LOCUS       V042636                12251 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042636
VERSION     V042636
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6336
                     /label="Syngap1(NM_001281491)"
                     /note="Syngap1(NM_001281491)"
                     /gene="Syngap1"
     CDS             6343..6366
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        6410..6621
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6634..6902
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6931..6940
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6961..7623
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7693..7746
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7747..8346
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8347..8934
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9008..9241
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9313..9434
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9453..9588
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9626..9642)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9650..9666
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9674..9704)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9719..9740
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10028..10616)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10787..11647)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11648..11752)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12226..12242
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gcaggtctcg agcctccatc
     2341 catcggggga gcatccccgc gatgtcctat gcccccttca gagatgtacg gggaccccct
     2401 atgcaccgaa cccaatacgt tcattccccg tacgatcgtc ctggctggaa ccctcggttc
     2461 tgcatcatct cggggaacca gctgctcatg ctggatgagg atgagataca cccccttctg
     2521 atccgcgacc ggaggagcga gtccagccga aacaaactgc tgagacgcac cgtctctgtg
     2581 ccagtggagg ggcggcccca cggcgagcat gaataccact tgggtcgctc gaggaggaag
     2641 agtgtcccag gggggaaaca gtacagcatg gaggccgccc ccgctgcgcc cttccggccc
     2701 tcgcaaggct tcctgagccg gaggctaaaa agctctatca aacgtacaaa gtcacaaccc
     2761 aaacttgacc ggaccagcag ctttcgacag atcctgcctc gcttccgaag tgctgaccat
     2821 gaccgggccc ggctgatgca gagcttcaag gagtcacatt cccacgagtc cctgctgagt
     2881 cccagtagtg ctgctgaggc cttggagctc aacctggatg aagactccat tatcaagcca
     2941 gtacacagct ccatcctggg tcaggagttc tgctttgagg taacaacatc atctggaaca
     3001 aaatgctttg cctgtcggtc tgcagctgaa agggacaaat ggattgagaa tctgcagagg
     3061 gctgtaaaac ccaacaagga caacagccgc cgagtagata acgtgctgaa gctatggatc
     3121 atagaggctc gagagctgcc ccccaagaag agatattact gtgagctgtg cctggacgac
     3181 atgctgtatg cacgaaccac ctccaagccc cgctcggctt caggagacac cgtcttttgg
     3241 ggcgagcact ttgagtttaa caacctgcct gccgtccggg cccttcggct gcatctgtac
     3301 cgtgactcag acaaaaagcg gaagaaggac aaggctggct acgttggcct ggtgactgtt
     3361 ccagtggcca ccctagctgg gcgccacttc acagagcagt ggtaccccgt gaccttgccg
     3421 acaggcagtg ggggctctgg gggcatgggc tcggggggag gaggagggtc agggggtggc
     3481 tcagggggca aagggaaagg aggttgtcct gctgtgagac tgaaagcccg ttaccagacg
     3541 atgagcatcc tgcccatgga gctgtataag gagtttgcag agtatgtgac caaccattac
     3601 cggatgctat gtgcagtgct ggagcctgcc ctgaatgtca aaggcaagga agaggtggcc
     3661 agtgcactgg ttcacatcct gcagagcaca ggcaaggcca aggacttcct ttcagacatg
     3721 gccatgtcag aggtagaccg attcatggag cgggaacacc tcatattccg agagaacacg
     3781 ctcgccacta aagccataga agagtatatg agactgattg gccagaaata cctcaaggat
     3841 gccattgggg agttcattcg tgctctgtat gaatctgagg agaactgtga agtagacccc
     3901 atcaagtgca cagcgtccag tctggcagag caccaggcca acctgcggat gtgctgtgag
     3961 ttggccctgt gcaaggtggt caactcccat tgcgtgttcc cgagggagct gaaggaggtg
     4021 tttgcatctt ggcggctgcg ctgcgcagag cggggccgag aggacattgc tgacaggctg
     4081 atcagcgcct cgctcttcct gcgcttcctc tgcccggcca ttatgtcgcc cagtctattt
     4141 gggcttatgc aggagtaccc agatgagcag acctcacgaa ccctcaccct catcgccaag
     4201 gtcatccaga acctggccaa cttttccaag tttacctcaa aggaggactt cctgggcttc
     4261 atgaatgagt ttctggagct ggagtggggc tctatgcagc agttcttgta tgagatatcc
     4321 aacctggaca ccttgaccaa cagcagcagt tttgagggct atatagactt gggccgcgag
     4381 ctctccacac ttcacgccct gctctgggag gtgctgcccc agctcagcaa ggaagccctc
     4441 ctgaagctgg gcccactgcc ccggctcctc aatgacatca gcacagccct gaggaaccct
     4501 aacatccaaa ggcagccaag ccgccagagt gaacggactc ggtctcagcc catggtgctg
     4561 cgagggccat cagccgagat gcagggctac atgatgcggg acctcaatag ctccatcgac
     4621 cttcaatcct tcatggctcg aggcctcaac agctctatgg acatggctcg cctcccctcc
     4681 ccaaccaagg aaaaaccacc accaccacca cccggtgggg gtaaagacct tttctatgtg
     4741 agccggccac cactggcccg gtcctcccca gcatactgca cgagcagctc ggacatcaca
     4801 gagccagagc agaagatgct gagtgtcaac aagagtgtgt ccatgctgga ccttcagggc
     4861 gacgggcctg gaggtcgcct taacagtagc agtgtttcca acctggcagc tgttggggac
     4921 ttgttgcatt ccagccaggc ctcgctgaca gcagctttgg ggttgcggcc tgcacctgcc
     4981 gggcgcctct cccaggggag tggctcttcc atcacagcag ctggcatgcg cctcagccag
     5041 atgggggtca ctacagatgg tgtccccgcc cagcaactgc gcatccccct ttccttccag
     5101 aaccctctct tccatatggc tgctgatggg ccagggcccc cagcaggcca tggagggagc
     5161 agtggtcatg gtccaccttc ctcccatcac caccaccacc accatcacca ccaccgaggg
     5221 ggagaacccc caggggacac ttttgcccca ttccatggct atagcaagag cgaggacctc
     5281 tcttcagggg tccctaagcc ccccgccgcc tccatccttc acagccacag ctacagcgac
     5341 gagtttggac cctctggcac tgattttacc cgccggcagc tctcgcttca ggacagtcta
     5401 cagcacatgc tctcccctcc ccagattacc atcggtcccc agaggccagc tccctcaggg
     5461 ccgggagggg gcagcggcgg gggcagcggt gggggccagc cacccccctt gcagaggggc
     5521 aagtctcaac agttgacagt gagtgccgcc cagaaacctc ggccgtctag cgggaacctg
     5581 ttgcagtccc cggagccaag ctacggacct gcccgtcctc ggcagcagag cctcagcaaa
     5641 gagggcagca ttgggggcag cgggggcagc gggggcggag ggggtggggg gctcaagccc
     5701 tccatcacca agcagcattc ccagactcca tccacgctta accccacaat gccagcctcg
     5761 gagcggaccg tagcctgggt ctccaacatg cctcacctgt cggctgacat cgagagtgca
     5821 cacatcgagc gagaagagta caagctcaag gagtactcca agtccatgga cgaaagccgg
     5881 ctggacaggg tgaaggagta tgaggaggag atacattcgc tgaaggagag gctacacatg
     5941 tccaaccgga agctggaaga gtatgagcgg aggttgctgt cccaggaaga acagaccagc
     6001 aagatcctga tgcagtacca agcccgcctg gagcagagtg agaagcgctt gagacagcag
     6061 caggtggaga aggactccca gatcaagagc atcattggca ggctgatgct ggtggaggag
     6121 gagctgcgtc gggaccaccc cgccatggct gagccgctgc ctgagcccaa gaagaggctg
     6181 ctcgacgctc aggagaggca gcttcccccc ttgggtccaa caaacccgcg tgtgacgctg
     6241 gccccacctt ggaacggcct ggccccccca gccccacccc ccccaccccg gctgcagatc
     6301 acagagaacg gcgagttccg gaacaccgca gaccacggat cccaccatca ccatcaccat
     6361 caccattagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     6421 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     6481 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     6541 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     6601 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     6661 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     6721 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     6781 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     6841 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     6901 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg
     6961 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     7021 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     7081 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     7141 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     7201 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     7261 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     7321 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     7381 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     7441 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     7501 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     7561 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     7621 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     7681 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     7741 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     7801 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     7861 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     7921 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     7981 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8041 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     8101 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     8161 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8221 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8281 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8341 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8401 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8461 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8521 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8581 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8641 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8701 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     8761 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8821 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8881 cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta
     8941 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     9001 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     9061 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9121 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9181 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9241 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     9301 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9421 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     9481 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9541 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     9601 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10441 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    10501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11761 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    11821 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    11881 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11941 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    12001 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    12061 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    12121 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    12181 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    12241 gtgccaagct g
//
