LOCUS       V014251                12013 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014251
VERSION     V014251
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2011..2214
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2303..6121
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus 2. Accession#: P0DTC2"
     promoter        6172..6383
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             6396..6664
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6720..7385
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             7455..7508
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             7509..8105
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    8109..8696
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8770..9003
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9075..9209
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      9215..9350
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(9436..9466)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9481..9502)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9790..10378)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10552..11409)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11410..11514)
                     /label="AmpR promoter"
     primer_bind     11988..12004
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtttgt ttttcttgtt ttattgccac tagtctctag
     2341 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac
     2401 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga
     2461 cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac
     2521 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc
     2581 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa
     2641 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt
     2701 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat
     2761 ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca
     2821 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt
     2881 gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt
     2941 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat
     3001 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga
     3061 ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag
     3121 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact
     3181 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta
     3241 tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac
     3301 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg
     3361 gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc
     3421 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac
     3481 taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg
     3541 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt
     3601 tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta
     3661 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta
     3721 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca
     3781 atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact
     3841 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt
     3901 ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac
     3961 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac
     4021 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg
     4081 tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca
     4141 ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg
     4201 gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc
     4261 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag
     4321 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat
     4381 tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc
     4441 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa
     4501 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt
     4561 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga
     4621 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc
     4681 aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag
     4741 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt
     4801 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca
     4861 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata
     4921 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc
     4981 attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca
     5041 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa
     5101 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa
     5161 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat
     5221 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat
     5281 tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat
     5341 tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt
     5401 acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc
     5461 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa
     5521 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg
     5581 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca
     5641 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt
     5701 caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga
     5761 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa
     5821 tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt
     5881 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc
     5941 atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat
     6001 gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg
     6061 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac
     6121 ataaggatcc gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc
     6181 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct
     6241 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     6301 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     6361 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg
     6421 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg
     6481 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac
     6541 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt
     6601 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca
     6661 gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc
     6721 tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc
     6781 tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca
     6841 ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct
     6901 accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg
     6961 gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct
     7021 tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct
     7081 tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc
     7141 acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc
     7201 gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc
     7261 accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc
     7321 acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct
     7381 tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct
     7441 ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     7501 ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7561 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7621 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7681 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7741 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7801 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7861 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     7921 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     7981 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8041 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     8101 gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8161 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8221 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8281 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8341 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8401 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8461 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     8521 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8581 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8641 cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct ccccgcctgg
     8701 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8761 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta
     8821 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8881 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8941 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9001 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9061 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9181 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
     9241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9301 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg
     9361 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9421 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9481 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10201 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    10261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11521 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
    11581 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
    11641 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
    11701 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
    11761 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    11821 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
    11881 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    11941 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12001 cagtgccaag ctg
//
