LOCUS       V029380                11176 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029380
VERSION     V029380
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5202
                     /label="Sh3bp4(NM_133816)"
                     /note="Sh3bp4(NM_133816)"
                     /gene="Sh3bp4"
     CDS             5209..5274
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5318..5529
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5542..5810
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5839..5848
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5869..6531
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6601..6654
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6655..7254
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7255..7843
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7917..8150
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8222..8343
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8383..8518
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8551..8567)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8575..8591
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8599..8629)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8644..8665
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8953..9541)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9712..10572)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10573..10677)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11151..11167
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagcccagcg tatccgggca
     2341 gccaacgcca gtggcctccc tcggtgcaag tcggagggga ctctgataga cctgagcgag
     2401 ggcttctcag agacaagctt taatgatgtc aaagtgcctt cccccagtgc cttgctcgta
     2461 gacaacccca cgccttttgg aaacgcaaag gaagtgattg ccatcaaaga ctattgccca
     2521 aacaacttca ccaccctgaa gttttccaag ggcgaccacc tctacgtctt ggacacgtct
     2581 ggtggggagt ggtggtacgc tcacaacacc accgagatgg gctacatccc ttcctcctac
     2641 gtgcagccat taaactaccg gaattctacc ctgagtgaca gtggcatgat cgacaatctt
     2701 ccggacagcc ctgaagaagt tgccaaggag ttagatctgc tagggggtgg ctggaccgat
     2761 gaccagaaag aatctggcag gccctatagt aacaaccctt tctggaatgg agtccgaacg
     2821 aacccctttc tgaatgggaa tgcacagccc agtacggatg agctaaaccc caagagtacc
     2881 gtggatttgc tcctgtttga cacgggcaca tcttccttca ctgagtccag ctcggcgacc
     2941 accaacagca ccggcaacat ctttgatgag ctcccagcca ccaatggact ccaggtggag
     3001 cagcctgtca agcgggacaa cccctttttc agaagcaagc gttcctacag cctttcggaa
     3061 ctctccgtcc tgcaagccaa atcggatgct cccccaacgt ccagcttctt cacaggcttg
     3121 aagtcgcctg tcccggaaca gtttcaaagc cgagaagact tccgaaccgc ctggctcaat
     3181 caccggaagc tggcccgttc ctgccatgac ttggatctgc tcggccaaag cccaggctgg
     3241 ggccagaccc aagcagtgga gacaaatatt gtgtgcaagc tggatagctc tgggggctcc
     3301 gtgcagcttc ctgacaccaa catcagtatc catgtgccag agggccatgt agctcctggg
     3361 gagacacagc agatttccat gaaagccctg ctggaccctc cgctggacct taacagtgac
     3421 agatccacca gcgtaagccc tgtggtggag gtgaagctga gtaacctaga ggtgagtacc
     3481 ttcatcatct tggagatgaa ggtctccgcc gaggtgaagg gcgacatctt tagcaaaagc
     3541 acggtggtcc tgcagtgcct gagaagtgac tcaaaggagg ggccttatgt gcccatccct
     3601 ctcgcctaca gctatgggga caccatacag gtacagctgg acaacctgga accctgcatg
     3661 tacctagcca tcgttgccca gggccccaac atcctctacc cttccaccgt gtgggatttc
     3721 atcaataaga gggtcaccgt gggtctctat ggtcccaaac acatccatcc atccttcaag
     3781 acagtagtga ccatttttgg acatgattgt gcccccaaga ccctcctggt gagtgaggtg
     3841 actcggcagg ctcccagtcc tgcccccgtg gccctccagc tgtggggcaa acatcagttc
     3901 attctgtcca ggccccagga tctcagagtg tgtatgttct ccaatatgac taactatgag
     3961 gtcaaggcca acgagcaagc ccgagtagtg agagggtttc agatgaagct gggcaaggtg
     4021 agccgtctga tcttctccgt catctcccag aaccccaatg agctgtccga cttcaccctg
     4081 agggtccagg tcaaggatga tcaggacacg atcctcacgc agttttgtgt ccagactccg
     4141 cagccgcccc ctaaaagcgc cattaaaccg tcgggacaga ggaggtttct taagaagaac
     4201 gaggtcggga agatcatctt gtccccgttt gtcgttacaa ccaagtatcc aacgtttcag
     4261 gaccgccccg tgtctagcct caagtttggc aagcttctaa agacagtggt gcggcagaac
     4321 aagagtcact acctgctgga gtacaagaaa ggtgacgtcg tggctctgct cagcgaggag
     4381 cgcatccggc tcaagggaca gctgtggacc aaggagtggt acatcggcta ctaccagggc
     4441 aaggtgggcc tggtgcacac caagaacgtg ctggtcgtcg gcaaggcccg gcccagcctg
     4501 ttctcggggc ccgagctgag cacctcagtg ctgctggagc agatcctgcg gccctgcaag
     4561 ttccttacct acatctatgc ctctgtgcgg accttactca tggagaacat cagcagctgg
     4621 cgggcctttg ctgatgccct gggttacggc aacctgccgc tcaccttctt ctgccgggca
     4681 gagctggaca gcgagccgga acgggtggct tccgttctgg aaaagctcaa ggaagactgt
     4741 aacaaccctg acaacaagga ccggaagtcc ttccagaagg agctggtgat ggctttgctg
     4801 aaaatggact gccagggcct ggtggtgaga ctcatccagg actttgtgct cctgaccaca
     4861 gctgtcgaag tggcccagcg ctggagggaa ctggctgaga agctagccaa ggtctccaag
     4921 caacagatgg atgcctatga gtctcctcac cgagacagga atggggtagt agacagtgag
     4981 gccatgtgga aacctgccta tgacttctta ctcacctgga gccaccagat tggggacagc
     5041 taccgagatg tcatccagga gttgcacata ggcctggaca agatgaagaa ccccatcacc
     5101 aggcgctgga agcacctcac agggacactg atcctggtga actcactgga catcctgagg
     5161 gcagctgcct tcagtcccgc ggaccatgat gactttgtga tcggatccga ctacaaagac
     5221 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     5281 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5341 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     5401 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5461 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5521 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     5581 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     5641 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     5701 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     5761 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     5821 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     5881 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     5941 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6001 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6061 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6121 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     6181 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     6241 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     6301 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     6361 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     6421 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     6481 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     6541 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     6601 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     6661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6961 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     7201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     7261 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7321 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7381 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7441 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7501 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7561 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7621 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7681 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7741 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7801 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     7861 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7921 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7981 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8041 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8101 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8161 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8221 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8341 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8461 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8521 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     8581 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8641 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8701 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10681 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10741 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10801 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10861 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10921 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10981 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    11041 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    11101 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    11161 ggccagtgcc aagctg
//
