LOCUS       V041876                11446 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041876
VERSION     V041876
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5472
                     /label="SMARCA5(NM_003601)"
                     /note="SMARCA5(NM_003601)"
                     /gene="SMARCA5"
     CDS             5479..5544
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5588..5799
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5812..6080
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6109..6118
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6139..6801
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6871..6924
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6925..7524
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7525..8113
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8187..8420
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8492..8613
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8653..8788
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8821..8837)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8845..8861
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8869..8899)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8914..8935
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9223..9811)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9982..10842)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10843..10947)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11421..11437
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgtccgcggc cgagcctccg
     2341 ccacccccgc ctcccgagag cgcgccttcc aagcccgcag cctcgatcgc cagcggcggg
     2401 agcaacagca gcaacaaagg cggccccgaa ggcgtcgcgg cgcaggcggt tgcgtctgcg
     2461 gccagcgctg gtcccgcaga cgccgagatg gaggaaatat ttgatgatgc gtcacctgga
     2521 aagcaaaagg aaatccaaga accagatcct acctatgaag aaaaaatgca aactgaccgg
     2581 gcaaatagat tcgagtattt attaaagcag acagaacttt ttgcacattt cattcaacct
     2641 gctgctcaga agactccaac ttcacctttg aagatgaaac cagggcgccc acgaataaaa
     2701 aaagatgaga agcagaactt actatccgtt ggcgattacc gacaccgtag aacagagcaa
     2761 gaggaggatg aagagctatt aacagaaagc tccaaagcaa ccaatgtttg cactcgattt
     2821 gaagactctc catcgtatgt aaaatggggt aaactgagag attatcaggt ccgaggatta
     2881 aactggctca tttctttgta tgagaatggc atcaatggta tccttgcaga tgaaatgggc
     2941 ctaggaaaga ctcttcaaac aatttctctt cttgggtaca tgaaacatta tagaaacatt
     3001 cctgggcctc atatggtttt ggttcctaag tctacattac acaactggat gagtgaattc
     3061 aagagatggg taccaacact tagatctgtt tgtttgatag gagataaaga acaaagagct
     3121 gcttttgtca gagacgtttt attaccggga gaatgggatg tatgtgtaac atcttatgaa
     3181 atgcttatta aagagaagtc tgtgttcaaa aaatttaatt ggagatactt agtaatagat
     3241 gaagctcaca ggatcaaaaa tgaaaaatct aagttgtcag aaatagtgag ggaattcaag
     3301 actacaaata gactattatt aactggaaca cctcttcaga acaacttgca tgagctgtgg
     3361 tcacttctta actttctgtt gccagatgtg tttaattcag cagatgactt tgattcctgg
     3421 tttgatacaa acaactgcct tggggatcaa aaactagttg agaggcttca tatggttttg
     3481 cgtccattcc tccttcgtcg aattaaggct gatgttgaaa agagtttgcc tccaaagaag
     3541 gaagtaaaaa tctatgtggg cctcagcaaa atgcaaaggg aatggtatac tcggatatta
     3601 atgaaggata tagatatact caactcagca ggcaagatgg acaaaatgag gttattgaac
     3661 atcctaatgc agttgagaaa atgttgtaat catccatatc tctttgatgg agcagaacct
     3721 ggtccacctt atacaacaga tatgcatcta gtaaccaaca gtggcaaaat ggtggtttta
     3781 gacaagctgc tccctaagtt aaaagaacaa ggttcacgag tactaatctt cagtcaaatg
     3841 acaagggtat tggacatttt ggaagattat tgcatgtgga gaaattatga gtactgcagg
     3901 ttggatggtc agacacccca tgatgagaga caagactcca tcaatgcata caatgaacca
     3961 aacagcacaa agtttgtttt catgttaagc acgcgtgctg gtggtcttgg catcaatctt
     4021 gcgactgctg atgtagtaat tttgtatgat tctgattgga atccccaagt agatcttcag
     4081 gctatggacc gagcacatag aattgggcag actaagacag tcagagtgtt ccgctttata
     4141 actgataaca ctgtagaaga aagaatagta gaacgtgctg agatgaaact cagactggat
     4201 tcaatagtca ttcaacaagg gaggcttgtg gatcagaatc tgaacaaaat tgggaaagat
     4261 gaaatgcttc aaatgattag acatggagca acacatgtgt ttgcttcaaa ggaaagtgag
     4321 atcactgatg aagatatcga tggtattttg gaaagaggtg caaagaagac tgcagagatg
     4381 aatgaaaagc tctccaagat gggcgaaagt tcacttagaa actttacaat ggatacagag
     4441 tcaagtgttt ataacttcga aggagaagac tatagagaaa aacaaaagat tgcattcaca
     4501 gagtggattg aaccacctaa acgagaaaga aaagccaact atgccgttga tgcatatttc
     4561 agggaagctc ttcgtgttag tgaacctaaa gcacccaagg ctcctcgacc tccaaaacaa
     4621 cccaatgttc aggatttcca gttctttcct ccacgtttat ttgaattact ggaaaaagaa
     4681 attctgtttt acagaaaaac tattgggtac aaggtacctc gaaatcctga gctgcctaat
     4741 gcagcacagg cacaaaaaga agaacagctt aaaattgatg aagctgaatc ccttaatgat
     4801 gaagagttag aggaaaaaga gaagcttcta acacagggat ttaccaattg gaataagaga
     4861 gattttaacc agtttatcaa agctaatgag aagtggggtc gtgatgatat tgaaaatata
     4921 gcaagagaag tagaaggcaa aactccagaa gaagtcattg aatattcagc tgtgttttgg
     4981 gaaaggtgca acgagctcca ggacatagag aagattatgg ctcagattga aaggggagag
     5041 gcgagaattc aaagaagaat aagcatcaag aaagcacttg acacaaagat tggacggtac
     5101 aaagcacctt ttcatcagct gagaatatca tatggtacta acaaaggaaa aaactatact
     5161 gaagaagaag atcgttttct gatttgtatg cttcacaaac ttggatttga caaagaaaat
     5221 gtttatgatg aattgcgaca gtgtattcgc aactctcctc agttcagatt tgactggttt
     5281 cttaagtcca gaactgcaat ggagctccag aggagatgta ataccttaat tactttgatt
     5341 gaaagagaaa acatggaact agaagaaaag gagaaggcag agaaaaagaa acgaggacca
     5401 aagccttcaa cacagaaacg taaaatggat ggcgcacctg atggtcgagg aagaaaaaag
     5461 aagctgaaac taggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5521 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5581 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5641 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5701 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5761 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5821 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5881 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5941 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6001 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6061 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6121 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6181 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6241 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6301 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6361 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     6421 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     6481 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     6541 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     6601 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     6661 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     6721 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     6781 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     6841 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     6901 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     6961 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7021 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7081 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7141 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7201 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7261 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7321 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7381 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7441 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7501 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     7561 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7621 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7681 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7741 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7801 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7861 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7921 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7981 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8041 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8101 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8161 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     8221 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8281 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8341 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8401 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8461 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8521 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8581 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8641 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8701 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8761 aagtagtgag gaggcttttt tggaggccta gacttttgca gagaccaaat tcgtaatcat
     8821 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8881 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8941 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9001 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10981 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11041 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11101 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11161 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11221 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11281 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11341 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11401 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
