LOCUS V044247 11947 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044247 VERSION V044247 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..5973 /label="Sorcs3(NM_025696)" /note="Sorcs3(NM_025696)" /gene="Sorcs3" CDS 5980..6045 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 6089..6300 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 6313..6581 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 6610..6619 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 6640..7302 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 7372..7425 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 7426..8025 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 8026..8614 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8688..8921 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8993..9114 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 9154..9289 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9322..9338) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9346..9362 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9370..9400) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9415..9436 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9724..10312) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10483..11343) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11344..11448) /gene="bla" /label="AmpR promoter" primer_bind 11922..11938 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggcggcagg cactgagcgc 2341 cccgcaggct ggcccggggc acccctcgcc cggactgggc tgctgctctt gtcaacatgg 2401 gtcctggccg gcgccgagat cacttggggc gcgacaggag gtccggggcg cctggtgtcc 2461 ccggcttcac gaccaccagt gttgcctcct ctcttgccac gggcagcgga gaaccggtgg 2521 cctgaggagc tggcatctgc acggagagcc gctgcaccga gacgccggtc caggctagag 2581 ccactatccc aggcgagccg cggggagata cggactgaag ctgcgggaat gtcgcctgaa 2641 ggcgctaggt gggtcccggg cattccatct cctagccagg cgggcagtgc gaggaggact 2701 cgccgggcgc agcccccaag ccccctggag cgcggggact cttgggctac tgctctcgca 2761 gatggtgcca aaggaagccg tcctcacacc aagggttcgc gagaggaggt gagggcgacg 2821 cggactggag gagcgtccac tgaggagctc cggctgccca gcacgtcttt tgcactgacc 2881 ggggactcgg ctcataacca agccatggtg cactggtcgg gacacaacag cagtgtcata 2941 ctgatcctga cgaagctcta tgacttcaac ctggggagtg tgactgagag ttctctgtgg 3001 aggtcagtag attatggcgc cacctatgaa aaacttaatg acaaagtggg tttgaagacc 3061 gtcctcagtt atctctatgt caaccccacc aacaaaagga agatcatgct ccttagtgac 3121 cccgagatgg agagcagtgt actgatcagc tcagatgaag gggcgaccta ccaaaagtac 3181 cggcttacat tctacatcca gagcctgctc ttccatccca agcaggagga ctgggtgctg 3241 gcctacagct tggaccaaaa gctttatagc tccatggact tcggcagacg atggcaactc 3301 atgcatgaac gcatcacgcc caacaggttt tactggtcgg tgtctggact ggataaggag 3361 gcagacctgg tgcacatgga agtgaggacg gcagatggat atgcccacta cctcacctgc 3421 agaatccaag aatgtgctga gacaactcgg agcggccctt ttgcccgctc cattgacatc 3481 agctccttgg ttgtccagga tgaatatatc ttcattcagg taacaatagg tggacgagcc 3541 agctactacg tgtcgtatcg aagagaagcc tttgcccaga taaagctgcc caagtactcc 3601 ttgccaaagg acatgcacat tatcagtacc gatgagaacc aagtgtttgc agccgtccaa 3661 gaatggaacc agaatgacac ctacaacctc tacatctcag acacacgtgg gatctacttt 3721 accctggcta tggagaacat taagagcagc cgaggtctaa tgggaaacat cattattgaa 3781 ttatatgagg tagcaggtat caaggggata ttcctggcaa acaagaaggt ggatgaccag 3841 gtgaagacat acatcactta caacaaaggc agggattggc gtctcctgca agctccagat 3901 gtggacctga gaggaagccc agtgcactgt ttactgccct tctgttccct acacttgcat 3961 ctgcagctct ctgaaaaccc atattcctct ggaagaatct ccagcaagga cacagcgcct 4021 ggacttgtgg ttgccacggg taatattggc tccgaactct cctatacgga tatcggtgtg 4081 ttcatctcct ctgatggggg caacacatgg agacagatct ttgatgaaga atacaatgtc 4141 tggttcctag actggggtgg tgctctggtg gccatgaagc acacacctct gccagtcagg 4201 catttgtggg tgagttttga tgagggccat tcctgggaca agtatggttt cacattgctg 4261 cctcttttcg tggatggggc gttggtggag gcaggagtag agacgcacat catgacagtt 4321 tttggccact tcagcctgcg ctccgaatgg cagttggtga aagtggacta caagtctatc 4381 ttcagccggc gctgtaccaa ggaggacttt gagacttggc acctgctcaa tcagggagag 4441 ccttgtgtca tgggggaaag gaaaatattc aagaaacgca agccaggcgc tcagtgtgcc 4501 cttggcagag agtactctgg gtcggtggtg tcagaaccct gtgtctgtgc ggactgggac 4561 ttcgaatgtg actatggcta tgagagacat ggagagagcc agtgtgtccc agctttctgg 4621 tacaacccag cttccccttc aaaggactgc agccttggtc aaagctacct taacagcact 4681 gggtaccgga ggattgtatc caacaactgc acagatgggc taagggataa gtactctgct 4741 aagactcagt tgtgtcctgg aaaagctcct cggggcctcc atgtggtgac cactgacggg 4801 cgcctggtgg cagaacaggg ccacaatgca accttcatca ttctaatgga ggagggtgac 4861 cttcaaagga caaacatcca gcttgacttt ggagatgggg tggctgtatc ctatgccaac 4921 ttcagcccca ttgaggatgg catcaggcat gtgtataaga gcgcggggat cttccaggtg 4981 acagcttatg cggagaacaa ccttggctcg gacacagcct tcctcttcct gcatgtggtt 5041 tgtccagtgg aacacgttca ccttcgagtc ccattcgtcg caatacggaa taaggacgtc 5101 aacatcagtg cagttgtgtg gcccagtcag ctagggaccc tcacctactt ctggtggttt 5161 ggaaatagca caaagcccct catcaccttg gacagcagca tctctttcac attccttgca 5221 gagggaacca acaccatcac tgtccaggtt gctgctggga atgccctcat ccaagacaca 5281 aaagagattg cagtacatga atacttccag tcacagctct tatcgttctc tccaaatctg 5341 gattaccaca atcctgatat ccctgagtgg aggcaagaca ttggcaatgt catcaagcga 5401 gctctgatta aagtgaccag tgtcccagaa gaccagatcc tcgtggctgt gttcccgggt 5461 ctccctactt cagcagagct tttcattctc ccacctaaga acttgacaga gaggaggaaa 5521 ggccacgaag gggatctcga acaaattgta gagacactat ttaatgcgct taatcaaaac 5581 ttggtacagt ttgagctgaa gccaggtgtc caggtcattg tttatgtcac acagctgaca 5641 ttagctcccc tggtggactc cagcgctggg catagcagct ctgccatgct tatgctcctc 5701 tcggtggtat tcgtcggctt ggctgtgttt ctgatctaca agtttaaaag gaaaatcccc 5761 tggattaaca tctatgccca agtccaacat gacaaggaac aggagatgat cgggtcagtg 5821 agccaaagtg agaatgcacc caaaatcaca ctcagtgact tcaccgagcc cgaggagctg 5881 ctggacaaag agctggacac gcgggtcata ggaagcattg ccactattgc aagcagcgag 5941 agcacaaagg agatccccaa ctgcactagt gttggatccg actacaaaga ccatgacggt 6001 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg 6061 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 6121 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 6181 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 6241 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 6301 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 6361 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 6421 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 6481 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 6541 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 6601 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 6661 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 6721 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 6781 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 6841 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 6901 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 6961 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 7021 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 7081 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 7141 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 7201 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 7261 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 7321 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 7381 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 7441 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 7501 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 7561 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 7621 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 7681 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 7741 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 7801 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 7861 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 7921 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 7981 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 8041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 8101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 8161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 8221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 8281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 8341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 8401 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc 8461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 8521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 8581 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta 8641 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 8701 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca 8761 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8821 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8881 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 8941 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 9001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 9061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9121 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 9181 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 9241 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 9301 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 9361 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 9421 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 9481 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 9541 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 9601 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 9661 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 9721 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 9781 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9841 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 9901 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 9961 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 10021 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 10081 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 10141 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 10201 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 10261 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 10321 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 10381 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 10441 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 10501 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 10561 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 10621 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 10681 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 10741 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 10801 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10861 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10921 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 10981 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 11041 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 11101 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 11161 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 11221 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 11281 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 11341 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 11401 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 11461 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 11521 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 11581 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 11641 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 11701 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 11761 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 11821 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 11881 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 11941 caagctg //