LOCUS       V033006                10334 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033006
VERSION     V033006
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4380
                     /label="Siglecg(NM_172900)"
                     /note="Siglecg(NM_172900)"
                     /gene="Siglecg"
     CDS             4399..4428
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        4493..4704
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4717..4985
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5014..5023
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5044..5706
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5776..5829
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5830..6429
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6430..7017
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7091..7324
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7396..7517
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7536..7671
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7709..7725)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7733..7749
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7757..7787)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7802..7823
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8111..8699)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8870..9730)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9731..9835)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10309..10325
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cactgctgct gttcctgctg
     2341 tccttcctgt tggatgggcc tcaaggtcag atggagagct acttcctaca ggtgcagaga
     2401 attgtgaagg cacaggaggg tctgtgcatc ttcgtgccct gctccttctc ctcccctgag
     2461 ggaaaatggc ttaaccgttc cccactttat ggctactggt tcaaaggcat cagaaaacca
     2521 tcactttcat ttccagtggc cacaaataac aaagataaag tgttagaatg ggaagcccga
     2581 gggcgcttcc agctcttggg ggatatctct aaaaagaact gttccttgct aatcaaagat
     2641 gttcagtggg gagactcaac aaactatttc ttccggatgg agagaggatt tgagagattc
     2701 agtttcaagg aggagttcag gctacaagtg gaagccctga ctcagaagcc agatatcttc
     2761 attcctgagg tcctggagcc tggggagcca gtgaccgttg tctgcttgtt ttcctggacc
     2821 ttcaaccaat gcccagctcc ttctttctcc tggatggggg atgctgtctc cttccaagaa
     2881 agcagaccgc acacatccaa ttactcagtt ctgagcttta tcccaggact tcaacaccat
     2941 gatactgagc tcacatgtca gctggacttc tctagaatga gcacacaaag gactgtccga
     3001 ctaagagtgg cctatgcccc cagatctctt gctatcagca ttttccatga caatgtatca
     3061 gtcccagact tgcatgagaa tccttcacat ctggaagttc agcaaggcca gtctcttcgc
     3121 ctcctctgta ctgctgacag ccagccccct gctacactga gctgggtcct agaggaccaa
     3181 gtcctttctt ggtctagccc tgtggggtcg agaaccctgg cactagagct gccctgggtg
     3241 aaagctgggg actctggaca ctacacttgc caagcagaga ataggctggg ctcccagcaa
     3301 cataccctgg acctctctgt gctgtacccc ccacaggacc tgagagtgac tgtttcccaa
     3361 gcaaacagga cagtgttgga aatcctcagg aatgccatct ccctcccagt cctggagggc
     3421 caaagcctgt gcctagtctg tgtcacctat agcaatcccc cagccaatgt gagttgggct
     3481 tgggtgacac agaccctgat cccaatccag tcttcagagc ctggggtcct ggagctgcct
     3541 ctggtccaga gagaacatga aggagaattc acctgtgctg cacagaaccc actgggtgcc
     3601 cagcgcatct ctctgagcct ctctgtgcac tacccgcccc agatgtccag cccctcctgc
     3661 tcctgggagg ccaagggtct gcactgcaac tgctcctcca gagcctggcc tgccccctcc
     3721 ctgcgctggc ggctggggga ggggctgctg gaggggaaca gcagcaatgc ctccttcaca
     3781 gtcactttca gctcacttgg accctgggtc aacagctccc tgagcctcct tcaggagctg
     3841 gggcccagcc tctggctcag ctgtgagtcc tggaacaccc atggagccca gaccacctct
     3901 gtcctgctgc tacctgataa agacagtgcc acagcattct ccaagggagc agttttgggc
     3961 tttggcatca cagccctcct tgccctgtgc ctcatcgtgg tcatcgtgaa gaccctccag
     4021 aagaaaggaa cccaggagga accctccagg cccaagctct cccggggcag cacgatcctg
     4081 gactacatca atgtggtccc caagaccagg tccctggctc ggaattggaa agctgaacca
     4141 gatgcccctt ctaggagctc acccctggac actcacttcc ctaaaccaaa gaagaagcaa
     4201 aaggacccac atttcacgta ccctggttgc ccagatccca catcatcctc tcaggtccca
     4261 gtctctgaga ataacccgga agaactccac tatgctgctc tcaacttctc ccgcctgaga
     4321 ctgcaggaga cccaggatcc ccaagacacc tattctgatt acacagaggt cagagtccac
     4381 tcggtaccaa gctttctaga acaaaaactc atctcagaag aggatctgtg aggatccctg
     4441 cgatatccag cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4681 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4741 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     4801 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     4861 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     4921 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     4981 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5041 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5101 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5161 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5221 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     5281 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     5341 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     5401 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     5461 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     5521 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     5581 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     5641 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     5701 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     5761 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     5821 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5881 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5941 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6001 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6061 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6121 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6181 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6241 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6301 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6361 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6421 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6481 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6541 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6601 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6661 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6721 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6781 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6841 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6901 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6961 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     7021 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7081 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7141 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7201 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7261 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7321 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7381 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7441 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7501 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7561 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7621 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7681 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8521 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9841 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9901 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     9961 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10021 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10081 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10141 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10201 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10261 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10321 ccagtgccaa gctg
//
