LOCUS       V027306                10604 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027306
VERSION     V027306
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4698
                     /label="STAT5A(NM_001288718)"
                     /note="STAT5A(NM_001288718)"
                     /gene="STAT5A"
     promoter        4763..4974
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4987..5255
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5284..5293
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5314..5976
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6046..6099
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6100..6699
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6700..7287
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7361..7594
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7666..7787
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7806..7941
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7979..7995)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8003..8019
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8027..8057)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8072..8093
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8381..8969)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9140..10000)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10001..10105)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10579..10595
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgggctggat ccaggcccag
     2341 cagctgcagg gagacgcgct gcgccagatg caggtgctgt acggccagca cttccccatc
     2401 gaggtccggc actacttggc ccagtggatt gagagccagc catgggatgc cattgacttg
     2461 gacaatcccc aggacagagc ccaagccacc cagctcctgg agggcctggt gcaggagctg
     2521 cagaagaagg cggagcacca ggtgggggaa gatgggtttt tactgaagat caagctgggg
     2581 cactacgcca cgcagctcca gaaaacatat gaccgctgcc ccctggagct ggtccgctgc
     2641 atccggcaca ttctgtacaa tgaacagagg ctggtccgag aagccaacaa ttgcagctct
     2701 ccggctggga tcctggttga cgccatgtcc cagaagcacc ttcagatcaa ccagacattt
     2761 gaggagctgc gactggtcac gcaggacaca gagaatgagc tgaagaaact gcagcagact
     2821 caggagtact tcatcatcca gtaccaggag agcctgagga tccaagctca gtttgcccag
     2881 ctggcccagc tgagccccca ggagcgtctg agccgggaga cggccctcca gcagaagcag
     2941 gtgtctctgg aggcctggtt gcagcgtgag gcacagacac tgcagcagta ccgcgtggag
     3001 ctggccgaga agcaccagaa gaccctgcag ctgctgcgga agcagcagac catcatcctg
     3061 gatgacgagc tgatccagtg gaagcggcgg cagcagctgg ccgggaacgg cgggcccccc
     3121 gagggcagcc tggacgtgct acagtcctgg tgtgagaagt tggccgagat catctggcag
     3181 aaccggcagc agatccgcag ggctgagcac ctctgccagc agctgcccat ccccggccca
     3241 gtggaggaga tgctggccga ggtcaacgcc accatcacgg acattatctc agccctggtg
     3301 accagcacat tcatcattga gaagcagcct cctcaggtcc tgaagaccca gaccaagttt
     3361 gcagccaccg tacgcctgct ggtgggcggg aagctgaacg tgcacatgaa tcccccccag
     3421 gtgaaggcca ccatcatcag tgagcagcag gccaagtctc tgcttaaaaa tgagaacacc
     3481 cgcaacgagt gcagtggtga gatcctgaac aactgctgcg tgatggagta ccaccaagcc
     3541 acgggcaccc tcagtgccca cttcaggaac atgtcactga agaggatcaa gcgtgctgac
     3601 cggcggggtg cagagtccgt gacagaggag aagttcacag tcctgtttga gtctcagttc
     3661 agtgttggca gcaatgagct tgtgttccag gtgaagactc tgtccctacc tgtggttgtc
     3721 atcgtccacg gcagccagga ccacaatgcc acggctactg tgctgtggga caatgccttt
     3781 gctgagccgg gcagggtgcc atttgccgtg cctgacaaag tgctgtggcc gcagctgtgt
     3841 gaggcgctca acatgaaatt caaggccgaa gtgcagagca accggggcct gaccaaggag
     3901 aacctcgtgt tcctggcgca gaaactgttc aacaacagca gcagccacct ggaggactac
     3961 agtggcctgt ccgtgtcctg gtcccagttc aacagggaga acttgccggg ctggaactac
     4021 accttctggc agtggtttga cggggtgatg gaggtgttga agaagcacca caagccccac
     4081 tggaatgatg gggccatcct aggttttgtg aataagcaac aggcccacga cctgctcatc
     4141 aacaagcccg acgggacctt cttgttgcgc tttagtgact cagaaatcgg gggcatcacc
     4201 atcgcctgga agtttgactc cccggaacgc aacctgtgga acctgaaacc attcaccacg
     4261 cgggatttct ccatcaggtc cctggctgac cggctggggg acctgagcta tctcatctat
     4321 gtgtttcctg accgccccaa ggatgaggtc ttctccaagt actacactcc tgtgctggct
     4381 aaagctgttg atggatatgt gaaaccacag atcaagcaag tggtccctga gtttgtgaat
     4441 gcatctgcag atgctggggg cagcagcgcc acgtacatgg accaggcccc ctccccagct
     4501 gtgtgccccc aggctcccta taacatgtac ccacagaacc ctgaccatgt actcgatcag
     4561 gatggagaat tcgacctgga tgagaccatg gatgtggcca ggcacgtgga ggaactctta
     4621 cgccgaccaa tggacagtct tgactcccgc ctctcgcccc ctgccggtct tttcacctct
     4681 gccagaggct ccctctcatg aggatccctg cgatatccag cgcggccgcg aaggatctgc
     4741 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4801 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4861 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4921 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     4981 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     5041 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     5101 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     5161 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     5221 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     5281 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc
     5341 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag
     5401 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac
     5461 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac
     5521 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag
     5581 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg
     5641 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac
     5701 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg
     5761 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg
     5821 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc
     5881 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag
     5941 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat
     6001 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt
     6061 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg
     6121 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6181 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6241 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6301 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6361 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6421 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6481 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6541 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6601 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6661 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa
     6721 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6781 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6841 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6901 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6961 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7021 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7081 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     7141 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7201 cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt
     7261 cggatctccc tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     7321 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7381 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7441 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7501 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7561 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7621 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7681 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7741 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7801 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7921 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
     7981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     8401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8821 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10141 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    10201 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    10261 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    10321 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    10381 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    10441 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10501 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10561 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
