LOCUS       V042231                14347 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042231
VERSION     V042231
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..8373
                     /label="TNC(NM_001410991)"
                     /note="TNC(NM_001410991)"
                     /gene="TNC"
     CDS             8380..8445
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        8489..8700
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             8713..8981
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      9010..9019
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             9040..9702
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             9772..9825
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             9826..10425
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    10426..11014
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11088..11321
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    11393..11514
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      11554..11689
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(11722..11738)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    11746..11762
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(11770..11800)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    11815..11836
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12124..12712)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12883..13743)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(13744..13848)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     14322..14338
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gggccatgac tcagctgttg
     2341 gcaggtgtct ttcttgcttt ccttgccctc gctaccgaag gtggggtcct caagaaagtc
     2401 atccggcaca agcgacagag tggggtgaac gccaccctgc cagaagagaa ccagccagtg
     2461 gtgtttaacc acgtttacaa catcaagctg ccagtgggat cccagtgttc ggtggatctg
     2521 gagtcagcca gtggggagaa agacctggca ccgccttcag agcccagcga aagctttcag
     2581 gagcacacag tggatgggga aaaccagatt gtcttcacac atcgcatcaa catcccccgc
     2641 cgggcctgtg gctgtgccgc agcccctgat gttaaggagc tgctgagcag actggaggag
     2701 ctggagaacc tggtgtcttc cctgagggag caatgtactg caggagcagg ctgctgtctc
     2761 cagcctgcca caggccgctt ggacaccagg cccttctgta gcggtcgggg caacttcagc
     2821 actgaaggat gtggctgtgt ctgcgaacct ggctggaaag gccccaactg ctctgagccc
     2881 gaatgtccag gcaactgtca ccttcgaggc cggtgcattg atgggcagtg catctgtgac
     2941 gacggcttca cgggcgagga ctgcagccag ctggcttgcc ccagcgactg caatgaccag
     3001 ggcaagtgcg taaatggagt ctgcatctgt ttcgaaggct acgccggggc tgactgcagc
     3061 cgtgaaatct gcccagtgcc ctgcagtgag gagcacggca catgtgtaga tggcttgtgt
     3121 gtgtgccacg atggctttgc aggcgatgac tgcaacaagc ctctgtgtct caacaattgc
     3181 tacaaccgtg gacgatgcgt ggagaatgag tgcgtgtgtg atgagggttt cacgggcgaa
     3241 gactgcagtg agctcatctg ccccaatgac tgcttcgacc ggggccgctg catcaatggc
     3301 acctgctact gcgaagaagg cttcacaggt gaagactgcg ggaaacccac ctgcccacat
     3361 gcctgccaca cccagggccg gtgtgaggag gggcagtgtg tatgtgatga gggctttgcc
     3421 ggtgtggact gcagcgagaa gaggtgtcct gctgactgtc acaatcgtgg ccgctgtgta
     3481 gacgggcggt gtgagtgtga tgatggtttc actggagctg actgtgggga gctcaagtgt
     3541 cccaatggct gcagtggcca tggccgctgt gtcaatgggc agtgtgtgtg tgatgagggc
     3601 tatactgggg aggactgcag ccagctacgg tgccccaatg actgtcacag tcggggccgc
     3661 tgtgtcgagg gcaaatgtgt atgtgagcaa ggcttcaagg gctatgactg cagtgacatg
     3721 agctgcccta atgactgtca ccagcacggc cgctgtgtga atggcatgtg tgtttgtgat
     3781 gacggctaca caggggaaga ctgccgggat cgccaatgcc ccagggactg cagcaacagg
     3841 ggcctctgtg tggacggaca gtgcgtctgt gaggacggct tcaccggccc tgactgtgca
     3901 gaactctcct gtccaaatga ctgccatggc cagggtcgct gtgtgaatgg gcagtgcgtg
     3961 tgccatgaag gatttatggg caaagactgc aaggagcaaa gatgtcccag tgactgtcat
     4021 ggccagggcc gctgcgtgga cggccagtgc atctgccacg agggcttcac aggcctggac
     4081 tgtggccagc actcctgccc cagtgactgc aacaacttag gacaatgcgt ctcgggccgc
     4141 tgcatctgca acgagggcta cagcggagaa gactgctcag aggtgtctcc tcccaaagac
     4201 ctcgttgtga cagaagtgac ggaagagacg gtcaacctgg cctgggacaa tgagatgcgg
     4261 gtcacagagt accttgtcgt gtacacgccc acccacgagg gtggtctgga aatgcagttc
     4321 cgtgtgcctg gggaccagac gtccaccatc atccaggagc tggagcctgg tgtggagtac
     4381 tttatccgtg tatttgccat cctggagaac aagaagagca ttcctgtcag cgccagggtg
     4441 gccacgtact tacctgcacc tgaaggcctg aaattcaagt ccatcaagga gacatctgtg
     4501 gaagtggagt gggatcctct agacattgct tttgaaacct gggagatcat cttccggaat
     4561 atgaataaag aagatgaggg agagatcacc aaaagcctga ggaggccaga gacctcttac
     4621 cggcaaactg gtctagctcc tgggcaagag tatgagatat ctctgcacat agtgaaaaac
     4681 aatacccggg gccctggcct gaagagggtg accaccacac gcttggatgc ccccagccag
     4741 atcgaggtga aagatgtcac agacaccact gccttgatca cctggttcaa gcccctggct
     4801 gagatcgatg gcattgagct gacctacggc atcaaagacg tgccaggaga ccgtaccacc
     4861 atcgatctca cagaggacga gaaccagtac tccatcggga acctgaagcc tgacactgag
     4921 tacgaggtgt ccctcatctc ccgcagaggt gacatgtcaa gcaacccagc caaagagacc
     4981 ttcacaacag gcctcgatgc tcccaggaat cttcgacgtg tttcccagac agataacagc
     5041 atcaccctgg aatggaggaa tggcaaggca gctattgaca gttacagaat taagtatgcc
     5101 cccatctctg gaggggacca cgctgaggtt gatgttccaa agagccaaca agccacaacc
     5161 aaaaccacac tcacaggtct gaggccggga actgaatatg ggattggagt ttctgctgtg
     5221 aaggaagaca aggagagcaa tccagcgacc atcaacgcag ccacagagtt ggacacgccc
     5281 aaggaccttc aggtttctga aactgcagag accagcctga ccctgctctg gaagacaccg
     5341 ttggccaaat ttgaccgcta ccgcctcaat tacagtctcc ccacaggcca gtgggtggga
     5401 gtgcagcttc caagaaacac cacttcctat gtcctgagag gcctggaacc aggacaggag
     5461 tacaatgtcc tcctgacagc cgagaaaggc agacacaaga gcaagcccgc acgtgtgaag
     5521 gcatccactg aacaagcccc tgagctggaa aacctcaccg tgactgaggt tggctgggat
     5581 ggcctcagac tcaactggac cgcagctgac caggcctatg agcactttat cattcaggtg
     5641 caggaggcca acaaggtgga ggcagctcgg aacctcaccg tgcctggcag ccttcgggct
     5701 gtggacatac cgggcctcaa ggctgctacg ccttatacag tctccatcta tggggtgatc
     5761 cagggctata gaacaccagt gctctctgct gaggcctcca caggggaaac tcccaatttg
     5821 ggagaggtcg tggtggccga ggtgggctgg gatgccctca aactcaactg gactgctcca
     5881 gaaggggcct atgagtactt tttcattcag gtgcaggagg ctgacacagt agaggcagcc
     5941 cagaacctca ccgtcccagg aggactgagg tccacagacc tgcctgggct caaagcagcc
     6001 actcattata ccatcaccat ccgcggggtc actcaggact tcagcacaac ccctctctct
     6061 gttgaagtct tgacagagga tctcccacag ctgggagatt tagccgtgtc tgaggttggc
     6121 tgggatggcc tcagactcaa ctggaccgca gctgacaatg cctatgagca ctttgtcatt
     6181 caggtgcagg aggtcaacaa agtggaggca gcccagaacc tcacgttgcc tggcagcctc
     6241 agggctgtgg acatcccggg cctcgaggct gccacgcctt atagagtctc catctatggg
     6301 gtgatccggg gctatagaac accagtactc tctgctgagg cctccacagc caaagaacct
     6361 gaaattggaa acttaaatgt ttctgacata actcccgaga gcttcaatct ctcctggatg
     6421 gctaccgatg ggatcttcga gacctttacc attgaaatta ttgattccaa taggttgctg
     6481 gagactgtgg aatataatat ctctggtgct gaacgaactg cccatatctc agggctaccc
     6541 cctagtactg attttattgt ctacctctct ggacttgctc ccagcatccg gaccaaaacc
     6601 atcagtgcca cagccacgac agaagccgaa ccggaagttg acaaccttct ggtttcagat
     6661 gccaccccag acggtttccg tctgtcctgg acagctgatg aaggggtctt cgacaatttt
     6721 gttctcaaaa tcagagatac caaaaagcag tctgagccac tggaaataac cctacttgcc
     6781 cccgaacgta ccagggacat aacaggtctc agagaggcta ctgaatacga aattgaactc
     6841 tatggaataa gcaaaggaag gcgatcccag acagtcagtg ctatagcaac aacagccatg
     6901 ggctccccaa aggaagtcat tttctcagac atcactgaaa attcggctac tgtcagctgg
     6961 agggcaccca cagcccaagt ggagagcttc cggattacct atgtgcccat tacaggaggt
     7021 acaccctcca tggtaactgt ggacggaacc aagactcaga ccaggctggt gaaactcata
     7081 cctggcgtgg agtaccttgt cagcatcatc gccatgaagg gctttgagga aagtgaacct
     7141 gtctcagggt cattcaccac agctctggat ggcccatctg gcctggtgac agccaacatc
     7201 actgactcag aagccttggc caggtggcag ccagccattg ccactgtgga cagttatgtc
     7261 atctcctaca caggcgagaa agtgccagaa attacacgca cggtgtccgg gaacacagtg
     7321 gagtatgctc tgaccgacct cgagcctgcc acggaataca cactgagaat ctttgcagag
     7381 aaagggcccc agaagagctc aaccatcact gccaagttca caacagacct cgattctcca
     7441 agagacttga ctgctactga ggttcagtcg gaaactgccc tccttacctg gcgacccccc
     7501 cgggcatcag tcaccggtta cctgctggtc tatgaatcag tggatggcac agtcaaggaa
     7561 gtcattgtgg gtccagatac cacctcctac agcctggcag acctgagccc atccacccac
     7621 tacacagcca agatccaggc actcaatggg cccctgagga gcaatatgat ccagaccatc
     7681 ttcaccacaa ttggactcct gtaccccttc cccaaggact gctcccaagc aatgctgaat
     7741 ggagacacga cctctggcct ctacaccatt tatctgaatg gtgataaggc tgaggcgctg
     7801 gaagtcttct gtgacatgac ctctgatggg ggtggatgga ttgtgttcct gagacgcaaa
     7861 aacggacgcg agaacttcta ccaaaactgg aaggcatatg ctgctggatt tggggaccgc
     7921 agagaagaat tctggcttgg gctggacaac ctgaacaaaa tcacagccca ggggcagtac
     7981 gagctccggg tggacctgcg ggaccatggg gagacagcct ttgctgtcta tgacaagttc
     8041 agcgtgggag atgccaagac tcgctacaag ctgaaggtgg aggggtacag tgggacagca
     8101 ggtgactcca tggcctacca caatggcaga tccttctcca cctttgacaa ggacacagat
     8161 tcagccatca ccaactgtgc tctgtcctac aaaggggctt tctggtacag gaactgtcac
     8221 cgtgtcaacc tgatggggag atatggggac aataaccaca gtcagggcgt taactggttc
     8281 cactggaagg gccacgaaca ctcaatccag tttgctgaga tgaagctgag accaagcaac
     8341 ttcagaaatc ttgaaggcag gcgcaaacgg gcaggatccg actacaaaga ccatgacggt
     8401 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     8461 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     8521 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     8581 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     8641 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     8701 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     8761 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     8821 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     8881 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     8941 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     9001 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     9061 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     9121 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     9181 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     9241 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     9301 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     9361 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     9421 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     9481 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     9541 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     9601 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     9661 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     9721 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     9781 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     9841 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     9901 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     9961 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
    10021 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
    10081 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
    10141 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
    10201 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
    10261 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
    10321 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
    10381 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
    10441 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    10501 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    10561 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    10621 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    10681 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    10741 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    10801 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    10861 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    10921 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    10981 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
    11041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    11101 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
    11161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    11221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    11281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
    11341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
    11401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    11461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    11521 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    11581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    11641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
    11701 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
    11761 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
    11821 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
    11881 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    11941 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    12001 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    12061 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    12121 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    12181 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    12241 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    12301 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    12361 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    12421 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    12481 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    12541 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    12601 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    12661 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    12721 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    12781 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    12841 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    12901 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    12961 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    13021 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    13081 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    13141 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    13201 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    13261 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    13321 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    13381 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    13441 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    13501 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    13561 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    13621 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    13681 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    13741 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    13801 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    13861 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    13921 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    13981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    14041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    14101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    14161 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    14221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    14281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    14341 caagctg
//
