LOCUS       V039260                10363 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039260
VERSION     V039260
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4389
                     /label="TBC1D15(NM_022771)"
                     /note="TBC1D15(NM_022771)"
                     /gene="TBC1D15"
     CDS             4396..4461
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4505..4716
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4729..4997
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5026..5035
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5056..5718
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5788..5841
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5842..6441
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6442..7030
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7104..7337
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7409..7530
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7570..7705
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7738..7754)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7762..7778
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7786..7816)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7831..7852
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8140..8728)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8899..9759)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9760..9864)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10338..10354
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cggcggcggg tgttgtgagc
     2341 gggaagatta tatatgaaca agaaggagta tatattcact catcttgtgg aaagaccaat
     2401 gaccaagacg gcttgatttc aggaatatta cgtgttttag aaaaggatgc cgaagtaata
     2461 gtggactgga gaccattgga tgatgcatta gattcctcta gtattctcta tgctagaaag
     2521 gactccagtt cagttgtaga atggactcag gccccaaaag aaagaggtca tcgaggatca
     2581 gaacatctga acagttacga agcagaatgg gacatggtta atacagtttc atttaaaagg
     2641 aaaccacata ccaatggaga tgctccaagt catagaaatg ggaaaagcaa atggtcattc
     2701 ctgttcagtt tgacagacct gaaatcaatc aagcaaaaca aagagggtat gggctggtcc
     2761 tatttggtat tctgtctaaa ggatgacgtc gttctccctg ctctacactt tcatcaagga
     2821 gatagcaaac tactgattga atctcttgaa aaatatgtgg tattgtgtga atctccacag
     2881 gataaaagaa cacttcttgt gaattgtcag aataagagtc tttcacagtc ttttgaaaat
     2941 cttcttgatg agccagcata tggtttaata caagcaggac tgctagacag aagaaagctg
     3001 ttgtgggcca ttcaccactg gaaaaaaatt aaaaaggacc cttatacggc aactatgata
     3061 ggattttcca aagtcacaaa ctacattttt gacagtttga gaggcagcga tccctctaca
     3121 catcaacgac caccttcaga aatggcagat tttcttagtg atgctattcc aggtctaaag
     3181 ataaatcaac aagaagaacc aggatttgaa gtcatcacaa gaattgattt gggggaacgc
     3241 cctgttgttc aaaggagaga accggtatca ctggaagaat ggactaagaa cattgattct
     3301 gaaggaagaa ttttaaatgt agataatatg aagcagatga tatttagagg gggacttagt
     3361 catgcattga gaaagcaagc atggaaattt cttctgggtt attttccctg ggacagtacc
     3421 aaggaggaaa gaacccaatt acaaaagcaa aaaactgatg aatacttcag aatgaaactg
     3481 cagtggaaat ccatcagcca ggaacaagag aaaagaaatt cgaggttaag agattataga
     3541 agtcttatcg aaaaagatgt taacagaaca gatcgaacaa acaagtttta tgaaggccaa
     3601 gataatccag ggttgatttt acttcatgac attttgatga cctactgtat gtatgatttt
     3661 gatttaggat atgttcaagg aatgagtgat ttactttccc ctcttttata tgtgatggaa
     3721 aatgaagtgg atgccttttg gtgctttgcc tcttacatgg accaaatgca tcagaatttt
     3781 gaagaacaaa tgcaaggcat gaagacccag ctaattcagc tgagtacctt acttcgattg
     3841 ttagacagtg gattttgcag ttacttagaa tctcaggact ctggatacct ttatttttgc
     3901 ttcaggtggc ttttaatcag attcaaaagg gaatttagtt ttctagatat tcttcgatta
     3961 tgggaggtaa tgtggaccga actaccatgt acaaatttcc atcttcttct ctgttgtgct
     4021 attctggaat cagaaaagca gcaaataatg gaaaagcatt atggcttcaa tgaaatactt
     4081 aagcatatca atgaattgtc catgaaaatt gatgtggaag atatactctg caaggcagaa
     4141 gcaatttctc tacagatggt aaaatgcaag gaattgccac aagcagtctg tgagatcctt
     4201 gggcttcaag gcagtgaagt tacaacacca gattcagacg ttggtgaaga cgaaaatgtt
     4261 gtcatgactc cttgtcctac atctgcattt caaagtaatg ccttgcctac actctctgcc
     4321 agtggagcca gaaatgacag cccaacacag ataccagtgt cctcagatgt ctgcagatta
     4381 acacctgcag gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4441 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4501 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4561 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4621 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4681 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4741 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4801 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4861 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4921 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4981 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5041 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5101 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5161 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5221 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5281 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5341 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5401 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5461 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5521 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     5581 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     5641 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     5701 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     5761 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     5821 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     5881 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5941 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6001 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6061 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6121 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6181 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6241 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6301 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6361 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6421 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6481 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6541 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6601 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6661 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6721 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6781 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6841 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6901 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6961 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7021 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7081 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7141 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7201 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7261 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7321 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7381 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7681 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     7741 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7801 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7861 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7921 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7981 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8041 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8101 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8161 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8221 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8281 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8341 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8401 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8461 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8521 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8581 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8641 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8701 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8761 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8821 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8881 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9001 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9061 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9181 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9721 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9781 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9841 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     9901 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     9961 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10021 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10081 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10141 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10201 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10261 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10321 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
