681 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 4741 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga 4801 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 4861 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa 4921 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc 4981 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc 5041 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag 5101 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt 5161 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct 5221 agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg 5281 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg 5341 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 5401 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 5461 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 5521 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 5581 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 5641 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 5701 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 5761 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 5821 gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5881 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5941 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 6001 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 6061 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 6121 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 6181 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 6241 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 6301 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6361 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 6421 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 6481 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 6541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 6721 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 6781 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6901 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 6961 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 7021 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 7081 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 7141 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 7201 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7321 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7381 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7441 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7501 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7561 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7681 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7861 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7921 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7981 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 8041 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 8101 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 8161 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8221 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8281 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8341 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8401 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8461 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8521 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8581 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8641 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8701 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8761 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8821 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8881 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8941 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 9001 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 9061 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 9121 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9241 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 9301 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 9361 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 9421 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 9481 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 9541 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 9601 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 9661 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 9721 ccagtgccaa gctg //