LOCUS       V044247                11947 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044247
VERSION     V044247
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5973
                     /label="Sorcs3(NM_025696)"
                     /note="Sorcs3(NM_025696)"
                     /gene="Sorcs3"
     CDS             5980..6045
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6089..6300
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6313..6581
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6610..6619
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6640..7302
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7372..7425
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7426..8025
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8026..8614
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8688..8921
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8993..9114
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9154..9289
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9322..9338)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9346..9362
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9370..9400)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9415..9436
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9724..10312)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10483..11343)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11344..11448)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11922..11938
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggcggcagg cactgagcgc
     2341 cccgcaggct ggcccggggc acccctcgcc cggactgggc tgctgctctt gtcaacatgg
     2401 gtcctggccg gcgccgagat cacttggggc gcgacaggag gtccggggcg cctggtgtcc
     2461 ccggcttcac gaccaccagt gttgcctcct ctcttgccac gggcagcgga gaaccggtgg
     2521 cctgaggagc tggcatctgc acggagagcc gctgcaccga gacgccggtc caggctagag
     2581 ccactatccc aggcgagccg cggggagata cggactgaag ctgcgggaat gtcgcctgaa
     2641 ggcgctaggt gggtcccggg cattccatct cctagccagg cgggcagtgc gaggaggact
     2701 cgccgggcgc agcccccaag ccccctggag cgcggggact cttgggctac tgctctcgca
     2761 gatggtgcca aaggaagccg tcctcacacc aagggttcgc gagaggaggt gagggcgacg
     2821 cggactggag gagcgtccac tgaggagctc cggctgccca gcacgtcttt tgcactgacc
     2881 ggggactcgg ctcataacca agccatggtg cactggtcgg gacacaacag cagtgtcata
     2941 ctgatcctga cgaagctcta tgacttcaac ctggggagtg tgactgagag ttctctgtgg
     3001 aggtcagtag attatggcgc cacctatgaa aaacttaatg acaaagtggg tttgaagacc
     3061 gtcctcagtt atctctatgt caaccccacc aacaaaagga agatcatgct ccttagtgac
     3121 cccgagatgg agagcagtgt actgatcagc tcagatgaag gggcgaccta ccaaaagtac
     3181 cggcttacat tctacatcca gagcctgctc ttccatccca agcaggagga ctgggtgctg
     3241 gcctacagct tggaccaaaa gctttatagc tccatggact tcggcagacg atggcaactc
     3301 atgcatgaac gcatcacgcc caacaggttt tactggtcgg tgtctggact ggataaggag
     3361 gcagacctgg tgcacatgga agtgaggacg gcagatggat atgcccacta cctcacctgc
     3421 agaatccaag aatgtgctga gacaactcgg agcggccctt ttgcccgctc cattgacatc
     3481 agctccttgg ttgtccagga tgaatatatc ttcattcagg taacaatagg tggacgagcc
     3541 agctactacg tgtcgtatcg aagagaagcc tttgcccaga taaagctgcc caagtactcc
     3601 ttgccaaagg acatgcacat tatcagtacc gatgagaacc aagtgtttgc agccgtccaa
     3661 gaatggaacc agaatgacac ctacaacctc tacatctcag acacacgtgg gatctacttt
     3721 accctggcta tggagaacat taagagcagc cgaggtctaa tgggaaacat cattattgaa
     3781 ttatatgagg tagcaggtat caaggggata ttcctggcaa acaagaaggt ggatgaccag
     3841 gtgaagacat acatcactta caacaaaggc agggattggc gtctcctgca agctccagat
     3901 gtggacctga gaggaagccc agtgcactgt ttactgccct tctgttccct acacttgcat
     3961 ctgcagctct ctgaaaaccc atattcctct ggaagaatct ccagcaagga cacagcgcct
     4021 ggacttgtgg ttgccacggg taatattggc tccgaactct cctatacgga tatcggtgtg
     4081 ttcatctcct ctgatggggg caacacatgg agacagatct ttgatgaaga atacaatgtc
     4141 tggttcctag actggggtgg tgctctggtg gccatgaagc acacacctct gccagtcagg
     4201 catttgtggg tgagttttga tgagggccat tcctgggaca agtatggttt cacattgctg
     4261 cctcttttcg tggatggggc gttggtggag gcaggagtag agacgcacat catgacagtt
     4321 tttggccact tcagcctgcg ctccgaatgg cagttggtga aagtggacta caagtctatc
     4381 ttcagccggc gctgtaccaa ggaggacttt gagacttggc acctgctcaa tcagggagag
     4441 ccttgtgtca tgggggaaag gaaaatattc aagaaacgca agccaggcgc tcagtgtgcc
     4501 cttggcagag agtactctgg gtcggtggtg tcagaaccct gtgtctgtgc ggactgggac
     4561 ttcgaatgtg actatggcta tgagagacat ggagagagcc agtgtgtccc agctttctgg
     4621 tacaacccag cttccccttc aaaggactgc agccttggtc aaagctacct taacagcact
     4681 gggtaccgga ggattgtatc caacaactgc acagatgggc taagggataa gtactctgct
     4741 aagactcagt tgtgtcctgg aaaagctcct cggggcctcc atgtggtgac cactgacggg
     4801 cgcctggtgg cagaacaggg ccacaatgca accttcatca ttctaatgga ggagggtgac
     4861 cttcaaagga caaacatcca gcttgacttt ggagatgggg tggctgtatc ctatgccaac
     4921 ttcagcccca ttgaggatgg catcaggcat gtgtataaga gcgcggggat cttccaggtg
     4981 acagcttatg cggagaacaa ccttggctcg gacacagcct tcctcttcct gcatgtggtt
     5041 tgtccagtgg aacacgttca ccttcgagtc ccattcgtcg caatacggaa taaggacgtc
     5101 aacatcagtg cagttgtgtg gcccagtcag ctagggaccc tcacctactt ctggtggttt
     5161 ggaaatagca caaagcccct catcaccttg gacagcagca tctctttcac attccttgca
     5221 gagggaacca acaccatcac tgtccaggtt gctgctggga atgccctcat ccaagacaca
     5281 aaagagattg cagtacatga atacttccag tcacagctct tatcgttctc tccaaatctg
     5341 gattaccaca atcctgatat ccctgagtgg aggcaagaca ttggcaatgt catcaagcga
     5401 gctctgatta aagtgaccag tgtcccagaa gaccagatcc tcgtggctgt gttcccgggt
     5461 ctccctactt cagcagagct tttcattctc ccacctaaga acttgacaga gaggaggaaa
     5521 ggccacgaag gggatctcga acaaattgta gagacactat ttaatgcgct taatcaaaac
     5581 ttggtacagt ttgagctgaa gccaggtgtc caggtcattg tttatgtcac acagctgaca
     5641 ttagctcccc tggtggactc cagcgctggg catagcagct ctgccatgct tatgctcctc
     5701 tcggtggtat tcgtcggctt ggctgtgttt ctgatctaca agtttaaaag gaaaatcccc
     5761 tggattaaca tctatgccca agtccaacat gacaaggaac aggagatgat cgggtcagtg
     5821 agccaaagtg agaatgcacc caaaatcaca ctcagtgact tcaccgagcc cgaggagctg
     5881 ctggacaaag agctggacac gcgggtcata ggaagcattg ccactattgc aagcagcgag
     5941 agcacaaagg agatccccaa ctgcactagt gttggatccg actacaaaga ccatgacggt
     6001 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     6061 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6121 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6181 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6241 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6301 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6361 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6421 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6481 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6541 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6601 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     6661 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     6721 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     6781 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     6841 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     6901 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     6961 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     7021 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     7081 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     7141 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     7201 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     7261 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     7321 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     7381 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     7441 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7501 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7561 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7621 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7681 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7741 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     7801 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     7861 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7921 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7981 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     8041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8401 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8581 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     8641 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8701 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     8761 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8821 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8881 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8941 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     9001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     9061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     9121 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9241 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     9301 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
    10201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10861 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11161 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11221 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11281 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11341 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    11401 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    11461 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    11521 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    11581 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    11641 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    11701 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    11761 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    11821 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    11881 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    11941 caagctg
//
