LOCUS       V027701                10625 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027701
VERSION     V027701
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4626
                     /label="Stat3(NM_012747)"
                     /note="Stat3(NM_012747)"
                     /gene="Stat3"
     CDS             4633..4659
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4663..4689
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4693..4719
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4784..4995
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5008..5276
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5305..5314
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5335..5997
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6067..6120
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6121..6720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6721..7308
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7382..7615
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7687..7808
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7827..7962
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8000..8016)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8024..8040
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8048..8078)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8093..8114
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8402..8990)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9161..10021)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10022..10126)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10600..10616
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ctcagtggaa ccagctccag
     2341 cagctggaca cgcgctacct ggagcagctt catcagctgt acagcgatag cttccccatg
     2401 gagctgcggc agttcctggc gccttggatt gagagccaag attgggcata tgcagccagc
     2461 aaagagtcac acgccactct ggtgtttcat aacctcttgg gcgagatcga ccagcagtat
     2521 agccgattcc tgcaggagtc caatgtcctc tatcagcaca acctgcgaag aatcaagcag
     2581 ttcctgcaga gcaggtatct tgagaagcca atggaaattg cccggattgt ggcccgatgc
     2641 ctgtgggaag agtctcgcct cctccagacg gcagccacgg cagcccagca agggggccag
     2701 gccaaccacc ccacagctgc cgtagtgacg gagaagcagc agatgctgga acagcatctt
     2761 caggatgtcc ggaagcgtgt gcaggatcta gaacagaaaa tgaaagtggt ggagaatctc
     2821 caggatgact ttgatttcaa ctataaaacc ctcaagagtc aaggagacat gcaggatctg
     2881 aatggaaaca accagtctgt gaccagacag aagatgcagc agctggagca gatgctcacg
     2941 gccctggacc agatgcggag gagcatcgtg agcgagctgg cagggctctt gtcagcaatg
     3001 gagtacgtgc agaagacact gaccgatgaa gagctggctg actggaagag gcggcagcag
     3061 atagcgtgca tcggaggccc tcccaacatc tgcctggacc gtctggaaaa ctggataact
     3121 tcattagcag aatctcaact tcagacccgc caacaaatta agaaactgga ggagctgcag
     3181 cagaaagtgt cctacaaggg ggaccctatt gtgcagcacc ggccaatgct ggaggagagg
     3241 atcgtggatc tgttcagaaa cttaatgaag agtgccttcg tggtggagcg gcagccctgt
     3301 atgcccatgc acccggaccg gcccttagtc atcaagactg gtgtccagtt taccacaaaa
     3361 gtcaggttgc tggtcaaatt tcctgagttg aattatcagc ttaaaattaa agtgtgcatt
     3421 gataaggact ctggggatgt tgctgccctc agagggtctc ggaaatttaa cattctgggc
     3481 acgaacacaa aggtgatgaa catggaggag tccaacaacg gcagcctgtc tgcagagttc
     3541 aagcacctga ccctgaggga gcagagatgt gggaatgggg gccgtgccaa ttgtgatgcc
     3601 tccttgattg tcactgagga gctgcacctg atcacctttg agacagaggt gtaccaccaa
     3661 ggtctcaaga tcgacctaga gacccactcc ttgccagtcg tggtgatctc caacatctgt
     3721 cagatgccta atgcttgggc atcaatcctg tggtataaca tgctgaccaa taaccccaag
     3781 aacgtgaact tcttcactaa gcctccgatt ggaacctggg accaagtggc cgaggtgctg
     3841 agctggcagt tctcgtccac caccaagcga gggctgagca tcgagcagct gaccacgctg
     3901 gccgagaagc tcttagggcc tggtgtgaac tactcagggt gtcagatcac atgggctaag
     3961 ttttgcaaag aaaacatggc cggcaagggc ttctcgttct gggtctggct agacaatatc
     4021 atcgaccttg tgaaaaagta tatcttggcc ctttggaatg aagggtacat catgggtttc
     4081 atcagcaagg agcgggagag ggccatccta agcacaaagc ccccgggcac cttcctgctg
     4141 cggttcagtg agagcagcaa ggaaggaggg gtcactttca cttgggtgga aaaggacatc
     4201 agtggcaaga cccagatcca gtctgtagaa ccatatacca agcagcagct gaacaacatg
     4261 tcatttgctg aaatcatcat gggctataag atcatggacg ctaccaacat cctggtatcc
     4321 ccactggtct acctctaccc tgacattccc aaggaggagg cattcggaaa gtattgtcgc
     4381 cccgagagcc aggagcaccc tgaagctgac ccaggtagtg ctgcccctta cctgaagacc
     4441 aagttcatct gtgtgacacc aacgacctgc agcaatacca ttgacctgcc gatgtccccc
     4501 cgcactttag attcattgat gcagtttgga aataacgggg aaggcgctga gccctcagca
     4561 ggagggcagt ttgagtcgct cacgtttgac atggatctga cctcggagtg tgctacctcc
     4621 ccgatgctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct
     4681 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccct gcgatatcca
     4741 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4801 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4861 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4921 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4981 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5041 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5101 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5161 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5221 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5281 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     5341 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     5401 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     5461 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5521 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5581 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5641 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5701 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5761 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5821 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5881 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5941 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     6001 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     6061 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     6121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6841 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6901 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6961 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7021 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7081 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7141 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7201 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc
     7261 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta
     7321 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7381 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     7441 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7501 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7561 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7621 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7681 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7741 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7801 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     7861 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7921 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     7981 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8041 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8101 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8161 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8221 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8281 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8341 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8401 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8461 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8521 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8581 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8641 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8701 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8761 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8821 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8881 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8941 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9001 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9061 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9121 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9181 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9241 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9301 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9361 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9421 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9481 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9541 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9601 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9661 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9721 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9781 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9841 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9901 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9961 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10021 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10081 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10141 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10201 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    10261 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10321 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10381 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10441 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10501 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    10561 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    10621 agctg
//
