LOCUS V039172 9298 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039172 VERSION V039172 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4152 /label="SYVN1(NM_172230)" /note="SYVN1(NM_172230)" /gene="SYVN1" CDS 4159..4224 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4268..4479 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4492..4760 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4787..5386 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5393..5981 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6055..6288 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6360..6481 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6500..6635 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6673..6689) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6697..6713 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6721..6751) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6766..6787 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7075..7663) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7834..8694) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8695..8799) /gene="bla" /label="AmpR promoter" primer_bind 9273..9289 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgttccgc acggcagtga tgatggcggc cagcctggcg 2341 ctgaccgggg ctgtggtggc tcacgcctac tacctcaaac accagttcta ccccactgtg 2401 gtgtacctga ccaagtccag ccccagcatg gcagtcctgt acatccaggc ctttgtcctt 2461 gtcttccttc tgggcaaggt gatgggcaag gtgttctttg ggcaactgag ggcagcagag 2521 atggagcacc ttctggaacg ttcctggtac gccgtcacag agacttgtct ggccttcacc 2581 gtttttcggg atgacttcag cccccgcttt gttgcactct tcactcttct tctcttcctc 2641 aaatgtttcc actggctggc tgaggaccgt gtggacttta tggaacgcag ccccaacatc 2701 tcctggctct ttcactgccg cattgtctct cttatgttcc tcctgggcat cctggacttc 2761 ctcttcgtca gccacgccta tcacagcatc ctgacccgtg gggcctctgt gcagctggtg 2821 tttggctttg agtatgccat cctgatgacg atggtgctca ccatcttcat caagtatgtg 2881 ctgcactccg tggacctcca gagtgagaac ccctgggaca acaaggctgt gtacatgctc 2941 tacacagagc tgtttacagg cttcatcaag gttctgctgt acatggcctt catgaccatc 3001 atgatcaagg tgcacacctt cccactcttt gccatccggc ccatgtacct ggccatgaga 3061 cagttcaaga aagctgtgac agatgccatc atgtctcgcc gagccatccg caacatgaac 3121 accctgtatc cagatgccac cccagaggag ctccaggcaa tggacaatgt ctgcatcatc 3181 tgccgagaag agatggtgac tggtgccaag agactgccct gcaaccacat tttccatacc 3241 agctgcctgc gctcctggtt ccagcggcag cagacctgcc ccacctgccg tatggatgtc 3301 cttcgtgcat cgctgccagc gcagtcacca ccacccccgg agcctgcgga tcaggggcca 3361 ccccctgccc cccacccccc accactcttg cctcagcccc ccaacttccc ccagggcctc 3421 ctgcctcctt ttcctccagg catgttccca ctgtggcccc ccatgggccc ctttccacct 3481 gtcccgcctc cccccagctc aggagaggct gtggctcctc catccaccag tgcagcagcc 3541 ctttctcggc ccagtggagc agctacaacc acagctgctg gcaccagtgc tactgctgct 3601 tctgccacag catctggccc aggctctggc tctgccccag aggctggccc tgcccctggt 3661 ttccccttcc ctcctccctg gatgggtatg cccctgcctc caccctttgc cttcccccca 3721 atgcctgtgc cccctgcggg ctttgctggg ctgaccccag aggagctacg agctctggag 3781 ggccatgagc ggcagcacct ggaggcccgg ctgcagagcc tgcgtaacat ccacacactg 3841 ctggacgccg ccatgctgca gatcaaccag tacctcaccg tgctggcctc cttggggccc 3901 ccccggcctg ccacttcagt caactccact gaggagactg ccactacagt tgttgctgct 3961 gcctcctcca ccagcatccc tagctcagag gccacgaccc caaccccagg agcctcccca 4021 ccagcccctg aaatggaaag gcctccagct cctgagtcag tgggcacaga ggagatgcct 4081 gaggatggag agcccgatgc agcagagctc cgccggcgcc gcctgcagaa gctggagtct 4141 cctgttgccc acggatccga ctacaaagac catgacggtg attataaaga tcatgacatc 4201 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc 4261 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 4321 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 4381 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4441 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4501 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4561 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4621 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 4681 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 4741 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa 4801 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 4861 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 4921 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 4981 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5041 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 5101 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5161 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5221 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 5281 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5341 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc 5401 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5461 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 5521 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 5581 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 5641 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 5701 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 5761 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 5821 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5881 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 5941 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 6001 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 6061 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 6121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 6181 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 6241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 6301 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 6361 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6421 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6481 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 6541 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 6601 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 6661 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6721 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6781 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7501 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8821 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 8881 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 8941 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9001 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9061 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9121 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9181 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9241 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //