LOCUS       V027954                 9702 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027954
VERSION     V027954
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..3721
                     /label="TRIM21(NM_003141)"
                     /note="TRIM21(NM_003141)"
                     /gene="TRIM21"
     CDS             3728..3817
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     promoter        3861..4072
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4085..4353
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4382..4391
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4412..5074
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5144..5197
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5198..5797
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5798..6385
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6459..6692
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6764..6885
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6904..7039
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7077..7093)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7101..7117
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7125..7155)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7170..7191
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7479..8067)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8238..9098)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9099..9203)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9677..9693
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg cttcagcagc acgcttgaca atgatgtggg aggaggtcac
     2341 atgccctatc tgcctggacc ccttcgtgga gcctgtgagc atcgagtgtg gccacagctt
     2401 ctgccaggaa tgcatctctc aggttgggaa aggtgggggc agcgtctgtc ctgtgtgccg
     2461 gcagcgcttt ctgctcaaga atctccggcc caatcgacag ctagccaaca tggtgaacaa
     2521 ccttaaagaa atcagccagg aggccagaga gggcacacag ggggaacggt gtgcagtgca
     2581 tggagagaga cttcacctgt tctgtgagaa agatgggaag gccctttgct gggtatgtgc
     2641 ccagtctcgg aaacaccgtg accacgccat ggtccctctt gaggaggctg cacaggagta
     2701 ccaggagaag ctccaggtgg cattagggga actgagaaga aagcaggagt tggctgagaa
     2761 gttggaagtg gaaattgcaa taaagagagc agactggaag aaaacagtgg aaacacagaa
     2821 atctaggatt cacgcagagt ttgtgcagca aaaaaacttc ctggttgaag aagaacagag
     2881 gcagctgcag gagctggaga aggatgagag ggagcagctg agaatcctgg gggagaaaga
     2941 ggccaagctg gcccagcaga gccaggccct acaggagctc atctcagagc tagatcgaag
     3001 gtgccacagc tcagcactgg aactgctgca ggaggtgata attgtcctgg aaaggagtga
     3061 gtcctggaac ctgaaggacc tggatattac ctctccagaa ctcaggagtg tgtgccatgt
     3121 gccagggctg aagaagatgc tgaggacatg tgcagtccac atcactctgg atccagacac
     3181 agccaatccg tggctgatac tttcagaaga tcggagacaa gtgaggcttg gagacaccca
     3241 gcagagcata cctggaaatg aagagagatt tgatagttat cctatggtcc tgggtgccca
     3301 gcactttcac tctggaaaac attactggga ggtagatgtg acaggaaagg aggcctggga
     3361 cctgggtgtc tgcagagact ctgtgcgcag gaaggggcac tttttgctta gttccaagag
     3421 tggcttctgg acaatttggt tgtggaacaa acaaaaatat gaggctggca cctaccccca
     3481 gactcccctc caccttcagg tgcctccatg ccaagttggg attttcctgg actatgaggc
     3541 tggcatggtc tccttctaca acatcactga ccatggctcc ctcatctact ccttctctga
     3601 atgtgccttt acaggacctc tgcggccctt cttcagtcct ggtttcaatg atggaggaaa
     3661 aaacacagcc cctctaaccc tctgtccact gaatattgga tcacaaggat ccactgacta
     3721 tggatccgaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga
     3781 agatttagag caaaagttaa tttccgagga ggacttatag cggccgcgaa ggatctgcga
     3841 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     3901 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     3961 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4021 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     4081 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     4141 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     4201 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     4261 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     4321 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     4381 cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     4441 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     4501 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     4561 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     4621 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     4681 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     4741 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     4801 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     4861 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     4921 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     4981 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     5041 ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc
     5101 tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct
     5161 tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt
     5221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     5281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     5341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5401 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5761 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat
     5821 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5881 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5941 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6001 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6061 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6121 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6181 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     6241 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6301 cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg
     6361 gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     6421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6481 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6661 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6721 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6901 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6961 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7021 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     7081 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7141 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7261 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8521 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8821 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9301 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9361 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9421 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     9481 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
     9541 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     9601 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9661 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
