LOCUS V044964 9247 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044964 VERSION V044964 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..4101 /label="ST6GALNAC1(NM_018414)" /note="ST6GALNAC1(NM_018414)" /gene="ST6GALNAC1" CDS 4108..4173 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4217..4428 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4441..4709 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4736..5335 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5342..5930 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6004..6237 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6309..6430 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6449..6584 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6622..6638) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6646..6662 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6670..6700) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6715..6736 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7024..7612) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7783..8643) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8644..8748) /gene="bla" /label="AmpR promoter" primer_bind 9222..9238 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgaggtcc tgcctgtgga gatgcaggca cctgagccaa 2341 ggcgtccagt ggtccttgct tctggctgtc ctggtcttct ttctcttcgc cttgccctct 2401 tttattaagg agcctcaaac aaagccttcc aggcatcaac gcacagagaa cattaaagaa 2461 aggtctctac agtccctggc aaagcctaag tcccaggcac ccacaagggc aaggaggaca 2521 accatctatg cagagccagt gccagagaac aatgccctca acacacaaac ccagcccaag 2581 gcccacacca ccggagacag aggaaaggag gccaaccagg caccgccgga ggagcaggac 2641 aaggtgcccc acacagcaca gagggcagca tggaagagcc cagaaaaaga gaaaaccatg 2701 gtgaacacac tgtcacccag agggcaagat gcagggatgg cctctggcag gacagaggca 2761 caatcatgga agagccagga cacaaagacg acccaaggaa atgggggcca gaccaggaag 2821 ctgacggcct ccaggacggt gtcagagaag caccagggca aagcggcaac cacagccaag 2881 acgctcattc ccaaaagtca gcacagaatg ctggctccca caggagcagt gtcaacaagg 2941 acgagacaga aaggagtgac cacagcagtc atcccaccta aggagaagaa acctcaggcc 3001 accccacccc ctgccccttt ccagagcccc acgacgcaga gaaaccaaag actgaaggcc 3061 gccaacttca aatctgagcc tcggtgggat tttgaggaaa aatacagctt cgaaatagga 3121 ggccttcaga cgacttgccc tgactctgtg aagatcaaag cctccaagtc gctgtggctc 3181 cagaaactct ttctgcccaa cctcactctc ttcctggact ccagacactt caaccagagt 3241 gagtgggacc gcctggaaca ctttgcacca ccctttggct tcatggagct caactactcc 3301 ttggtgcaga aggtcgtgac acgcttccct ccagtgcccc agcagcagct gctcctggcc 3361 agcctccccg ctgggagcct ccggtgcatc acctgtgccg tggtgggcaa cgggggcatc 3421 ctgaacaact cccacatggg ccaggagata gacagtcacg actacgtgtt ccgattgagc 3481 ggagctctca ttaaaggcta cgaacaggat gtggggactc ggacatcctt ctacggcttt 3541 accgccttct ccctgaccca gtcactcctt atattgggca atcggggttt caagaacgtg 3601 cctcttggga aggacgtccg ctacttgcac ttcctggaag gcacccggga ctatgagtgg 3661 ctggaagcac tgcttatgaa tcagacggtg atgtcaaaaa accttttctg gttcaggcac 3721 agaccccagg aagcttttcg ggaagccctg cacatggaca ggtacctgtt gctgcaccca 3781 gactttctcc gatacatgaa gaacaggttt ctgaggtcta agaccctgga tggtgcccac 3841 tggaggatat accgccccac cactggggcc ctcctgctgc tcactgccct tcagctctgt 3901 gaccaggtga gtgcttatgg cttcatcact gagggccatg agcgcttttc tgatcactac 3961 tatgatacat catggaagcg gctgatcttt tacataaacc atgacttcaa gctggagaga 4021 gaagtctgga agcggctaca cgatgaaggg ataatccggc tgtaccagcg tcctggtccc 4081 ggaactgcca aagccaagaa cggatccgac tacaaagacc atgacggtga ttataaagat 4141 catgacatcg actacaagga tgacgatgac aagtagcggc cgcgaaggat ctgcgatcgc 4201 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 4261 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4321 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4381 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4441 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4501 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4561 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4621 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4681 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagatgac 4741 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac 4801 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca 4861 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 4921 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 4981 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 5041 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 5101 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 5161 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 5221 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 5281 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 5341 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 5581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 5761 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5881 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt 5941 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6001 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 6061 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6121 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6181 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6241 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6301 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6421 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 6481 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 6541 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 6601 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 6661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8761 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8821 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8881 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8941 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9001 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9061 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9121 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9181 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9241 caagctg //