LOCUS       V038988                10574 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038988
VERSION     V038988
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4659
                     /label="Sp1(NM_013672)"
                     /note="Sp1(NM_013672)"
                     /gene="Sp1"
     CDS             4666..4689
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHHHH"
                     /label="8xHis"
     promoter        4733..4944
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4957..5225
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5254..5263
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5284..5946
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6016..6069
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6070..6669
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6670..7257
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7331..7564
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7636..7757
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7776..7911
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7949..7965)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7973..7989
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7997..8027)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8042..8063
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9110..9970)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9971..10075)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10549..10565
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gcgaccaaga tcactccatg
     2341 gatgaagtga cagctgtggt gaagattgaa aaagatgttg gtggcaataa tgggggtagc
     2401 ggcaatggtg gcggtgccgc cttttctcag actcgaagca gcagcacagg cagtagcagc
     2461 agcagtggtg gcggaggagg gcaggaatcc cagccatctc ctttggctct gctggcagca
     2521 acctgcagca gaattgagtc acccaatgag aacagcaaca actcccaggg tccgagtcag
     2581 tcagggggca caggtgaact tgacctcaca gccgcacaac tttcacaggg tgccaatggc
     2641 tggcagatca tctcttcctc ctctggggct acccctacct caaaggaaca gagtggcaac
     2701 agtaccaatg gcagcgagtc ttccaagaac cgcacagtct ctggtgggca gtatgttgtg
     2761 gctgctaccc ccaacttaca gaaccagcaa gttctgacag gtctccctgg agtaatgcct
     2821 aatattcagt atcaagtaat cccacagttc cagactgttg atgggcagca gctgcagttt
     2881 gctgccactg gggcccaagt gcagcaggat ggttctggtc aaatacagat cataccaggt
     2941 gcaaaccaac agatcatccc aaatagagga agtgggggca acattattgc tgctatgcca
     3001 aatctactcc agcaggctgt cccccttcaa ggccttgcta ataatgtgct ctcaggacag
     3061 actcagtatg tgaccaatgt accagtggcc ctgaatggga acatcacctt gctacctgtc
     3121 aacagcgttt ctgcagctac cctgactccc agctctcagg caggcactat cagcagctct
     3181 ggatcccagg agagcagctc acagcctgtc acctcaggga ctgccatcag ttctgccagc
     3241 ttggtgtcat cacaagctag ttccagctcc ttttttacca atgccaatag ttattcaaca
     3301 actactacca ccagcaacat gggaattatg aactttacca gcagtggctc atcagggact
     3361 agttctcaag gccagacgcc ccagagggtt ggtgggctac aagggtctga ttctctgaac
     3421 atccagcaga accagacatc aggaggctcg ctgcaaggaa gtcagcagaa agagggagag
     3481 caaagtcagc agacacagca acaacaaatc cttattcagc ctcagctagt tcaaggagga
     3541 caagctcttc aggcccttca agcagcacca ttgtccggac agaccttcac aactcaagct
     3601 atttcccagg aaacccttca gaacctccag cttcaggctg ttcaaaactc tggtcccatc
     3661 atcattcgga caccaaccgt ggggcccaat ggacaggtca gttggcagac ccttcagctg
     3721 cagaatcttc aagttcagaa cccacaagcc cagacaatca ccttggcccc tatgcagggt
     3781 gtttccttgg ggcagactag cagcagtaat accaccctaa cacccattgc ctcagctgcc
     3841 tccattcctg ctggcacagt cactgtgaat gctgctcaac tctcctccat gccaggcctc
     3901 cagaccatta acctcagtgc attgggtact tcagggatcc aggtgcacca gcttccaggc
     3961 ctgcctttgg ctatagcaaa caccccaggt gatcatggaa ctcaacttgg tcttcatgga
     4021 tctggtggtg atgggataca tgatgagaca gcaggtggag aaggagagaa tagctctgat
     4081 ctccaacccc aagctggacg caggactcgt cgggaagcat gtacatgccc ctattgcaaa
     4141 gacagtgagg gaagagcctc aggagatcct ggcaaaaaga aacagcacat ttgtcacatc
     4201 caaggatgcg gcaaagtata tggcaagacc tcacatctcc gagcacactt gcgctggcat
     4261 acaggggaga ggccattcat gtgtaattgg tcatattgtg ggaagcgctt tacacgttcg
     4321 gacgagcttc agagacataa acgtacacat acaggagaga agaaatttgc ctgccctgag
     4381 tgccctaagc gtttcatgag gagtgatcac ctgtcaaagc atatcaagac tcaccagaac
     4441 aagaagggag gcccaggtgt agccctgagt gtgggcacat tgcccctgga cagtggggca
     4501 ggttcagaag gcactgccac tccttcagcc cttattacca ccaatatggt agccatggag
     4561 gccatctgtc cagagggtat tgcccgtctt gccaacagtg gcatcaacgt catgcaggtg
     4621 acagagctgc agtccattaa tatcagtggc aatggtttcg gatcccacca tcaccatcac
     4681 catcaccatt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4741 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4801 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4861 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4921 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4981 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5041 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5101 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5161 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5221 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5281 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5341 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5401 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5461 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     5521 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     5581 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     5641 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     5701 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     5761 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     5821 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     5881 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     5941 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6001 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6061 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6121 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6181 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6241 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6301 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6361 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6421 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6481 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6541 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6601 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6661 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6721 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6781 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6841 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6901 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6961 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7021 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7081 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7141 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7201 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     7261 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7321 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7381 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7441 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7501 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7561 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7621 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7741 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7801 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7861 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7921 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8761 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10081 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10141 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10321 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10381 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10561 ccagtgccaa gctg
//
