LOCUS       V044020                11877 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044020
VERSION     V044020
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5959
                     /label="Sorcs3(NM_025696)"
                     /note="Sorcs3(NM_025696)"
                     /gene="Sorcs3"
     CDS             5966..6031
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6075..6286
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6299..6567
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6602..7309
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             7319..7372
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7373..7972
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7973..8560
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8634..8867
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8939..9060
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9079..9214
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9252..9268)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9276..9292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9300..9330)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9345..9366
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9654..10242)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10413..11273)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11274..11378)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11852..11868
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggaggc ggcaggcact gagcgccccg caggctggcc
     2341 cggggcaccc ctcgcccgga ctgggctgct gctcttgtca acatgggtcc tggccggcgc
     2401 cgagatcact tggggcgcga caggaggtcc ggggcgcctg gtgtccccgg cttcacgacc
     2461 accagtgttg cctcctctct tgccacgggc agcggagaac cggtggcctg aggagctggc
     2521 atctgcacgg agagccgctg caccgagacg ccggtccagg ctagagccac tatcccaggc
     2581 gagccgcggg gagatacgga ctgaagctgc gggaatgtcg cctgaaggcg ctaggtgggt
     2641 cccgggcatt ccatctccta gccaggcggg cagtgcgagg aggactcgcc gggcgcagcc
     2701 cccaagcccc ctggagcgcg gggactcttg ggctactgct ctcgcagatg gtgccaaagg
     2761 aagccgtcct cacaccaagg gttcgcgaga ggaggtgagg gcgacgcgga ctggaggagc
     2821 gtccactgag gagctccggc tgcccagcac gtcttttgca ctgaccgggg actcggctca
     2881 taaccaagcc atggtgcact ggtcgggaca caacagcagt gtcatactga tcctgacgaa
     2941 gctctatgac ttcaacctgg ggagtgtgac tgagagttct ctgtggaggt cagtagatta
     3001 tggcgccacc tatgaaaaac ttaatgacaa agtgggtttg aagaccgtcc tcagttatct
     3061 ctatgtcaac cccaccaaca aaaggaagat catgctcctt agtgaccccg agatggagag
     3121 cagtgtactg atcagctcag atgaaggggc gacctaccaa aagtaccggc ttacattcta
     3181 catccagagc ctgctcttcc atcccaagca ggaggactgg gtgctggcct acagcttgga
     3241 ccaaaagctt tatagctcca tggacttcgg cagacgatgg caactcatgc atgaacgcat
     3301 cacgcccaac aggttttact ggtcggtgtc tggactggat aaggaggcag acctggtgca
     3361 catggaagtg aggacggcag atggatatgc ccactacctc acctgcagaa tccaagaatg
     3421 tgctgagaca actcggagcg gcccttttgc ccgctccatt gacatcagct ccttggttgt
     3481 ccaggatgaa tatatcttca ttcaggtaac aataggtgga cgagccagct actacgtgtc
     3541 gtatcgaaga gaagcctttg cccagataaa gctgcccaag tactccttgc caaaggacat
     3601 gcacattatc agtaccgatg agaaccaagt gtttgcagcc gtccaagaat ggaaccagaa
     3661 tgacacctac aacctctaca tctcagacac acgtgggatc tactttaccc tggctatgga
     3721 gaacattaag agcagccgag gtctaatggg aaacatcatt attgaattat atgaggtagc
     3781 aggtatcaag gggatattcc tggcaaacaa gaaggtggat gaccaggtga agacatacat
     3841 cacttacaac aaaggcaggg attggcgtct cctgcaagct ccagatgtgg acctgagagg
     3901 aagcccagtg cactgtttac tgcccttctg ttccctacac ttgcatctgc agctctctga
     3961 aaacccatat tcctctggaa gaatctccag caaggacaca gcgcctggac ttgtggttgc
     4021 cacgggtaat attggctccg aactctccta tacggatatc ggtgtgttca tctcctctga
     4081 tgggggcaac acatggagac agatctttga tgaagaatac aatgtctggt tcctagactg
     4141 gggtggtgct ctggtggcca tgaagcacac acctctgcca gtcaggcatt tgtgggtgag
     4201 ttttgatgag ggccattcct gggacaagta tggtttcaca ttgctgcctc ttttcgtgga
     4261 tggggcgttg gtggaggcag gagtagagac gcacatcatg acagtttttg gccacttcag
     4321 cctgcgctcc gaatggcagt tggtgaaagt ggactacaag tctatcttca gccggcgctg
     4381 taccaaggag gactttgaga cttggcacct gctcaatcag ggagagcctt gtgtcatggg
     4441 ggaaaggaaa atattcaaga aacgcaagcc aggcgctcag tgtgcccttg gcagagagta
     4501 ctctgggtcg gtggtgtcag aaccctgtgt ctgtgcggac tgggacttcg aatgtgacta
     4561 tggctatgag agacatggag agagccagtg tgtcccagct ttctggtaca acccagcttc
     4621 cccttcaaag gactgcagcc ttggtcaaag ctaccttaac agcactgggt accggaggat
     4681 tgtatccaac aactgcacag atgggctaag ggataagtac tctgctaaga ctcagttgtg
     4741 tcctggaaaa gctcctcggg gcctccatgt ggtgaccact gacgggcgcc tggtggcaga
     4801 acagggccac aatgcaacct tcatcattct aatggaggag ggtgaccttc aaaggacaaa
     4861 catccagctt gactttggag atggggtggc tgtatcctat gccaacttca gccccattga
     4921 ggatggcatc aggcatgtgt ataagagcgc ggggatcttc caggtgacag cttatgcgga
     4981 gaacaacctt ggctcggaca cagccttcct cttcctgcat gtggtttgtc cagtggaaca
     5041 cgttcacctt cgagtcccat tcgtcgcaat acggaataag gacgtcaaca tcagtgcagt
     5101 tgtgtggccc agtcagctag ggaccctcac ctacttctgg tggtttggaa atagcacaaa
     5161 gcccctcatc accttggaca gcagcatctc tttcacattc cttgcagagg gaaccaacac
     5221 catcactgtc caggttgctg ctgggaatgc cctcatccaa gacacaaaag agattgcagt
     5281 acatgaatac ttccagtcac agctcttatc gttctctcca aatctggatt accacaatcc
     5341 tgatatccct gagtggaggc aagacattgg caatgtcatc aagcgagctc tgattaaagt
     5401 gaccagtgtc ccagaagacc agatcctcgt ggctgtgttc ccgggtctcc ctacttcagc
     5461 agagcttttc attctcccac ctaagaactt gacagagagg aggaaaggcc acgaagggga
     5521 tctcgaacaa attgtagaga cactatttaa tgcgcttaat caaaacttgg tacagtttga
     5581 gctgaagcca ggtgtccagg tcattgttta tgtcacacag ctgacattag ctcccctggt
     5641 ggactccagc gctgggcata gcagctctgc catgcttatg ctcctctcgg tggtattcgt
     5701 cggcttggct gtgtttctga tctacaagtt taaaaggaaa atcccctgga ttaacatcta
     5761 tgcccaagtc caacatgaca aggaacagga gatgatcggg tcagtgagcc aaagtgagaa
     5821 tgcacccaaa atcacactca gtgacttcac cgagcccgag gagctgctgg acaaagagct
     5881 ggacacgcgg gtcataggaa gcattgccac cattgcaagc agcgagagca caaaggagat
     5941 ccccaactgc actagtgttg gatccgacta caaagaccat gacggtgatt ataaagatca
     6001 tgacatcgac tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc
     6061 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     6121 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     6181 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     6241 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     6301 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     6361 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     6421 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     6481 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     6541 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac
     6601 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     6661 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     6721 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     6781 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     6841 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     6901 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     6961 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     7021 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     7081 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     7141 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     7201 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     7261 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga
     7321 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga
     7381 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7441 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     7501 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7561 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7621 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7681 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7741 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7801 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7861 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7921 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc
     7981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     8041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8341 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     8401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg
     8521 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat
     8581 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8641 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt
     8701 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8761 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8821 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8881 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     9001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9061 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc
     9121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9181 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat
     9241 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9301 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9361 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9421 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9481 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9541 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9601 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9661 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9721 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9781 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9841 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9901 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9961 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10021 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10081 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10141 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10201 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10261 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10321 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10381 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10441 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    10501 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10561 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10621 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10681 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10741 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10801 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10861 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10921 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10981 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11041 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11101 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11161 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11221 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11281 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    11341 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    11401 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
    11461 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    11521 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    11581 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
    11641 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    11701 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    11761 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    11821 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
