LOCUS V028264 10085 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028264 VERSION V028264 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4086 /label="STIP1(NM_001282652)" /note="STIP1(NM_001282652)" /gene="STIP1" CDS 4093..4119 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4123..4149 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4153..4179 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4244..4455 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4468..4736 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4765..4774 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4795..5457 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5527..5580 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5581..6180 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6181..6768 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6842..7075 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7147..7268 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7287..7422 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7460..7476) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7484..7500 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7508..7538) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7553..7574 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7862..8450) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8621..9481) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9482..9586) /gene="bla" /label="AmpR promoter" primer_bind 10060..10076 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agtccggcag cccaatggga 2341 gaggtggaaa tttccagaac gatcagaacc aatgggcgcg gccagcgcgg ctacgattgg 2401 cagtgcaaaa gaccaatccg tgtcgcagaa gttcgctcct ccctccattc gtggagcctg 2461 agatgggtca atgagctgaa ggagaaaggc aacaaggccc tgagcgtggg taacatcgat 2521 gatgccttac agtgctactc cgaagctatt aagctggatc cccacaacca cgtgctgtac 2581 agcaaccgtt ctgctgccta tgccaagaaa ggagactacc agaaggctta tgaggatggc 2641 tgcaagactg tcgacctaaa gcctgactgg ggcaagggct attcacgaaa agcagcagct 2701 ctagagttct taaaccgctt tgaagaagcc aagcgaacct atgaggaggg cttaaaacac 2761 gaggcaaata accctcaact gaaagagggt ttacagaata tggaggccag gttggcagag 2821 agaaaattca tgaacccttt caacatgcct aatctgtatc agaagttgga gagtgatccc 2881 aggacaagga cactactcag tgatcctacc taccgggagc tgatagagca gctacgaaac 2941 aagccttctg acctgggcac gaaactacaa gatccccgga tcatgaccac tctcagcgtc 3001 ctccttgggg tcgatctggg cagtatggat gaggaggaag agattgcaac acctccacca 3061 ccaccccctc ccaaaaagga gaccaagcca gagccaatgg aagaagatct tccagagaat 3121 aagaagcagg cactgaaaga aaaagagctg gggaacgatg cctacaagaa gaaagacttt 3181 gacacagcct tgaagcatta cgacaaagcc aaggagctgg accccactaa catgacttac 3241 attaccaatc aagcagcggt atactttgaa aagggcgact acaataagtg ccgggagctt 3301 tgtgagaagg ccattgaagt ggggagagaa aaccgagaag actatcgaca gattgccaaa 3361 gcatatgctc gaattggcaa ctcctacttc aaagaagaaa agtacaagga tgccatccat 3421 ttctataaca agtctctggc agagcaccga accccagatg tgctcaagaa atgccagcag 3481 gcagagaaaa tcctgaagga gcaagagcgg ctggcctaca taaaccccga cctggctttg 3541 gaggagaaga acaaaggcaa cgagtgtttt cagaaagggg actatcccca ggccatgaag 3601 cattatacag aagccatcaa aaggaacccg aaagatgcca aattatacag caatcgagct 3661 gcctgctaca ccaaactcct ggagttccag ctggcactca aggactgtga ggaatgtatc 3721 cagctggagc cgaccttcat caagggttat acacggaaag ccgctgcgct ggaagcgatg 3781 aaggactaca ccaaagccat ggatgtgtac cagaaggcgc tagacctgga ctccagctgt 3841 aaggaggcgg cagacggcta ccagcgctgt atgatggcgc agtacaaccg gcacgacagc 3901 cccgaagatg tgaagcgacg agccatggcc gaccctgagg tgcagcagat catgagtgac 3961 ccagccatgc gccttatcct ggaacagatg cagaaggacc cccaggcact cagcgaacac 4021 ttaaagaatc ctgtaatagc acagaagatc cagaagctga tggatgtggg tctgattgca 4081 attcggctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct 4141 gattatgcta gctatcctta tgatgtccca gactacgcat agggatccct gcgatatcca 4201 gcgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4261 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4321 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4381 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4441 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4501 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4561 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4621 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4681 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4741 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4801 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 4861 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 4921 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 4981 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5041 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5101 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5161 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5221 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5281 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5341 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5401 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5461 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5521 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5581 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5641 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5701 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5761 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5821 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5881 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5941 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6001 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6061 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6121 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6181 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6241 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6301 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6361 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6421 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6481 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6541 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 6601 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6661 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctc 6721 cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct ggtaccttta 6781 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6841 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6901 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6961 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 7021 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 7081 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 7141 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 7201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 7261 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 7321 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 7381 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 7441 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7501 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7561 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7621 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 8041 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 8101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 8161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 8221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8281 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8581 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8701 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8761 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 9001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 9061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 9121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 9181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9481 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9541 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9601 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9661 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9841 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9901 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9961 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 10021 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 10081 agctg //