LOCUS       V043153                10637 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043153
VERSION     V043153
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4818
                     /label="TLR4(NM_138554)"
                     /note="TLR4(NM_138554)"
                     /gene="TLR4"
     CDS             4855..5565
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     promoter        5607..5818
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5831..6099
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6126..6725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6732..7320
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7394..7627
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7699..7820
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7839..7974
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8012..8028)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8036..8052
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8060..8090)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8105..8126
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8414..9002)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9173..10033)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10034..10138)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10612..10628
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgatgtct gcctcgcgcc tggctgggac tctgatccca
     2341 gccatggcct tcctctcctg cgtgagacca gaaagctggg agccctgcgt ggaggtggtt
     2401 cctaatatta cttatcaatg catggagctg aatttctaca aaatccccga caacctcccc
     2461 ttctcaacca agaacctgga cctgagcttt aatcccctga ggcatttagg cagctatagc
     2521 ttcttcagtt tcccagaact gcaggtgctg gatttatcca ggtgtgaaat ccagacaatt
     2581 gaagatgggg catatcagag cctaagccac ctctctacct taatattgac aggaaacccc
     2641 atccagagtt tagccctggg agccttttct ggactatcaa gtttacagaa gctggtggct
     2701 gtggagacaa atctagcatc tctagagaac ttccccattg gacatctcaa aactttgaaa
     2761 gaacttaatg tggctcacaa tcttatccaa tctttcaaat tacctgagta tttttctaat
     2821 ctgaccaatc tagagcactt ggacctttcc agcaacaaga ttcaaagtat ttattgcaca
     2881 gacttgcggg ttctacatca aatgccccta ctcaatctct ctttagacct gtccctgaac
     2941 cctatgaact ttatccaacc aggtgcattt aaagaaatta ggcttcataa gctgacttta
     3001 agaaataatt ttgatagttt aaatgtaatg aaaacttgta ttcaaggtct ggctggttta
     3061 gaagtccatc gtttggttct gggagaattt agaaatgaag gaaacttgga aaagtttgac
     3121 aaatctgctc tagagggcct gtgcaatttg accattgaag aattccgatt agcatactta
     3181 gactactacc tcgatgatat tattgactta tttaattgtt tgacaaatgt ttcttcattt
     3241 tccctggtga gtgtgactat tgaaagggta aaagactttt cttataattt cggatggcaa
     3301 catttagaat tagttaactg taaatttgga cagtttccca cattgaaact caaatctctc
     3361 aaaaggctta ctttcacttc caacaaaggt gggaatgctt tttcagaagt tgatctacca
     3421 agccttgagt ttctagatct cagtagaaat ggcttgagtt tcaaaggttg ctgttctcaa
     3481 agtgattttg ggacaaccag cctaaagtat ttagatctga gcttcaatgg tgttattacc
     3541 atgagttcaa acttcttggg cttagaacaa ctagaacatc tggatttcca gcattccaat
     3601 ttgaaacaaa tgagtgagtt ttcagtattc ctatcactca gaaacctcat ttaccttgac
     3661 atttctcata ctcacaccag agttgctttc aatggcatct tcaatggctt gtccagtctc
     3721 gaagtcttga aaatggctgg caattctttc caggaaaact tccttccaga tatcttcaca
     3781 gagctgagaa acttgacctt cctggacctc tctcagtgtc aactggagca gttgtctcca
     3841 acagcattta actcactctc cagtcttcag gtactaaata tgagccacaa caacttcttt
     3901 tcattggata cgtttcctta taagtgtctg aactccctcc aggttcttga ttacagtctc
     3961 aatcacataa tgacttccaa aaaacaggaa ctacagcatt ttccaagtag tctagctttc
     4021 ttaaatctta ctcagaatga ctttgcttgt acttgtgaac accagagttt cctgcaatgg
     4081 atcaaggacc agaggcagct cttggtggaa gttgaacgaa tggaatgtgc aacaccttca
     4141 gataagcagg gcatgcctgt gctgagtttg aatatcacct gtcagatgaa taagaccatc
     4201 attggtgtgt cggtcctcag tgtgcttgta gtatctgttg tagcagttct ggtctataag
     4261 ttctattttc acctgatgct tcttgctggc tgcataaagt atggtagagg tgaaaacatc
     4321 tatgatgcct ttgttatcta ctcaagccag gatgaggact gggtaaggaa tgagctagta
     4381 aagaatttag aagaaggggt gcctccattt cagctctgcc ttcactacag agactttatt
     4441 cccggtgtgg ccattgctgc caacatcatc catgaaggtt tccataaaag ccgaaaggtg
     4501 attgttgtgg tgtcccagca cttcatccag agccgctggt gtatctttga atatgagatt
     4561 gctcagacct ggcagtttct gagcagtcgt gctggtatca tcttcattgt cctgcagaag
     4621 gtggagaaga ccctgctcag gcagcaggtg gagctgtacc gccttctcag caggaacact
     4681 tacctggagt gggaggacag tgtcctgggg cggcacatct tctggagacg actcagaaaa
     4741 gccctgctgg atggtaaatc atggaatcca gaaggaacag tgggtacagg atgcaattgg
     4801 caggaagcaa catctatcgg atccggtgga ggcggatcag gaggcggtgg atctatggtg
     4861 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     4921 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4981 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     5041 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     5101 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     5161 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     5221 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     5281 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     5341 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     5401 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     5461 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     5521 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaggcggc cgcgaaggat
     5581 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5641 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5701 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     5761 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     5821 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     5881 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     5941 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     6001 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     6061 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     6121 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6181 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6241 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6301 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6361 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6421 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6481 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6541 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6601 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6661 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6721 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6781 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6841 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6901 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6961 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7021 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7081 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7141 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7201 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7261 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7321 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7381 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7621 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7681 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7741 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7801 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     7981 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8821 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9301 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10141 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10201 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    10261 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10321 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10381 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10441 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10501 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10561 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10621 cggccagtgc caagctg
//
