LOCUS       V041678                 9461 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041678
VERSION     V041678
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4293
                     /label="Sdha(NM_023281)"
                     /note="Sdha(NM_023281)"
                     /gene="Sdha"
     CDS             4300..4326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4330..4356
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4360..4386
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4431..4642
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4655..4923
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4950..5549
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5556..6144
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6218..6451
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6523..6644
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6663..6798
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6836..6852)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6860..6876
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6884..6914)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6929..6950
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7238..7826)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7997..8857)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8858..8962)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9436..9452
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccggg gttggcgcag tttcgaggct tcttcgcggg
     2341 cggcgcttgg cgttaactgg ggcgtggcca ggaacactcc aaaaacagac ctgcggcttt
     2401 cacttctctg ttggtgagaa caagaaggca tcagctaaag tttcagatgc aatttctact
     2461 caatacccag tggtggatca tgaatttgat gctgtggttg taggcgctgg aggggcaggc
     2521 ttgcgagctg catttggcct ttctgaggca gggtttaata ctgcatgcct tacaaagctc
     2581 tttcctaccc gatcacatac tgttgcagca cagggaggta tcaatgctgc tctggggaac
     2641 atggaagagg acaactggag atggcatttc tatgacactg tgaaaggctc cgactggctg
     2701 ggggatcagg atgccatcca ttacatgaca gagcaagctc ctgcctctgt ggttgagcta
     2761 gaaaattatg gtatgccatt tagcagaact gaagatggga agatttatca gcgtgcattt
     2821 ggtggacaga gcctcaagtt tgggaaaggc gggcaggctc atcggtgttg ctgtgtggct
     2881 gatcggacag gccactcact cttacacacg ttgtatggaa gatctctgcg atatgacacc
     2941 agttattttg tggaatattt tgcactggat cttctgatgg aaaatgggga gtgccgtggt
     3001 gtcattgcac tgtgcataga agatgggtcc atacaccgaa taagagcaaa aaacactgtt
     3061 attgctactg ggggctacgg gcgaacctac ttcagctgta catctgccca taccagcaca
     3121 ggggacggca cagccatggt cactagggct ggtttgcctt gccaggactt agaatttgtt
     3181 cagttccacc ccacaggtat atacggtgct ggctgcctca tcacagaagg gtgtcgtgga
     3241 gaggggggga ttctcatcaa cagtcaaggt gaaaggttca tggagagata cgcacctgtt
     3301 gccaaggacc tggcatcaag agatgttgtg tctcgatcca tgactcttga gatccgtgaa
     3361 ggaagaggct gtggccctga gaaagatcac gtctacctgc agttgcatca tctgcctcct
     3421 gagcaactgg ccacacgtct acccggaatt tcagagacgg ccatgatctt cgctggtgtg
     3481 gatgtcacta aggagcccat cccagtcctc cccactgtgc attacaacat gggtgggatt
     3541 cccactaact acaagggaca ggtgctgaag catgtgaatg gccaggatca gattgtgcct
     3601 ggtctgtatg cctgtgggga ggctgcctgc gcctcagtgc atggtgccaa ccggcttgga
     3661 gcaaattctc tcttggacct tgtagtcttt ggcagagcct gtgccctgag cattgcagaa
     3721 tcttgcaggc ctggagataa agttccttca attaaggcaa atgctggaga agaatcggtt
     3781 atgaatcttg acaagttgag atttgccgat ggaagtataa gaacatcaga actacgccta
     3841 aacatgcaga agtcgatgca gaaccatgct gcagtgttcc gtgtggggag tgtattgcaa
     3901 gaaggctgtg aaaaaatcag tcagctctat ggagacctaa agcatctaaa gacattcgac
     3961 aggggaatgg tttggaacac agacctggtg gagaccctgg agctgcagaa tctgatgctg
     4021 tgcgcactgc agaccatata tggtgcagaa gctcggaagg agtcccgggg agcccatgcc
     4081 agggaagatt acaaagtgcg ggtcgatgag tatgattact ccaagcccat ccagggacag
     4141 cagaagaagc cgtttgggga acactggagg aagcacaccc tctcatatgt ggacatcaag
     4201 actggcaagg ttactttgga gtacagacct gttatcgaca agaccttgaa tgaggctgac
     4261 tgtgccaccg tacctcctgc tatccgctcc tacggatcct acccatacga cgtacctgat
     4321 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     4381 tacgcatagg cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     4441 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     4501 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4561 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4621 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     4681 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     4741 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     4801 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     4861 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     4921 atccaagctg tgaccggcgc ctacgctaga tgaccgagta caagcccacg gtgcgcctcg
     4981 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     5041 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     5101 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     5161 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     5221 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     5281 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     5341 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     5401 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     5461 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     5521 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     5581 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5641 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5701 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5761 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5821 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5881 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5941 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6001 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6061 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6121 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     6181 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6241 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6421 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     6481 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     6541 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6601 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     6661 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6721 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     6781 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     6841 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6901 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6961 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9001 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     9061 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9121 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     9181 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     9241 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     9301 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     9361 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     9421 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
