LOCUS       .                       9976 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2011..2214
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2303..3061
                     /label="QuasAr2"
                     /note="achaerhodopsin-3 (Arch) derivative with improved
                     voltage sensitivity (Hochbaum et al., 2014)"
     CDS             3167..3883
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     promoter        4174..4385
                     /label="EF-1α core promoter"
                     /note="core promoter for human elongation factor EF-1α"
     LTR             4398..4666
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4701..5408
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             5472..6068
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    6072..6659
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6733..6966
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7038..7172
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7178..7313
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7399..7429)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7444..7465)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7753..8341)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8515..9372)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(9373..9477)
                     /label="AmpR promoter"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatggtttc tatcgcactg caggctggtt atgacctcct
     2341 cggagatggt agacctgaaa gtctgtggct cggaattggt acactgctga tgctgatcgg
     2401 aaccttctac ttcctggtta gagcgtgggg agagactgac aaggatgcac gggagtacta
     2461 cgcagtgaca atcctggtca gcggtattgc cagtgcagct tatctgtcaa tgttctttgg
     2521 tattggcctc acagaggtgc cagtaggtgg agagatgctg aacatctact acgcaagata
     2581 cgcacagtgg ctgttcacta caccactgtt gctgctgcac ctggcactcc tggccaaagt
     2641 tgatagagtg accatcggca ctctcgttgg cgtcgatgct ctgatgattg tcacaggcct
     2701 gataggaaca ctgtcacata ctgccattgc cagatactct tggtggctgt tctcaactat
     2761 ctgtatgatc gtcgttctgt atgtcctggc cacttctctg cggtcagccg ctaaggagag
     2821 aggcccagag gttgcctcaa ccttcaacat cctcaccgca ctggttctgg tcctgtggac
     2881 tgcttaccct atcatctgga ttatcggcac cgagggtgcc ggagttgtgg gcctgggaat
     2941 cgaaactttg ctgttcatgg tgctggacgt caccgccaag gtgggcttcg gttttatcct
     3001 gctcaggtca agagccattc tgcaagatac agaagcacct gaaccttcag ctggagctga
     3061 taccggtgcc gccgaccgcc cggtagtagc agtgagcaag gcggccgcca agagcaggat
     3121 caccagcgag ggcgagtaca tccccctgga ccagatcgac atcaacgtgg tgagcaaggg
     3181 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3241 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3301 gaagttcatt tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3361 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3421 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3481 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3541 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3601 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     3661 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3721 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3781 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3841 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtggga gcggaggtac
     3901 ccagagccag cctatcctga acaccaagga gatggcccct cagagcaagc ctcctgagga
     3961 gctggagatg agcagcatgc ctagccctgt ggcccctctg cctgccagga ccgagggcgt
     4021 gatcgacatg aggagcatga gcagcatcga cagcttcatc agctgcgcca ccgacttccc
     4081 tgaggccacc aggttctaca agttctgcta cgagaacgag gtgtaaggat ccgcggccgc
     4141 gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     4201 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
     4261 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     4321 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     4381 cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag
     4441 gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa
     4501 ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc
     4561 tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc
     4621 aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg
     4681 cgcctacgct agacgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     4741 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     4801 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4861 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     4921 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     4981 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     5041 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     5101 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     5161 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     5221 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     5281 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     5341 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     5401 tgtacaaggg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     5461 atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5521 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5581 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5641 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5701 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5761 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5821 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5881 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5941 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6001 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6061 ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6121 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6181 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6241 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6301 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6361 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6421 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6481 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6541 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6601 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc
     6661 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6721 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     6781 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6841 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6901 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6961 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7021 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7141 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     7201 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7261 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     7321 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7381 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7441 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7501 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7561 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8161 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8401 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8461 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8641 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8821 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9361 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9421 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9481 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9541 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     9601 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9661 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     9721 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     9781 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     9841 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     9901 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     9961 ggccagtgcc aagctg
//
