LOCUS       V034570                10906 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034570
VERSION     V034570
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2288..2297
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2297..2362
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             2369..5791
                     /label="SREBF2(NM_004599)"
                     /note="SREBF2(NM_004599)"
                     /gene="SREBF2"
     CDS             5792..5809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        5876..6087
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6100..6368
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6395..6994
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7001..7589
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7663..7896
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7968..8089
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8108..8243
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8281..8297)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8305..8321
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8329..8359)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8374..8395
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8683..9271)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9442..10302)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10303..10407)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10881..10897
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatggact acaaagacca tgacggtgat tataaagatc atgacatcga
     2341 ctacaaggat gacgatgaca aggaattcat ggacgacagc ggcgagctgg gtggtctgga
     2401 gaccatggag accctcacgg agctgggcga cgagctgacc ctgggagaca tcgacgagat
     2461 gctgcaattt gtcagtaatc aagtgggaga gttccctgac ttgttttcag aacagctgtg
     2521 tagctccttt cctggcagtg gtggtagtgg tagcagcagc ggcagcagtg gcagcagcag
     2581 cagcagcagc aatggcaggg gcagcagcag cggagctgtg gacccttcag tgcaacggtc
     2641 attcacccag gtcacattac cttccttctc tccctcggcg gcctccccac aggctccaac
     2701 tctgcaagtc aaggtttctc ccacctcagt tcccaccaca cccagggcaa ctcctattct
     2761 tcagccccgc ccccagcccc agcctcaacc tcaaactcag ctgcaacaac agacggtaat
     2821 gatcacgcca acattcagca ccactccgca gacgaggatc atccagcagc ctttgatata
     2881 ccagaatgca gctactagct ttcaagtcct tcagcctcaa gtccaaagcc tggtgacatc
     2941 ctcccaggta cagccggtca ccattcagca gcaggtgcag acagtacagg cccagcgggt
     3001 gctgacacaa acggccaatg gcacgctgca gacccttgcc ccggctacgg tgcagacagt
     3061 tgctgcgcca caggtgcagc aggtcccggt cctggtccag cctcagatca tcaagacaga
     3121 ttcccttgtt ttgaccacac tgaagacaga tggcagccct gttatggctg cggtccagaa
     3181 cccggccctc accgccctca ccacccctat ccagacggct gcccttcaag taccaaccct
     3241 ggtgggcagc agtgggacca ttctgaccac aatgcctgta atgatggggc aagagaaagt
     3301 gcccattaag caggtacctg ggggagtcaa gcagcttgag ccccccaaag aaggagaaag
     3361 gcggacaacc cataatatca ttgagaaacg atatcgctcc tccatcaatg acaaaatcat
     3421 cgaattgaaa gacctggtca tggggacaga cgccaagatg cacaagtctg gcgttctgag
     3481 gaaggccatt gattacatca aatacttgca gcaggtcaat cataaactgc gccaggagaa
     3541 catggtgctg aagctggcaa atcaaaagaa caagcttcta aagggcatcg acctaggcag
     3601 tctggtggac aatgaggtgg acctgaagat cgaggacttt aatcagaatg tccttctgat
     3661 gtccccccca gcctctgact cagggtccca ggctggcttc tctccctact ccattgactc
     3721 tgagccagga agccctctat tggatgatgc aaaggtcaaa gatgagccag actctcctcc
     3781 tgtggcgctg ggcatggtag accgctcacg gattcttctg tgtgtcctca ccttcctgtg
     3841 cctctccttt aaccccctga cttccctgct gcagtgggga ggggcccacg actctgacca
     3901 gcacccacac tcaggctctg gccgcagtgt cctgtcattc gagtcaggtt ctgggggctg
     3961 gtttgactgg atgatgccta ctcttctctt atggctggta aatggtgtga ttgtcctgag
     4021 cgtctttgtg aagctgctgg ttcatgggga gccagtgatc cggccacact cgcgctcctc
     4081 ggtcaccttc tggaggcacc ggaaacaggc agatctggat ctcgccagag gagattttgc
     4141 agctgctgcc ggcaacctac aaacctgcct ggcagttttg ggccgggcac tgcccacctc
     4201 ccgcctggac ctggcctgca gcctctcctg gaacgtgatc cgctacagcc tgcagaagct
     4261 acgcctggtg cgctggctgc tcaagaaagt cttccagtgc cggcgggcca cgccagccac
     4321 tgaggcaggc tttgaagacg aagctaagac cagcgcccgg gatgcggctc tggcctatca
     4381 ccggctgcac cagctgcaca tcacagggaa gcttcctgca ggatccgcct gttccgatgt
     4441 acacatggcg ttgtgtgccg tgaacctggc tgaatgtgca gaggagaaga tcccaccgag
     4501 cacactggtt gagatccatc tgactgctgc catggggctc aagacccggt gtggaggcaa
     4561 gctgggcttc ctggccagct acttcctcag ccgagcccag agcctgtgtg gccccgagca
     4621 cagtgctgtt cctgactccc tgcgctggct ctgccacccc ctgggccaga agtttttcat
     4681 ggagcggagc tggtctgtga agtcagctgc caaggagagt ctatactgtg cccagaggaa
     4741 cccagctgac cccattgcgc aggtccacca ggccttctgc aagaacctgc tggagcgagc
     4801 tatagagtcc ttggtgaaac ctcaggccaa gaagaaggct ggagaccagg aagaagagag
     4861 ctgtgaattc tccagtgctc tggagtactt gaaattactt cattcttttg tggactctgt
     4921 gggggttatg agccccccac tctccaggag ctccgtgctc aagtccgccc tgggtccaga
     4981 catcatctgt cggtggtgga cgtctgcaat cactgtggcc atcagctggc tccagggaga
     5041 cgatgcagct gtgcgctctc attttaccaa agtggaacgc atccccaagg ccctggaagt
     5101 gacagagagc cccctggtga aggccatctt ccatgcctgc agagccatgc atgcctcact
     5161 ccctgggaaa gcagatgggc agcagagttc cttctgccat tgcgagaggg ccagtggcca
     5221 cctatggagc agcctcaacg tcagtggggc cacctctgac cctgccctca accacgtggt
     5281 ccagctgctc acctgtgacc tgctactgtc gctacggaca gcgctctggc aaaaacaggc
     5341 cagtgccagc caggctgtgg gggagaccta ccacgcgtca ggcgctgaac tggcgggctt
     5401 ccaacgggac ctgggcagcc tgcgcaggct ggcacacagc ttccgcccag cataccgcaa
     5461 ggtgttcctg catgaagcca ccgtgcgcct gatggcagga gccagcccca cccgcaccca
     5521 ccagctgctg gaacacagcc tgcggcggcg caccacgcag agcaccaagc acggagaggt
     5581 ggatgcctgg cccggccagc gagagcgggc caccgccatc ctgctggcct gccgccacct
     5641 gcccctctcc ttcctctcct ccccgggcca gcgggcagtg ctgctggccg aagctgcccg
     5701 caccctggag aaggtgggcg accggcgctc ctgcaacgac tgccagcaga tgattgttaa
     5761 gctgggtggt ggcactgcca ttgccgcctc ccaccaccac caccaccact aggatccctc
     5821 gagagatcag tgtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca
     5881 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt
     5941 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     6001 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     6061 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca
     6121 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct
     6181 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg
     6241 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg
     6301 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt
     6361 tacagatcca agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg
     6421 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
     6481 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
     6541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6601 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6661 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6841 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6961 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa
     7021 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7081 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7141 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7201 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7261 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7321 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7381 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7441 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7501 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7561 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     7621 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7681 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7861 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7921 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8101 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8161 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8221 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     8281 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8341 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8401 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8461 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9121 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10441 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10501 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10561 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10621 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10681 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10741 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10801 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10861 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
