LOCUS       V043359                14823 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043359
VERSION     V043359
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..8905
                     /label="TNC(NM_002160)"
                     /note="TNC(NM_002160)"
                     /gene="TNC"
     CDS             8912..8977
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9021..9232
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9245..9513
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             9548..10255
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             10265..10318
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             10319..10918
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10919..11506
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11580..11813
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11885..12006
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12025..12160
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12198..12214)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12222..12238
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12246..12276)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12291..12312
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12600..13188)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13359..14219)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14220..14324)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14798..14814
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgggggc catgactcag ctgttggcag gtgtctttct
     2341 tgctttcctt gccctcgcta ccgaaggtgg ggtcctcaag aaagtcatcc ggcacaagcg
     2401 acagagtggg gtgaacgcca ccctgccaga agagaaccag ccagtggtgt ttaaccacgt
     2461 ttacaacatc aagctgccag tgggatccca gtgttcggtg gatctggagt cagccagtgg
     2521 ggagaaagac ctggcaccgc cttcagagcc cagcgaaagc tttcaggagc acacagtgga
     2581 tggggaaaac cagattgtct tcacacatcg catcaacatc ccccgccggg cctgtggctg
     2641 tgccgcagcc cctgatgtta aggagctgct gagcagactg gaggagctgg agaacctggt
     2701 gtcttccctg agggagcaat gtactgcagg agcaggctgc tgtctccagc ctgccacagg
     2761 ccgcttggac accaggccct tctgtagcgg tcggggcaac ttcagcactg aaggatgtgg
     2821 ctgtgtctgc gaacctggct ggaaaggccc caactgctct gagcccgaat gtccaggcaa
     2881 ctgtcacctt cgaggccggt gcattgatgg gcagtgcatc tgtgacgacg gcttcacggg
     2941 cgaggactgc agccagctgg cttgccccag cgactgcaat gaccagggca agtgcgtaaa
     3001 tggagtctgc atctgtttcg aaggctacgc cggggctgac tgcagccgtg aaatctgccc
     3061 agtgccctgc agtgaggagc acggcacatg tgtagatggc ttgtgtgtgt gccacgatgg
     3121 ctttgcaggc gatgactgca acaagcctct gtgtctcaac aattgctaca accgtggacg
     3181 atgcgtggag aatgagtgcg tgtgtgatga gggtttcacg ggcgaagact gcagtgagct
     3241 catctgcccc aatgactgct tcgaccgggg ccgctgcatc aatggcacct gctactgcga
     3301 agaaggcttc acaggtgaag actgcgggaa acccacctgc ccacatgcct gccacaccca
     3361 gggccggtgt gaggaggggc agtgtgtatg tgatgagggc tttgccggtg tggactgcag
     3421 cgagaagagg tgtcctgctg actgtcacaa tcgtggccgc tgtgtagacg ggcggtgtga
     3481 gtgtgatgat ggtttcactg gagctgactg tggggagctc aagtgtccca atggctgcag
     3541 tggccatggc cgctgtgtca atgggcagtg tgtgtgtgat gagggctata ctggggagga
     3601 ctgcagccag ctacggtgcc ccaatgactg tcacagtcgg ggccgctgtg tcgagggcaa
     3661 atgtgtatgt gagcaaggct tcaagggcta tgactgcagt gacatgagct gccctaatga
     3721 ctgtcaccag cacggccgct gtgtgaatgg catgtgtgtt tgtgatgacg gctacacagg
     3781 ggaagactgc cgggatcgcc aatgccccag ggactgcagc aacaggggcc tctgtgtgga
     3841 cggacagtgc gtctgtgagg acggcttcac cggccctgac tgtgcagaac tctcctgtcc
     3901 aaatgactgc catggccagg gtcgctgtgt gaatgggcag tgcgtgtgcc atgaaggatt
     3961 tatgggcaaa gactgcaagg agcaaagatg tcccagtgac tgtcatggcc agggccgctg
     4021 cgtggacggc cagtgcatct gccacgaggg cttcacaggc ctggactgtg gccagcactc
     4081 ctgccccagt gactgcaaca acttaggaca atgcgtctcg ggccgctgca tctgcaacga
     4141 gggctacagc ggagaagact gctcagaggt gtctcctccc aaagacctcg ttgtgacaga
     4201 agtgacggaa gagacggtca acctggcctg ggacaatgag atgcgggtca cagagtacct
     4261 tgtcgtgtac acgcccaccc acgagggtgg tctggaaatg cagttccgtg tgcctgggga
     4321 ccagacgtcc accatcatcc aggagctgga gcctggtgtg gagtacttta tccgtgtatt
     4381 tgccatcctg gagaacaaga agagcattcc tgtcagcgcc agggtggcca cgtacttacc
     4441 tgcacctgaa ggcctgaaat tcaagtccat caaggagaca tctgtggaag tggagtggga
     4501 tcctctagac attgcttttg aaacctggga gatcatcttc cggaatatga ataaagaaga
     4561 tgagggagag atcaccaaaa gcctgaggag gccagagacc tcttaccggc aaactggtct
     4621 agctcctggg caagagtatg agatatctct gcacatagtg aaaaacaata cccggggccc
     4681 tggcctgaag agggtgacca ccacacgctt ggatgccccc agccagatcg aggtgaaaga
     4741 tgtcacagac accactgcct tgatcacctg gttcaagccc ctggctgaga tcgatggcat
     4801 tgagctgacc tacggcatca aagacgtgcc aggagaccgt accaccatcg atctcacaga
     4861 ggacgagaac cagtactcca tcgggaacct gaagcctgac actgagtacg aggtgtccct
     4921 catctcccgc agaggtgaca tgtcaagcaa cccagccaaa gagaccttca caacaggcct
     4981 cgatgctccc aggaatcttc gacgtgtttc ccagacagat aacagcatca ccctggaatg
     5041 gaggaatggc aaggcagcta ttgacagtta cagaattaag tatgccccca tctctggagg
     5101 ggaccacgct gaggttgatg ttccaaagag ccaacaagcc acaaccaaaa ccacactcac
     5161 aggtctgagg ccgggaactg aatatgggat tggagtttct gctgtgaagg aagacaagga
     5221 gagcaatcca gcgaccatca acgcagccac agagttggac acgcccaagg accttcaggt
     5281 ttctgaaact gcagagacca gcctgaccct gctctggaag acaccgttgg ccaaatttga
     5341 ccgctaccgc ctcaattaca gtctccccac aggccagtgg gtgggagtgc agcttccaag
     5401 aaacaccact tcctatgtcc tgagaggcct ggaaccagga caggagtaca atgtcctcct
     5461 gacagccgag aaaggcagac acaagagcaa gcccgcacgt gtgaaggcat ccactgaaca
     5521 agcccctgag ctggaaaacc tcaccgtgac tgaggttggc tgggatggcc tcagactcaa
     5581 ctggaccgca gctgaccagg cctatgagca ctttatcatt caggtgcagg aggccaacaa
     5641 ggtggaggca gctcggaacc tcaccgtgcc tggcagcctt cgggctgtgg acataccggg
     5701 cctcaaggct gctacgcctt atacagtctc catctatggg gtgatccagg gctatagaac
     5761 accagtgctc tctgctgagg cctccacagg ggaaactccc aatttgggag aggtcgtggt
     5821 ggccgaggtg ggctgggatg ccctcaaact caactggact gctccagaag gggcctatga
     5881 gtactttttc attcaggtgc aggaggctga cacagtagag gcagcccaga acctcaccgt
     5941 cccaggagga ctgaggtcca cagacctgcc tgggctcaaa gcagccactc attataccat
     6001 caccatccgc ggggtcactc aggacttcag cacaacccct ctctctgttg aagtcttgac
     6061 agaggaggtt ccagatatgg gaaacctcac agtgaccgag gttagctggg atgctctcag
     6121 actgaactgg accacgccag atggaaccta tgaccagttt actattcagg tccaggaggc
     6181 tgaccaggtg gaagaggctc acaatctcac ggttcctggc agcctgcgtt ccatggaaat
     6241 cccaggcctc agggctggca ctccttacac agtcaccctg cacggcgagg tcaggggcca
     6301 cagcactcga ccccttgctg tagaggtcgt cacagaggat ctcccacagc tgggagattt
     6361 agccgtgtct gaggttggct gggatggcct cagactcaac tggaccgcag ctgacaatgc
     6421 ctatgagcac tttgtcattc aggtgcagga ggtcaacaaa gtggaggcag cccagaacct
     6481 cacgttgcct ggcagcctca gggctgtgga catcccgggc ctcgaggctg ccacgcctta
     6541 tagagtctcc atctatgggg tgatccgggg ctatagaaca ccagtactct ctgctgaggc
     6601 ctccacagcc aaagaacctg aaattggaaa cttaaatgtt tctgacataa ctcccgagag
     6661 cttcaatctc tcctggatgg ctaccgatgg gatcttcgag acctttacca ttgaaattat
     6721 tgattccaat aggttgctgg agactgtgga atataatatc tctggtgctg aacgaactgc
     6781 ccatatctca gggctacccc ctagtactga ttttattgtc tacctctctg gacttgctcc
     6841 cagcatccgg accaaaacca tcagtgccac agccacgaca gaggccctgc cccttctgga
     6901 aaacctaacc atttccgaca ttaatcccta cgggttcaca gtttcctgga tggcatcgga
     6961 gaatgccttt gacagctttc tagtaacggt ggtggattct gggaagctgc tggaccccca
     7021 ggaattcaca ctttcaggaa cccagaggaa gctggagctt agaggcctca taactggcat
     7081 tggctatgag gttatggtct ctggcttcac ccaagggcat caaaccaagc ccttgagggc
     7141 tgagattgtt acagaagccg aaccggaagt tgacaacctt ctggtttcag atgccacccc
     7201 agacggtttc cgtctgtcct ggacagctga tgaaggggtc ttcgacaatt ttgttctcaa
     7261 aatcagagat accaaaaagc agtctgagcc actggaaata accctacttg cccccgaacg
     7321 taccagggac ataacaggtc tcagagaggc tactgaatac gaaattgaac tctatggaat
     7381 aagcaaagga aggcgatccc agacagtcag tgctatagca acaacagcca tgggctcccc
     7441 aaaggaagtc attttctcag acatcactga aaattcggct actgtcagct ggagggcacc
     7501 cacagcccaa gtggagagct tccggattac ctatgtgccc attacaggag gtacaccctc
     7561 catggtaact gtggacggaa ccaagactca gaccaggctg gtgaaactca tacctggcgt
     7621 ggagtacctt gtcagcatca tcgccatgaa gggctttgag gaaagtgaac ctgtctcagg
     7681 gtcattcacc acagctctgg atggcccatc tggcctggtg acagccaaca tcactgactc
     7741 agaagccttg gccaggtggc agccagccat tgccactgtg gacagttatg tcatctccta
     7801 cacaggcgag aaagtgccag aaattacacg cacggtgtcc gggaacacag tggagtatgc
     7861 tctgaccgac ctcgagcctg ccacggaata cacactgaga atctttgcag agaaagggcc
     7921 ccagaagagc tcaaccatca ctgccaagtt cacaacagac ctcgattctc caagagactt
     7981 gactgctact gaggttcagt cggaaactgc cctccttacc tggcgacccc cccgggcatc
     8041 agtcaccggt tacctgctgg tctatgaatc agtggatggc acagtcaagg aagtcattgt
     8101 gggtccagat accacctcct acagcctggc agacctgagc ccatccaccc actacacagc
     8161 caagatccag gcactcaatg ggcccctgag gagcaatatg atccagacca tcttcaccac
     8221 aattggactc ctgtacccct tccccaagga ctgctcccaa gcaatgctga atggagacac
     8281 gacctctggc ctctacacca tttatctgaa tggtgataag gctgaggcgc tggaagtctt
     8341 ctgtgacatg acctctgatg ggggtggatg gattgtgttc ctgagacgca aaaacggacg
     8401 cgagaacttc taccaaaact ggaaggcata tgctgctgga tttggggacc gcagagaaga
     8461 attctggctt gggctggaca acctgaacaa aatcacagcc caggggcagt acgagctccg
     8521 ggtggacctg cgggaccatg gggagacagc ctttgctgtc tatgacaagt tcagcgtggg
     8581 agatgccaag actcgctaca agctgaaggt ggaggggtac agtgggacag caggtgactc
     8641 catggcctac cacaatggca gatccttctc cacctttgac aaggacacag attcagccat
     8701 caccaactgt gctctgtcct acaaaggggc tttctggtac aggaactgtc accgtgtcaa
     8761 cctgatgggg agatatgggg acaataacca cagtcagggc gttaactggt tccactggaa
     8821 gggccacgaa cactcaatcc agtttgctga gatgaagctg agaccaagca acttcagaaa
     8881 tcttgaaggc aggcgcaaac gggcaggatc cgactacaaa gaccatgacg gtgattataa
     8941 agatcatgac atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga
     9001 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     9061 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     9121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     9181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     9241 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     9301 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     9361 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     9421 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     9481 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     9541 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     9601 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     9661 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     9721 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     9781 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     9841 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     9901 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     9961 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
    10021 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
    10081 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
    10141 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
    10201 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggatc
    10261 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat
    10321 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10381 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10441 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10501 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    10561 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10621 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    10681 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    10741 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10801 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    10861 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa
    10921 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    10981 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    11041 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    11101 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    11161 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    11221 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    11281 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
    11341 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    11401 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg
    11461 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag
    11521 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
    11581 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct
    11641 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    11701 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    11761 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
    11821 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
    11881 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    11941 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12001 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
    12061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    12121 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
    12181 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    12241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    12301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    12361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    12421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    12481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    12541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    12601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    12661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    12721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    12781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    12841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    12901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    12961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    13021 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    13081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    13141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    13201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    13261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    13321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    13381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    13441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    13501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    13561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    13621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    13681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    13741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    13801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    13861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    13921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    13981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    14041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    14101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    14161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    14221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    14281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    14341 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    14401 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
    14461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
    14521 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    14581 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    14641 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    14701 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    14761 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    14821 ctg
//
