LOCUS       V046540                 9926 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046540
VERSION     V046540
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4020
                     /label="Slc43a2(NM_001199283)"
                     /note="Slc43a2(NM_001199283)"
                     /gene="Slc43a2"
     promoter        4085..4296
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4309..4577
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4606..4615
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4636..5298
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5368..5421
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5422..6021
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6022..6609
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6683..6916
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6988..7109
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7128..7263
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7301..7317)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7325..7341
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7349..7379)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7394..7415
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7703..8291)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8462..9322)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9323..9427)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9901..9917
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgcccaccct ggccactgcc
     2341 catcggcgcc gctggtggat ggcctgcacc gctgtgttgg aaaacctcct tttctccgca
     2401 gtcctcctgg gctggggttc gctgctcatc atgctcaagt ccgagggctt ttactcctac
     2461 ctgtgtacga agccagagaa tgtcactaac agcacggtcg ggggcagcgc agagccggaa
     2521 cccgaggagt tgagcctggt gaatggctgg ctcagctgta aggcccagga tgagattctg
     2581 aatttggcct tcaccgtggg ctccttcctg ctcagtgcca tcaccctgcc tctgggcatc
     2641 atcatggaca agtatggtcc aaggaagctc aggctgctgg gcagtgcttg ctttgctgtc
     2701 tcctgcttgc tgattgcata tggagcaagt aacccagact cgctctctgt gctcatcttt
     2761 atcgccttgg ctctgaacgg ctttgggggg atgtgcatga cgttcacttc gttaacactg
     2821 cccaatatgt tcggcgacct tcggtccaca tttattgcct tgatgattgg atcctacgct
     2881 tcctcagcag ttaccttccc aggaataaag ctcatctacg acgctggcgc ctccttcatt
     2941 ggcatcctag tggtctgggc tggctgctct ggcctggttt ttttcaactg tttcttcaac
     3001 tggccactcg agcccttccc aggcccagag gacatggact actcggtgaa gatcaagttc
     3061 agctggctag gctttgacca caagatcaca gggaagcagt tctacaagca ggtgaccaca
     3121 gtggggcgcc gtctgagcgt gggcagctct atgcggactg ccaaggagca agccgccctg
     3181 caggagggcc acaagctgtg tctgtccact gtggacctgg aggtgaagtg ccagcctgat
     3241 gctgcagcgg ccccatcgtt tatgcacagt gtgttcagcc ccctcctggt gctcagcctg
     3301 gtcaccatgt gtgtcacaca gctgcgactt atcttctaca tgggggctat gaacagcatc
     3361 cttgagttcc tggtcagggg ggaccagaag acagttgccc tctatacctc catctttggc
     3421 gcactccagc tgctctgcct gctgacagct cctgtcatcg gctacatcat ggactggaag
     3481 ctgaaagagt gtgaagatac ttcagaggag cctgaggaga aagaaggcac tcaaggtgaa
     3541 aagaagcaga aacgagacag gcagattcag aaagtcacga atgccatgcg ggccttcgcc
     3601 tttacaaacg tgctgcttgt gggttttggg gtgacctgcc tcattcccaa cctgcctcta
     3661 cagatcttct ccttcgtcct gcacacaatt gtgcgaggat tcatccactc tgccgtaggg
     3721 ggcctatacg ctgccgtgta cccctccaca cagtttggta gcctcactgg actgcagtcc
     3781 ctggtcagtg cgctctttgc tctcctgcag cagccgctgt atctggccat gatgggtcct
     3841 ctgggaggag accctctgtg ggtgaacgtg ggtctgctcg ccatgagcat gctgggcttc
     3901 tgcctgcccc tttacctcat ctgctaccgg cgccagctgg agaggcagct gcagcagaag
     3961 agggaagaca gcaagctgtt ccttaagatc aatggctcat ccaaccggga ggctttcgtg
     4021 tagggatccc tgcgatatcc agcgcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4081 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4141 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4201 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4261 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     4321 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     4381 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4441 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4501 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4561 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     4621 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     4681 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     4741 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     4801 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     4861 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     4921 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     4981 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5041 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5101 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     5161 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     5221 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     5281 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     5341 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     5401 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     5461 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5521 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5581 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5641 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5701 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5761 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5821 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5881 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5941 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6001 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6061 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6121 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6181 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6241 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6301 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6361 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6421 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6481 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6541 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg
     6601 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6661 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     6721 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6781 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6841 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6901 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6961 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7021 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7081 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     7141 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7201 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7261 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     7321 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7381 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7441 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7501 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7561 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7621 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7681 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7741 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7801 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7861 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7921 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7981 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8041 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8101 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8161 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8221 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8281 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8341 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8401 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8461 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8521 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8581 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8641 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8701 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8761 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8821 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8881 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8941 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9001 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9061 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9121 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9181 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9241 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9301 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9361 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9421 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9481 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9541 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9601 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9661 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     9721 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     9781 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     9841 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     9901 gtaaaacgac ggccagtgcc aagctg
//
