LOCUS       V041514                 9658 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041514
VERSION     V041514
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4512
                     /codon_start=1
                     /gene=""
                     /note="homolog of budding yeast Sir2"
                     /product="mouse NAD+-dependent protein deacetylase
                     sirtuin-1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MADEVALALQAAGSPSAAAAMEAASQPADEPLRKRPRRDGPGLGR
                     SPGEPSAAVAPAAAGCEAASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRR
                     EPRAADDFDDDEGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHA
                     SSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSE
                     PPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPD
                     LPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNID
                     TLEQVAGIQRILQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIM
                     KPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINR
                     EPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRPQKELVHLS
                     ELPPTPLHISEDSSSPERTVPQDSSVIATLVDQATNNNVNDLEVSESSCVEEKPQEVQT
                     SRNVENINVENPDFKAVGSSTADKNERTSVAETVRKCWPNRLAKEQISKRLEGNQYLFV
                     PPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPLEDESEIEEFYNGLED
                     DTERPECAGGSGFGADGGDQEVVNEAIATRQELTDVNYPSDKS"
                     /label="SIRT1"
     CDS             4519..4584
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4628..4839
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4852..5120
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5147..5746
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5753..6341
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6415..6648
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6720..6841
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6860..6995
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7033..7049)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7057..7073
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7081..7111)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7126..7147
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7435..8023)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8194..9054)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9055..9159)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9633..9649
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggac gaggtggcgc tcgcccttca ggccgccggc
     2341 tccccttccg cggcggccgc catggaggcc gcgtcgcagc cggcggacga gccgctccgc
     2401 aagaggcccc gccgagacgg gcctggcctc gggcgcagcc cgggcgagcc gagcgcagca
     2461 gtggcgccgg cggccgcggg gtgtgaggcg gcgagcgccg cggccccggc ggcgctgtgg
     2521 cgggaggcgg caggggcggc ggcgagcgcg gagcgggagg ccccggcgac ggccgtggcc
     2581 ggggacggag acaatgggtc cggcctgcgg cgggagccga gggcggctga cgacttcgac
     2641 gacgacgagg gcgaggagga ggacgaggcg gcggcggcag cggcggcggc agcgatcggc
     2701 taccgagaca acctcctgtt gaccgatgga ctcctcacta atggctttca ttcctgtgaa
     2761 agtgatgacg atgacagaac gtcacacgcc agctctagtg actggactcc gcggccgcgg
     2821 ataggtccat atacttttgt tcagcaacat ctcatgattg gcaccgatcc tcgaacaatt
     2881 cttaaagatt tattaccaga aacaattcct ccacctgagc tggatgatat gacgctgtgg
     2941 cagattgtta ttaatatcct ttcagaacca ccaaagcgga aaaaaagaaa agatatcaat
     3001 acaattgaag atgctgtgaa gttactgcag gagtgtaaaa agataatagt tctgactgga
     3061 gctggggttt ctgtctcctg tgggattcct gacttcagat caagagacgg tatctatgct
     3121 cgccttgcgg tggacttccc agacctccca gaccctcaag ccatgtttga tattgagtat
     3181 tttagaaaag acccaagacc attcttcaag tttgcaaagg aaatatatcc cggacagttc
     3241 cagccgtctc tgtgtcacaa attcatagct ttgtcagata aggaaggaaa actacttcga
     3301 aattatactc aaaatataga taccttggag caggttgcag gaatccaaag gatccttcag
     3361 tgtcatggtt cctttgcaac agcatcttgc ctgatttgta aatacaaagt tgattgtgaa
     3421 gctgttcgtg gagacatttt taatcaggta gttcctcggt gccctaggtg cccagctgat
     3481 gagccacttg ccatcatgaa gccagagatt gtcttctttg gtgaaaactt accagaacag
     3541 tttcatagag ccatgaagta tgacaaagat gaagttgacc tcctcattgt tattggatct
     3601 tctctgaaag tgagaccagt agcactaatt ccaagttcta taccccatga agtgcctcaa
     3661 atattaataa atagggaacc tttgcctcat ctacattttg atgtagagct ccttggagac
     3721 tgcgatgtta taattaatga gttgtgtcat aggctaggtg gtgaatatgc caaactttgt
     3781 tgtaaccctg taaagctttc agaaattact gaaaaacctc cacgcccaca aaaggaattg
     3841 gttcatttat cagagttgcc accaacacct cttcatattt cggaagactc aagttcacct
     3901 gaaagaactg taccacaaga ctcttctgtg attgctacac ttgtagacca agcaacaaac
     3961 aacaatgtta atgatttaga agtatctgaa tcaagttgtg tggaagaaaa accacaagaa
     4021 gtacagacta gtaggaatgt tgagaacatt aatgtggaaa atccagattt taaggctgtt
     4081 ggttccagta ctgcagacaa aaatgaaaga acttcagttg cagaaacagt gagaaaatgc
     4141 tggcctaata gacttgcaaa ggagcagatt agtaagcggc ttgagggtaa tcaatacctg
     4201 tttgtaccac caaatcgtta catattccac ggtgctgagg tatactcaga ctctgaagat
     4261 gacgtcttgt cctctagttc ctgtggcagt aacagtgaca gtggcacatg ccagagtcca
     4321 agtttagaag aacccttgga agatgaaagt gaaattgaag aattctacaa tggcttggaa
     4381 gatgatacgg agaggcccga atgtgctgga ggatctggat ttggagctga tggaggggat
     4441 caagaggttg ttaatgaagc tatagctaca agacaggaat tgacagatgt aaactatcca
     4501 tcagacaaat caggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     4561 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     4621 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4681 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4741 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4801 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     4861 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     4921 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     4981 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5041 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5101 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa
     5161 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     5221 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     5281 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5341 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5401 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5461 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5521 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5581 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5641 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5701 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc
     5761 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5821 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5881 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5941 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6001 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6061 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6121 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     6181 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6241 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6301 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     6361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6421 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt
     6481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6661 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     6721 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6781 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6841 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc
     6901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     6961 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa
     7021 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7321 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7381 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7441 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7501 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7561 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7621 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7681 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7741 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7801 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7861 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7921 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7981 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8041 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8101 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8161 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8221 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8281 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8341 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8401 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8461 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8521 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8581 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8641 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8701 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8761 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8821 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8881 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8941 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9001 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9061 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9121 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9181 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     9241 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     9301 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     9361 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     9421 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     9481 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     9541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     9601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
