LOCUS       V024971                10724 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024971
VERSION     V024971
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4767
                     /label="TGM1(NM_000359)"
                     /note="TGM1(NM_000359)"
                     /gene="TGM1"
     CDS             4774..4839
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4883..5094
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5107..5375
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5404..5413
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5434..6096
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6166..6219
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6220..6819
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6820..7407
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7481..7714
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7786..7907
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7926..8061
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8099..8115)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8123..8139
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8147..8177)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8192..8213
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8501..9089)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9260..10120)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10121..10225)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10699..10715
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga tggatgggcc acgttccgat
     2341 gtgggccgtt ggggtggcaa ccccttgcag ccccctacca cgccatctcc agagccagag
     2401 ccagagccag acggacgctc tcgcagagga ggaggccgtt ccttctgggc tcgctgctgt
     2461 ggctgctgtt catgccgaaa tgcggcagat gacgactggg gacctgaacc ctctgactcc
     2521 aggggtcgag ggtccagctc tggcactcga agacctggct cccggggctc agactcccgc
     2581 cggcctgtat cccggggcag cggtgtcaat gcagctggag atggcaccat ccgagagggc
     2641 atgctagtag tgaacggtgt ggacttgctg agctcgcgct cggaccagaa ccgccgagag
     2701 caccacacag acgagtatga gtacgacgag ctgatagtgc gccgcgggca gcctttccat
     2761 atgctcctcc tcctgtcccg gacctatgaa tcctctgatc gcatcaccct tgagttactc
     2821 atcggaaaca accccgaggt gggcaagggc acgcacgtga tcatcccagt gggcaagggg
     2881 ggcagtggag gctggaaagc ccaggtggtc aaggccagtg ggcagaatct gaacctgcgg
     2941 gtccacactt cccccaacgc catcatcggc aagtttcagt tcacagtccg cacacaatca
     3001 gacgctgggg agttccagtt gccctttgac ccccgcaatg agatctacat cctcttcaac
     3061 ccctggtgcc cagaggacat tgtgtacgtg gaccatgagg attggcggca ggagtatgtt
     3121 cttaatgagt ctgggagaat ttactacggg accgaagcac agattggtga gcggacctgg
     3181 aactacggcc agtttgacca cggggtgctg gatgcctgct tatacatcct ggaccggcgg
     3241 gggatgccat atggaggccg tggagaccca gtcaatgtct cccgggtcat ctctgccatg
     3301 gtgaactccc tggatgacaa tggagtcctg attgggaact ggtctggtga ttactcccga
     3361 ggcaccaacc catcagcgtg ggtgggcagc gtggagatcc tgcttagcta cctacgcacg
     3421 ggatattccg tcccctatgg ccagtgctgg gtctttgctg gcgtgaccac cacagtgctg
     3481 cgctgcctgg gtctggccac ccgtactgtc accaacttca actccgccca cgacacagac
     3541 acatccctta ccatggacat ctacttcgac gagaacatga agcccctgga gcacctgaac
     3601 catgattctg tctggaactt ccatgtgtgg aacgactgct ggatgaagag gccggatctg
     3661 ccctcgggct ttgatgggtg gcaggtggtg gatgccacac cccaagagac tagcagtggc
     3721 atcttctgct gcggcccctg ctctgtggag tccatcaaga atggcctggt ctacatgaag
     3781 tacgacacgc ctttcatttt tgctgaggtg aatagtgaca aggtgtactg gcagcggcag
     3841 gatgatggca gcttcaagat tgtttatgtg gaggagaagg ccatcggcac actcattgtc
     3901 acaaaggcca tcagctccaa catgcgggag gacatcacct acctctataa gcacccagaa
     3961 ggctcagacg cagagcggaa ggcagtagag acagcagcag cccacggcag caaacccaat
     4021 gtgtatgcca accggggctc agcggaggat gtggccatgc aggtggaggc acaggacgcg
     4081 gtgatggggc aggatctgat ggtctctgtg atgctgatca atcacagcag cagccgccgc
     4141 acagtgaaac tgcacctcta cctctcagtc actttctata ctggtgtcag tggtaccatc
     4201 ttcaaggaga ccaagaagga agtggagctg gcaccagggg cctcggaccg tgtgaccatg
     4261 ccagtggcct acaaggaata ccggccccat cttgtggacc agggggccat gctgctcaat
     4321 gtctcaggcc acgtcaagga gagcgggcag gtgctggcca agcagcacac cttccgtctg
     4381 cgcaccccag acctctccct cacgttactg ggagcagcag tggttggcca ggagtgtgaa
     4441 gtacagattg tcttcaagaa cccccttccc gtcaccctca ccaatgtcgt cttccggctc
     4501 gaaggctctg ggttacagag gcccaagatc ctcaacgttg gggacattgg aggcaatgaa
     4561 acagtgacac tgcgccagtc gtttgtgcct gtgcgaccag gcccccgcca gctcattgcc
     4621 agcttggaca gcccacagct ctcccaggtg cacggtgtca tccaggtgga tgtggcccca
     4681 gcccctgggg atgggggctt cttctcagac gctggaggtg acagtcactt aggagagacc
     4741 atccctatgg catctcgagg tggagctgga tccgactaca aagaccatga cggtgattat
     4801 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc
     4861 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4921 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4981 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     5041 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     5101 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5161 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5221 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5281 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5341 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5401 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5461 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5521 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5581 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5641 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5701 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5761 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5821 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5881 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     5941 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     6001 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     6061 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6121 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6181 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6241 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6301 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6361 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6421 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6481 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6541 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6601 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6661 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6721 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6781 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6841 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6901 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6961 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7021 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7081 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7141 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7201 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7261 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7321 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
     7381 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7441 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7501 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7561 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7621 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7681 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7741 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7801 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7861 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7921 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7981 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8041 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     8101 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8161 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8941 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9061 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10201 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10261 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10321 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10381 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10441 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10501 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10561 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10621 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10681 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
