LOCUS       V033159                10631 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033159
VERSION     V033159
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5463
                     /label="SMARCA1(NM_003069)"
                     /note="SMARCA1(NM_003069)"
                     /gene="SMARCA1"
     CDS             5470..5496
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5500..5526
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5530..5556
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5601..5812
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5825..6093
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6120..6719
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6726..7314
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7388..7621
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7693..7814
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7833..7968
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8006..8022)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8030..8046
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8054..8084)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8099..8120
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8408..8996)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9167..10027)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10028..10132)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10606..10622
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggagcag gacactgccg cagtggcagc caccgtggca
     2341 gccgcggatg cgaccgccac tatcgtggtc atagaggacg agcagcccgg gccgtccacc
     2401 tctcaggagg agggagcggc cgccgcggcc accgaagcca ccgcggccac ggagaagggc
     2461 gagaagaaga aggagaaaaa cgtttcttca tttcaactca aacttgctgc taaagcgcct
     2521 aaatctgaaa aggaaatgga cccagaatat gaagagaaaa tgaaagccga ccgagcaaag
     2581 agatttgaat ttttactgaa gcagacagaa ctttttgcac atttcattca gccttcagca
     2641 cagaaatctc caacatctcc actgaacatg aaattgggac gtccccgaat aaagaaagat
     2701 gaaaagcaga gcttaatttc tgctggagac taccgccata ggcgcacaga gcaagaagaa
     2761 gatgaagagc tactgtctga gagtcggaaa acatctaatg tgtgtattag atttgaggtg
     2821 tcaccttcat atgtgaaagg ggggccactg agagattatc agattcgagg actgaattgg
     2881 ttgatctctt tatatgaaaa tggagtcaat ggcattttgg ctgatgaaat gggccttggg
     2941 aaaactttac aaacaattgc tttgcttggt tacctgaaac actaccgaaa tattcctgga
     3001 cctcacatgg ttttagttcc aaagtctact ttacacaact ggatgaatga atttaaacga
     3061 tgggtcccat ctctccgtgt catttgtttt gtcggagaca aggatgccag agctgctttt
     3121 attcgtgatg aaatgatgcc aggagagtgg gatgtttgcg ttacttctta tgagatggta
     3181 attaaagaaa aatctgtatt caaaaagttt cactggcgat acctggtcat tgatgaagct
     3241 cacagaataa agaatgaaaa atctaagctt tcagagattg ttcgtgagtt caagtcgact
     3301 aaccgcttgc tcctaactgg aacacctttg cagaataacc tgcatgaact gtgggcctta
     3361 ctcaactttt tattgcctga tgtctttaat tctgcagatg actttgattc ttggtttgac
     3421 actaaaaatt gtcttggtga tcaaaaactc gtggaaagac ttcatgcagt tttaaaacca
     3481 tttttgttac gccgtataaa aactgatgta gagaagagtc tgccacctaa aaaggaaata
     3541 aagatttact tggggctgag taagatgcaa cgagaatggt atacaaaaat cctgatgaaa
     3601 gatattgatg ttttaaactc ttctggcaag atggacaaga tgcgactctt aaacattctg
     3661 atgcagcttc gaaagtgttg taatcatcca tatctgtttg atggtgctga acctggtcca
     3721 ccttatacca ctgatgagca tattgtcagc aacagtggta aaatggtagt tctggataaa
     3781 ctattggcca aactcaaaga acagggttca agggttctca ttttcagcca gatgactcgc
     3841 ttgctggata ttttggaaga ttattgcatg tggcgtggtt atgagtattg tcgactggat
     3901 ggacaaaccc cgcatgaaga aagagaggat aaattcctag aagtggaatt tctgggtcaa
     3961 agggaagcaa tagaggcttt taatgctcct aatagtagca aattcatctt tatgctaagt
     4021 accagggctg gaggtctcgg aattaacctg gcaagtgctg atgtggttat actatatgat
     4081 tcagactgga acccacaggt tgatctacaa gctatggatc gagcacatcg tattggtcag
     4141 aagaaaccag tacgtgtatt ccgtctcatc actgacaaca ctgttgaaga gaggattgta
     4201 gaaagagctg agataaaact gagactcgat tcaattgtta tacaacaagg aagactcatt
     4261 gaccaacagt ctaacaagct ggcaaaagag gaaatgttac aaatgatacg gcatggagcc
     4321 acccatgttt ttgcttctaa agagagtgag ttgacagatg aagacattac aactattctg
     4381 gaaagagggg aaaagaagac tgcagagatg aatgaacgcc tgcaaaaaat gggagagtct
     4441 tctctaagaa attttagaat ggacattgaa caaagtttat acaaatttga gggagaagat
     4501 tatagagaaa aacagaagct tggcatggtg gaatggattg aacctcctaa acgagaacgc
     4561 aaagcaaact acgcagtgga tgcctacttt agagaggctt tgcgtgtcag cgagccaaag
     4621 attccaaagg ctccacggcc tccaaaacag ccaaatgttc aggattttca atttttccca
     4681 ccacgcttat ttgagctcct ggaaaaggaa attctttatt atcggaagac aataggctat
     4741 aaggttccaa ggaatcctga tatcccaaat ccagctctgg ctcaaagaga agagcaaaaa
     4801 aagattgatg gagctgaacc tcttacacca gaagagactg aagaaaagga aaaacttctc
     4861 acacaaggtt tcacaaactg gactaaacga gattttaacc agtttattaa agctaatgag
     4921 aaatatggaa gagatgacat tgataacata gctcgagagg tagagggcaa atcccctgag
     4981 gaggtcatgg agtattcagc tgtattttgg gaacgttgca atgaattaca ggacattgag
     5041 aaaattatgg ctcaaattga acgtggagaa gcaagaattc aacgaaggat cagtatcaag
     5101 aaagccctgg atgccaaaat tgcaagatac aaggctccat ttcatcagtt gcgcattcag
     5161 tatggaacca gcaaaggaaa gaactatact gaggaagaag atagattctt gatttgtatg
     5221 ttacacaaaa tgggctttga tagagaaaat gtatatgaag aattaagaca gtgtgtacga
     5281 aatgctcccc agtttagatt tgactggttt atcaagtcta ggactgccat ggaattccag
     5341 agacgctgta acactctgat ttcattgatt gagaaagaaa atatggaaat tgaggaaaga
     5401 gagagagcag aaaagaagaa acgggcaact aaaactccaa tggtaaaatt ttcagcattt
     5461 tccggatcct acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat
     5521 tatgctagct atccttatga tgtcccagac tacgcatagg cggccgcgaa ggatctgcga
     5581 tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     5641 gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag
     5701 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     5761 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct
     5821 tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg
     5881 ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt
     5941 ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct
     6001 acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt
     6061 tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga
     6121 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6181 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6241 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6301 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6361 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     6421 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6481 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6541 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     6601 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6661 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     6721 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6781 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6841 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6901 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6961 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7021 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7081 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7141 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7201 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7261 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta
     7321 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7381 gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact
     7441 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7501 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7561 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7621 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7681 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7741 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7801 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg
     7861 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7921 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
     7981 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8821 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
    10201 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    10261 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    10321 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    10381 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10441 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10501 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10561 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10621 gtgccaagct g
//
