LOCUS V043359 14823 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043359 VERSION V043359 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..8905 /label="TNC(NM_002160)" /note="TNC(NM_002160)" /gene="TNC" CDS 8912..8977 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 9021..9232 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 9245..9513 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 9548..10255 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 10265..10318 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 10319..10918 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 10919..11506 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 11580..11813 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 11885..12006 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 12025..12160 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(12198..12214) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 12222..12238 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(12246..12276) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 12291..12312 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(12600..13188) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(13359..14219) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(14220..14324) /gene="bla" /label="AmpR promoter" primer_bind 14798..14814 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgggggc catgactcag ctgttggcag gtgtctttct 2341 tgctttcctt gccctcgcta ccgaaggtgg ggtcctcaag aaagtcatcc ggcacaagcg 2401 acagagtggg gtgaacgcca ccctgccaga agagaaccag ccagtggtgt ttaaccacgt 2461 ttacaacatc aagctgccag tgggatccca gtgttcggtg gatctggagt cagccagtgg 2521 ggagaaagac ctggcaccgc cttcagagcc cagcgaaagc tttcaggagc acacagtgga 2581 tggggaaaac cagattgtct tcacacatcg catcaacatc ccccgccggg cctgtggctg 2641 tgccgcagcc cctgatgtta aggagctgct gagcagactg gaggagctgg agaacctggt 2701 gtcttccctg agggagcaat gtactgcagg agcaggctgc tgtctccagc ctgccacagg 2761 ccgcttggac accaggccct tctgtagcgg tcggggcaac ttcagcactg aaggatgtgg 2821 ctgtgtctgc gaacctggct ggaaaggccc caactgctct gagcccgaat gtccaggcaa 2881 ctgtcacctt cgaggccggt gcattgatgg gcagtgcatc tgtgacgacg gcttcacggg 2941 cgaggactgc agccagctgg cttgccccag cgactgcaat gaccagggca agtgcgtaaa 3001 tggagtctgc atctgtttcg aaggctacgc cggggctgac tgcagccgtg aaatctgccc 3061 agtgccctgc agtgaggagc acggcacatg tgtagatggc ttgtgtgtgt gccacgatgg 3121 ctttgcaggc gatgactgca acaagcctct gtgtctcaac aattgctaca accgtggacg 3181 atgcgtggag aatgagtgcg tgtgtgatga gggtttcacg ggcgaagact gcagtgagct 3241 catctgcccc aatgactgct tcgaccgggg ccgctgcatc aatggcacct gctactgcga 3301 agaaggcttc acaggtgaag actgcgggaa acccacctgc ccacatgcct gccacaccca 3361 gggccggtgt gaggaggggc agtgtgtatg tgatgagggc tttgccggtg tggactgcag 3421 cgagaagagg tgtcctgctg actgtcacaa tcgtggccgc tgtgtagacg ggcggtgtga 3481 gtgtgatgat ggtttcactg gagctgactg tggggagctc aagtgtccca atggctgcag 3541 tggccatggc cgctgtgtca atgggcagtg tgtgtgtgat gagggctata ctggggagga 3601 ctgcagccag ctacggtgcc ccaatgactg tcacagtcgg ggccgctgtg tcgagggcaa 3661 atgtgtatgt gagcaaggct tcaagggcta tgactgcagt gacatgagct gccctaatga 3721 ctgtcaccag cacggccgct gtgtgaatgg catgtgtgtt tgtgatgacg gctacacagg 3781 ggaagactgc cgggatcgcc aatgccccag ggactgcagc aacaggggcc tctgtgtgga 3841 cggacagtgc gtctgtgagg acggcttcac cggccctgac tgtgcagaac tctcctgtcc 3901 aaatgactgc catggccagg gtcgctgtgt gaatgggcag tgcgtgtgcc atgaaggatt 3961 tatgggcaaa gactgcaagg agcaaagatg tcccagtgac tgtcatggcc agggccgctg 4021 cgtggacggc cagtgcatct gccacgaggg cttcacaggc ctggactgtg gccagcactc 4081 ctgccccagt gactgcaaca acttaggaca atgcgtctcg ggccgctgca tctgcaacga 4141 gggctacagc ggagaagact gctcagaggt gtctcctccc aaagacctcg ttgtgacaga 4201 agtgacggaa gagacggtca acctggcctg ggacaatgag atgcgggtca cagagtacct 4261 tgtcgtgtac acgcccaccc acgagggtgg tctggaaatg cagttccgtg tgcctgggga 4321 ccagacgtcc accatcatcc aggagctgga gcctggtgtg gagtacttta tccgtgtatt 4381 tgccatcctg gagaacaaga agagcattcc tgtcagcgcc agggtggcca cgtacttacc 4441 tgcacctgaa ggcctgaaat tcaagtccat caaggagaca tctgtggaag tggagtggga 4501 tcctctagac attgcttttg aaacctggga gatcatcttc cggaatatga ataaagaaga 4561 tgagggagag atcaccaaaa gcctgaggag gccagagacc tcttaccggc aaactggtct 4621 agctcctggg caagagtatg agatatctct gcacatagtg aaaaacaata cccggggccc 4681 tggcctgaag agggtgacca ccacacgctt ggatgccccc agccagatcg aggtgaaaga 4741 tgtcacagac accactgcct tgatcacctg gttcaagccc ctggctgaga tcgatggcat 4801 tgagctgacc tacggcatca aagacgtgcc aggagaccgt accaccatcg atctcacaga 4861 ggacgagaac cagtactcca tcgggaacct gaagcctgac actgagtacg aggtgtccct 4921 catctcccgc agaggtgaca tgtcaagcaa cccagccaaa gagaccttca caacaggcct 4981 cgatgctccc aggaatcttc gacgtgtttc ccagacagat aacagcatca ccctggaatg 5041 gaggaatggc aaggcagcta ttgacagtta cagaattaag tatgccccca tctctggagg 5101 ggaccacgct gaggttgatg ttccaaagag ccaacaagcc acaaccaaaa ccacactcac 5161 aggtctgagg ccgggaactg aatatgggat tggagtttct gctgtgaagg aagacaagga 5221 gagcaatcca gcgaccatca acgcagccac agagttggac acgcccaagg accttcaggt 5281 ttctgaaact gcagagacca gcctgaccct gctctggaag acaccgttgg ccaaatttga 5341 ccgctaccgc ctcaattaca gtctccccac aggccagtgg gtgggagtgc agcttccaag 5401 aaacaccact tcctatgtcc tgagaggcct ggaaccagga caggagtaca atgtcctcct 5461 gacagccgag aaaggcagac acaagagcaa gcccgcacgt gtgaaggcat ccactgaaca 5521 agcccctgag ctggaaaacc tcaccgtgac tgaggttggc tgggatggcc tcagactcaa 5581 ctggaccgca gctgaccagg cctatgagca ctttatcatt caggtgcagg aggccaacaa 5641 ggtggaggca gctcggaacc tcaccgtgcc tggcagcctt cgggctgtgg acataccggg 5701 cctcaaggct gctacgcctt atacagtctc catctatggg gtgatccagg gctatagaac 5761 accagtgctc tctgctgagg cctccacagg ggaaactccc aatttgggag aggtcgtggt 5821 ggccgaggtg ggctgggatg ccctcaaact caactggact gctccagaag gggcctatga 5881 gtactttttc attcaggtgc aggaggctga cacagtagag gcagcccaga acctcaccgt 5941 cccaggagga ctgaggtcca cagacctgcc tgggctcaaa gcagccactc attataccat 6001 caccatccgc ggggtcactc aggacttcag cacaacccct ctctctgttg aagtcttgac 6061 agaggaggtt ccagatatgg gaaacctcac agtgaccgag gttagctggg atgctctcag 6121 actgaactgg accacgccag atggaaccta tgaccagttt actattcagg tccaggaggc 6181 tgaccaggtg gaagaggctc acaatctcac ggttcctggc agcctgcgtt ccatggaaat 6241 cccaggcctc agggctggca ctccttacac agtcaccctg cacggcgagg tcaggggcca 6301 cagcactcga ccccttgctg tagaggtcgt cacagaggat ctcccacagc tgggagattt 6361 agccgtgtct gaggttggct gggatggcct cagactcaac tggaccgcag ctgacaatgc 6421 ctatgagcac tttgtcattc aggtgcagga ggtcaacaaa gtggaggcag cccagaacct 6481 cacgttgcct ggcagcctca gggctgtgga catcccgggc ctcgaggctg ccacgcctta 6541 tagagtctcc atctatgggg tgatccgggg ctatagaaca ccagtactct ctgctgaggc 6601 ctccacagcc aaagaacctg aaattggaaa cttaaatgtt tctgacataa ctcccgagag 6661 cttcaatctc tcctggatgg ctaccgatgg gatcttcgag acctttacca ttgaaattat 6721 tgattccaat aggttgctgg agactgtgga atataatatc tctggtgctg aacgaactgc 6781 ccatatctca gggctacccc ctagtactga ttttattgtc tacctctctg gacttgctcc 6841 cagcatccgg accaaaacca tcagtgccac agccacgaca gaggccctgc cccttctgga 6901 aaacctaacc atttccgaca ttaatcccta cgggttcaca gtttcctgga tggcatcgga 6961 gaatgccttt gacagctttc tagtaacggt ggtggattct gggaagctgc tggaccccca 7021 ggaattcaca ctttcaggaa cccagaggaa gctggagctt agaggcctca taactggcat 7081 tggctatgag gttatggtct ctggcttcac ccaagggcat caaaccaagc ccttgagggc 7141 tgagattgtt acagaagccg aaccggaagt tgacaacctt ctggtttcag atgccacccc 7201 agacggtttc cgtctgtcct ggacagctga tgaaggggtc ttcgacaatt ttgttctcaa 7261 aatcagagat accaaaaagc agtctgagcc actggaaata accctacttg cccccgaacg 7321 taccagggac ataacaggtc tcagagaggc tactgaatac gaaattgaac tctatggaat 7381 aagcaaagga aggcgatccc agacagtcag tgctatagca acaacagcca tgggctcccc 7441 aaaggaagtc attttctcag acatcactga aaattcggct actgtcagct ggagggcacc 7501 cacagcccaa gtggagagct tccggattac ctatgtgccc attacaggag gtacaccctc 7561 catggtaact gtggacggaa ccaagactca gaccaggctg gtgaaactca tacctggcgt 7621 ggagtacctt gtcagcatca tcgccatgaa gggctttgag gaaagtgaac ctgtctcagg 7681 gtcattcacc acagctctgg atggcccatc tggcctggtg acagccaaca tcactgactc 7741 agaagccttg gccaggtggc agccagccat tgccactgtg gacagttatg tcatctccta 7801 cacaggcgag aaagtgccag aaattacacg cacggtgtcc gggaacacag tggagtatgc 7861 tctgaccgac ctcgagcctg ccacggaata cacactgaga atctttgcag agaaagggcc 7921 ccagaagagc tcaaccatca ctgccaagtt cacaacagac ctcgattctc caagagactt 7981 gactgctact gaggttcagt cggaaactgc cctccttacc tggcgacccc cccgggcatc 8041 agtcaccggt tacctgctgg tctatgaatc agtggatggc acagtcaagg aagtcattgt 8101 gggtccagat accacctcct acagcctggc agacctgagc ccatccaccc actacacagc 8161 caagatccag gcactcaatg ggcccctgag gagcaatatg atccagacca tcttcaccac 8221 aattggactc ctgtacccct tccccaagga ctgctcccaa gcaatgctga atggagacac 8281 gacctctggc ctctacacca tttatctgaa tggtgataag gctgaggcgc tggaagtctt 8341 ctgtgacatg acctctgatg ggggtggatg gattgtgttc ctgagacgca aaaacggacg 8401 cgagaacttc taccaaaact ggaaggcata tgctgctgga tttggggacc gcagagaaga 8461 attctggctt gggctggaca acctgaacaa aatcacagcc caggggcagt acgagctccg 8521 ggtggacctg cgggaccatg gggagacagc ctttgctgtc tatgacaagt tcagcgtggg 8581 agatgccaag actcgctaca agctgaaggt ggaggggtac agtgggacag caggtgactc 8641 catggcctac cacaatggca gatccttctc cacctttgac aaggacacag attcagccat 8701 caccaactgt gctctgtcct acaaaggggc tttctggtac aggaactgtc accgtgtcaa 8761 cctgatgggg agatatgggg acaataacca cagtcagggc gttaactggt tccactggaa 8821 gggccacgaa cactcaatcc agtttgctga gatgaagctg agaccaagca acttcagaaa 8881 tcttgaaggc aggcgcaaac gggcaggatc cgactacaaa gaccatgacg gtgattataa 8941 agatcatgac atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga 9001 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 9061 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag 9121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 9181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct 9241 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg 9301 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt 9361 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct 9421 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt 9481 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga 9541 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 9601 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 9661 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 9721 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 9781 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 9841 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct 9901 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 9961 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 10021 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 10081 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta 10141 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 10201 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggatc 10261 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat 10321 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 10381 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 10441 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 10501 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 10561 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 10621 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 10681 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 10741 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 10801 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 10861 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa 10921 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 10981 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 11041 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 11101 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 11161 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 11221 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 11281 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc 11341 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 11401 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg 11461 ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag 11521 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 11581 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct 11641 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 11701 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 11761 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 11821 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 11881 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 11941 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 12001 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct 12061 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 12121 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc 12181 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 12241 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 12301 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 12361 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 12421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 12481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 12541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 12601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 12661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 12721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 12781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 12841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 12901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 12961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 13021 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 13081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 13141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 13201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 13261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 13321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 13381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 13441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 13501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 13561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 13621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 13681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 13741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 13801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 13861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 13921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 13981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 14041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 14101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 14161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 14221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 14281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 14341 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 14401 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 14461 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 14521 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag 14581 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 14641 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg 14701 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 14761 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 14821 ctg //