LOCUS       V040441                12485 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040441
VERSION     V040441
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..7404
                     /label="Siglec1(NM_011426)"
                     /note="Siglec1(NM_011426)"
                     /gene="Siglec1"
     promoter        7455..7666
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7679..7947
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7974..8573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8580..9168
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9242..9475
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9547..9668
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9687..9822
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9860..9876)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9884..9900
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9908..9938)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9953..9974
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10262..10850)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11021..11881)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11882..11986)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12460..12476
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcacctg ggcactggga tgtgtgtcct gttctccctg
     2341 ctcctgctgg cctctgtctt ctcactaggc cagaccacat ggggtgtctc cagtcccaag
     2401 aatgtgcagg gcttgtcggg atcctgcctg ctcattccct gcatcttcag ctaccctgcc
     2461 gatgtcccag tgtccaatgg catcacagcc atctggtact atgactactc gggcaagcgg
     2521 caggtggtaa tccactcagg ggaccccaag ctggtggaca agcgtttcag gggtcgagct
     2581 gaactgatgg ggaacatgga ccacaaggtg tgcaacctgt tgctcaaaga cttgaagcct
     2641 gaagactctg gcacctacaa cttccgcttt gagatcagtg atagcaaccg ctggttagat
     2701 gtcaaaggca ccacggtcac tgtgacaacg gatcccagcc cccccactat taccattcct
     2761 gaggagctgc gtgaaggcat ggagaggaac ttcaactgtt ccacacccta cctgtgcctg
     2821 caggagaagc aagtcagcct gcagtggcga ggccaggacc ccacccactc tgtcacctcc
     2881 agcttccaga gcctcgagcc cactggcgtc tatcaccaga cgaccctaca tatggcccta
     2941 tcctggcagg accacggtcg gaccctgctc tgccagttct cattgggcgc acacagtagt
     3001 cggaaagagg tttacctgca agtgccacat gcccccaaag gtgtggagat cctcctcagc
     3061 tcctcaggga ggaacatcct tcccggggat ccagtcacac tcacctgcag agtgaacagc
     3121 agctatcctg ctgtcagtgc cgtgcagtgg gccagggacg gagtgaacct cggagtcacg
     3181 ggacatgtgc ttcggctgtt ctcagcagcc tggaatgatt ctggggccta cacctgccaa
     3241 gcaacaaatg atatgggctc tctggtgtca tccccgctca gcctccatgt ttttatggct
     3301 gaagtcaaaa tgaaccccgc agggcccgtc ttggaaaatg agacagtgac tctgctctgt
     3361 agcacgccga aggaggctcc ccaggagctc cgctatagct ggtacaagaa ccacattctc
     3421 ctggaagatg cccatgcctc aaccttgcac ctgcctgcag tcaccagggc tgatactggc
     3481 ttctacttct gtgaagtgca gaatgcccag ggcagtgagc gctccagtcc attgagtgtg
     3541 gtggtcagat atccacccct tactccagac ctgaccacct tcctggagac acaggccgga
     3601 cttgtgggca tcttgcattg ctccgtggtc agtgagcccc tggctactgt ggtgctgtca
     3661 cacggaggcc tcacgttggc ctccaactct ggagaaaatg acttcaaccc ccgattcagg
     3721 atctcctctg cccccaactc cctgcgccta gaaatccgag acttgcagcc agcagacagc
     3781 ggagagtaca catgcttagc tgtcaactcc cttggaaact caacgtccag cctagacttc
     3841 tatgctaatg tggcccgact cctcatcaac ccttcagcag aggttgtgga agggcaggcg
     3901 gtgaccctga gctgcaggag tggcctgagc ccagctcctg acactcgctt ctcctggtac
     3961 ctgaacggag ctctacttct ggaaggatcc agcagcagcc tcctgcttcc tgcggcttcc
     4021 agcactgatg cgggctcata ctactgtagg acgcaggctg gccccaacac cagcggcccc
     4081 tccctgccta ctgtcctcac tgtgttctat cccccaagaa agcccacatt cactgccagg
     4141 ctggatttgg atacctctgg agtcggggat ggacgacggg gcatcctctt gtgccacgta
     4201 gacagcgatc ccccagccca gctacggctt ctccacaaag gccatgttgt ggccacttct
     4261 ctgccatcaa ggtgtgggag ctgttcccag cgcacaaaag tcagcagaac ctccaactca
     4321 ctgcacgtgg agatccagaa gcctgtatta gaggatgagg gcgtgtacct gtgtgaggct
     4381 agcaacacat tgggcaactc ctcagccgca gcctctttca atgctaaggc cactgtactg
     4441 gtcatcacac cgtcaaatac actgcgtgaa ggcacagagg ccaacctaac ttgcaacgtg
     4501 aaccaggagg ttgctgtcag ccctgccaac ttctcctggt tccggaatgg agtgctgtgg
     4561 acccagggat cactggagac tgtgaggctg cagcctgtgg ccagaactga tgctgctgtc
     4621 tatgcctgcc gcctcctcac cgaggatggg gctcagctct cggctcctgt ggtcctaagt
     4681 gtgctgtatg ccccagaccc tccaaagctg tcagccctcc tagatgtggg tcagggccac
     4741 atggccgtgt tcatctgcac tgtggacagc tatcccctgg ctcacctgtc tctgttccgt
     4801 ggggaccatc tcctggccac caacttggaa ccccagcgtc cctcccatgg caggatccag
     4861 gccaaggcca cagccaactc cctgcagcta gaggtccgag aactaggtct tgtggactct
     4921 ggaaactacc actgtgaagc caccaatatt cttgggtcag ccaacagttc actcttcttc
     4981 caggtcagag gagcctgggt ccaggtttca ccatcacctg agctccggga gggccaggct
     5041 gtggtcctga gctgccaggt gcccacagga gtctctgagg ggacctcata cagctggtat
     5101 caggatggcc gccccctcca ggagtcaacc tcatctacac tccgcattgc agccataagt
     5161 ctgaggcaag ctggtgccta ccattgccaa gctcaggccc cagacacagc tattgccagc
     5221 ctggctgccc ctgtcagcct ccatgtgtcc tataccccac gtcatgttac actcagtgcc
     5281 ctgctgagca cggaccctga gcgactaggc cacctggtgt gcagtgtaca aagtgaccct
     5341 ccagcgcagc tgcaactgtt tcaccggaat cgcctcgtgg cctctaccct acaaggcgcg
     5401 gacgaattgg caggcagtaa tccccggctg catgtgactg tgctccccaa tgagctgcgc
     5461 ctgcagatcc actttccaga gctggaggat gacgggacct atacatgcga agccagcaac
     5521 acactgggcc aggcctcggc tgcagctgac ttcgatgccc aggctgtgcg agtgactgtg
     5581 tggcccaatg ccactgtgca agaggggcag caggtgaacc tgacctgctt ggtgtggagc
     5641 acccaccagg actcactcag ctacacatgg tacaagggcg ggcaacaact ccttggtgcc
     5701 agatccatca ccctgcccag tgttaaggtt ttggatgcta cctcctaccg ctgtggtgtg
     5761 gggctccccg gccacgcacc ccatctctcc agacccgtga ccctggatgt cctccatgct
     5821 ccccgaaacc tgcggctgac ctacctccta gagacccagg gcaggcagct ggccctggta
     5881 ctgtgtacgg tggatagtcg tccacctgcc cagctaactc tcagccatgg tgaccagctt
     5941 gtagcctcct caactgaagc ctctgtcccc aacaccctgc gcctagagct tcaggatcca
     6001 aggcctagta atgaggggct ctatagctgc tctgcccaca gcccattggg caaggccaac
     6061 acgtccctgg aacttctgct ggaaggtgtc cgagtgaaaa tgaatccctc tggtagtgta
     6121 cccgagggag agcctgtcac agtgacctgc gaggaccctg ctgccctctc atccgccctc
     6181 tatgcctggt ttcacaatgg ccattggctt caggagggac cggcttcctc actccagttc
     6241 ctggtgacta cacgggctca cgctggtgct tacttttgcc aggtgcatga tacacaaggc
     6301 acacggagct ccagacctgc cagcctgcaa attctctatg ccccccggga tgctgtcctg
     6361 tcttcctttc gagactcaag gaccaggctc atggtcgtga ttcagtgcac cgtggacagt
     6421 gagccacctg ctgagatggt cctatcccac aatggcaagg tgctagctgc cagccacgag
     6481 cgtcacagct cagcatcagg gataggccac atccaggtag cccgaaatgc tcttcgacta
     6541 caagtgcaag atgtgactct gggtgatggc aacacctatg tttgcacagc ccagaataca
     6601 ctgggctcca tcagtaccac ccagaggctt ctgacggaga ctgatatacg tgtgacagct
     6661 gagccaggct tggatgtgcc agagggcaca gctctgaact taagctgcct cctccctggt
     6721 ggctctgggc ccacgggcaa ctcttccttc acgtggttct ggaatcgcca ccgactacat
     6781 tcagctcctg tgcccacact ctccttcacc cctgtggtcc gggctcaggc tgggctgtac
     6841 cactgcaggg ctgatctccc caccggggcc actacctctg ctccagttat gctccgtgtc
     6901 ctctatcccc ccaagacgcc cactctcata gtgtttgtgg agcctcaggg tggccaccag
     6961 ggcatcctcg actgtcgagt ggacagtgag cccctggcca tcctcactct tcaccggggc
     7021 agtcaactag tagcctccaa ccaacttcac gatgctccca ccaagcccca catccgagtc
     7081 actgctcctc ccaatgcctt gagagtggac atagaggagc tcggccctag caatcaaggg
     7141 gagtatgtgt gcactgcctc caacactctg ggctctgcct cagcctctgc ctactttggg
     7201 accagagctc tgcaccaact gcagctgttc cagaggctgc tctgggtcct gggatttctg
     7261 gcaggcttcc tgtgcctgct gctgggtctg gtggcctatc acacctggag aaagaagagt
     7321 tctaccaagc tgaatgagga tgagaattca gcagagatgg ccactaagaa aaatactatc
     7381 caggaggaag tggttgctgc tctctgagga tccgcggccg cgaaggatct gcgatcgctc
     7441 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     7501 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     7561 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     7621 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
     7681 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
     7741 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
     7801 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
     7861 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
     7921 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg
     7981 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
     8041 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
     8101 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     8161 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     8221 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     8281 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     8341 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     8401 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     8461 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     8521 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca
     8581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     9001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     9181 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9241 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct
     9301 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9361 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9421 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     9481 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     9541 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9601 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9661 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
     9721 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9781 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg
     9841 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9901 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9961 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
    10021 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    10081 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    10741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    11041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    11101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    11161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    11821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    11881 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11941 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    12001 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    12061 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
    12121 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    12181 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    12241 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    12301 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    12361 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    12421 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
    12481 agctg
//
