LOCUS       V046140                12114 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046140
VERSION     V046140
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5199
                     /label="THBS4(NM_003248)"
                     /note="THBS4(NM_003248)"
                     /gene="THBS4"
     CDS             5206..5271
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             5272..6228
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID
                     engineered for improved catalytic efficiency (Branon et
                     al., 2018)"
                     /transl_table=1
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
                     /label="TurboID"
     promoter        6273..6484
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6497..6765
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6794..6803
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6824..7486
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7556..7609
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7610..8209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8210..8797
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8871..9104
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9176..9297
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9316..9451
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9489..9505)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9513..9529
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9537..9567)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9582..9603
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9891..10479)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10650..11510)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11511..11615)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12089..12105
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc tggccccgcg cggagccgcc
     2341 gtcctcctgc tgcacctggt cctgcagcgg tggctagcgg caggcgccca ggccaccccc
     2401 caggtctttg accttctccc atcttccagt cagaggctaa acccaggcgc tctgctgcca
     2461 gtcctgacag accccgccct gaatgatctc tatgtgattt ccaccttcaa gctgcagact
     2521 aaaagttcag ccaccatctt cggtctttac tcttcaactg acaacagtaa atattttgaa
     2581 tttactgtga tgggacgctt aaacaaagcc atcctccgtt acctgaagaa cgatgggaag
     2641 gtgcatttgg tggttttcaa caacctgcag ctggcagacg gaaggcggca caggatcctc
     2701 ctgaggctga gcaatttgca gcgaggggcc ggctccctag agctctacct ggactgcatc
     2761 caggtggatt ccgttcacaa tctccccagg gcctttgctg gcccctccca gaaacctgag
     2821 accattgaat tgaggacttt ccagaggaag ccacaggact tcttggaaga gctgaagctg
     2881 gtggtgagag gctcactgtt ccaggtggcc agcctgcaag actgcttcct gcagcagagt
     2941 gagccactgg ctgccacagg cacaggggac tttaaccggc agttcttggg tcaaatgaca
     3001 caattaaacc aactcctggg agaggtgaag gaccttctga gacagcaggt taaggaaaca
     3061 tcatttttgc gaaacaccat agctgaatgc caggcttgcg gtcctctcaa gtttcagtct
     3121 ccgaccccaa gcacggtggt gcccccggct ccccctgcac cgccaacacg cccacctcgt
     3181 cggtgtgact ccaacccatg tttccgaggt gtccaatgta ccgacagtag agatggcttc
     3241 cagtgtgggc cctgccccga gggctacaca ggaaacggga tcacctgtat tgatgttgat
     3301 gagtgcaaat accatccctg ctacccgggc gtgcactgca taaatttgtc tcctggcttc
     3361 agatgtgacg cctgcccagt gggcttcaca gggcccatgg tgcagggtgt tgggatcagt
     3421 tttgccaagt caaacaagca ggtctgcact gacattgatg agtgtcgaaa tggagcgtgc
     3481 gttcccaact cgatctgcgt taatactttg ggatcttacc gctgtgggcc ttgtaagccg
     3541 gggtatactg gtgatcagat aaggggatgc aaagcggaaa gaaactgcag aaacccagag
     3601 ctgaaccctt gcagtgtgaa tgcccagtgc attgaagaga ggcaggggga tgtgacatgt
     3661 gtgtgtggag tcggttgggc tggagatggc tatatctgtg gaaaggatgt ggacatcgac
     3721 agttaccccg acgaagaact gccatgctct gccaggaact gtaaaaagga caactgcaaa
     3781 tatgtgccaa attctggcca agaagatgca gacagagatg gcattggcga cgcttgtgac
     3841 gaggatgctg acggagatgg gatcctgaat gagcaggata actgtgtcct gattcataat
     3901 gtggaccaaa ggaacagcga taaagatatc tttggggatg cctgtgataa ctgcctgagt
     3961 gtcttaaata acgaccagaa agacaccgat ggggatggaa gaggagatgc ctgtgatgat
     4021 gacatggatg gagatggaat aaaaaacatt ctggacaact gcccaaaatt tcccaatcgt
     4081 gaccaacggg acaaggatgg tgatggtgtg ggggatgcct gtgacagttg tcctgatgtc
     4141 agcaacccta accagtctga tgtggataat gatctggttg gggactcctg tgacaccaat
     4201 caggacagtg atggagatgg gcaccaggac agcacagaca actgccccac cgtcattaac
     4261 agtgcccagc tggacaccga taaggatgga attggtgacg agtgtgatga tgatgatgac
     4321 aatgatggta tcccagacct ggtgccccct ggaccagaca actgccggct ggtccccaac
     4381 ccagcccagg aggatagcaa cagcgacgga gtgggagaca tctgtgagtc tgactttgac
     4441 caggaccagg tcatcgatcg gatcgacgtc tgcccagaga acgcagaggt caccctgacc
     4501 gacttcaggg cttaccagac cgtggtcctg gatcctgaag gggatgccca gatcgatccc
     4561 aactgggtgg tcctgaacca gggcatggag attgtacaga ccatgaacag tgatcctggc
     4621 ctggcagtgg ggtacacagc ttttaatgga gttgacttcg aagggacctt ccatgtgaat
     4681 acccagacag atgatgacta tgcaggcttt atctttggct accaagatag ctccagcttc
     4741 tacgtggtca tgtggaagca gacggagcag acatattggc aagccacccc attccgagca
     4801 gttgcagaac ctggcattca gctcaaggct gtgaagtcta agacaggtcc aggggagcat
     4861 ctccggaact ccctgtggca cacgggggac accagtgacc aggtcaggct gctgtggaag
     4921 gactccagga atgtgggctg gaaggacaag gtgtcctacc gctggttcct acagcacagg
     4981 ccccaggtgg gctacatcag ggtacgattt tatgaaggct ctgagttggt ggctgactct
     5041 ggcgtcacca tagacaccac aatgcgtgga ggccgacttg gcgttttctg cttctctcaa
     5101 gaaaacatca tctggtccaa cctcaagtat cgctgcaatg acaccatccc tgaggacttc
     5161 caagagtttc aaacccagaa tttcgaccgc ttcgataatg gatccgacta caaagaccat
     5221 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gaaagacaat
     5281 actgtgcctc tgaagctgat cgctctcctg gctaatggcg agttccatag tggcgaacag
     5341 ctgggagaaa ccctgggcat gtccagggcc gctatcaaca agcacattca gactctgcgc
     5401 gactggggcg tggacgtgtt caccgtgccc ggaaagggct actctctgcc cgagcctatc
     5461 ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg
     5521 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
     5581 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
     5641 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
     5701 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
     5761 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
     5821 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
     5881 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
     5941 ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctacgct gatccgagag
     6001 ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct gccacggtgg
     6061 gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt
     6121 ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt
     6181 atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaagta ggcggccgcg
     6241 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     6301 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     6361 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     6421 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     6481 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     6541 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     6601 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     6661 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     6721 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     6781 gcctacgcta gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga
     6841 gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg
     6901 cacccccaag cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt
     6961 cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc
     7021 cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg
     7081 catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc
     7141 cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat
     7201 cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga
     7261 taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag
     7321 cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa
     7381 cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg
     7441 catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca
     7501 gtcgtccaat tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg
     7561 cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta
     7621 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     7681 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7741 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7801 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7861 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7921 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7981 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8041 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8101 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8161 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct
     8221 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     8281 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8341 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8401 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8461 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8581 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     8641 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8701 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc
     8761 tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac
     8821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     8881 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga
     8941 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9001 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9061 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     9121 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     9181 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9241 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9301 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9421 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg
     9481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9541 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10321 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11641 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    11701 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    11761 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    11821 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    11881 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    11941 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    12001 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    12061 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
