LOCUS       V031709                10417 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031709
VERSION     V031709
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4443
                     /label="SLCO2B1(NM_007256)"
                     /note="SLCO2B1(NM_007256)"
                     /gene="SLCO2B1"
     CDS             4450..4515
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4559..4770
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4783..5051
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5080..5089
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5110..5772
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5842..5895
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5896..6495
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6496..7084
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7158..7391
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7463..7584
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7624..7759
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7792..7808)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7816..7832
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7840..7870)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7885..7906
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8194..8782)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8953..9813)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9814..9918)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10392..10408
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg gacccaggat agggccagcg
     2341 ggtgaggtac cccaggtacc agacaaggaa accaaagcca caatgggcac agaaaacaca
     2401 cctggaggca aagccagccc agaccctcag gacgtgcggc caagtgtgtt ccataacatc
     2461 aagctgttcg ttctgtgcca cagcctgctg cagctggcgc agctcatgat ctccggctac
     2521 ctaaagagct ccatctccac agtggagaag cgcttcggcc tctccagcca gacgtcgggg
     2581 ctgctggcct ccttcaacga ggtggggaac acagccttga ttgtgtttgt gagctatttt
     2641 ggcagccggg tgcaccgacc ccgaatgatt ggctatgggg ctatccttgt ggccctggcg
     2701 ggcctgctca tgactctccc gcacttcatc tcggagccat accgctacga caacaccagc
     2761 cctgaggata tgccacagga cttcaaggct tccctgtgcc tgcccacaac ctcggcccca
     2821 gcctcggccc cctccaatgg caactgctca agctacacag aaacccagca tctgagtgtg
     2881 gtggggatca tgttcgtggc acagaccctg ctgggcgtgg gcggggtgcc cattcagccc
     2941 tttggcatct cctacatcga tgactttgcc cacaacagca actcgcccct ctacctcggg
     3001 atcctgtttg cagtgaccat gatggggcca ggcctggcct ttgggctggg cagcctcatg
     3061 ctgcgccttt atgtggacat taaccagatg ccagaaggtg gtatcagcct gaccataaag
     3121 gacccccgat gggtgggtgc ctggtggctg ggtttcctca tcgctgccgg tgcagtggcc
     3181 ctggctgcca tcccctactt cttcttcccc aaggaaatgc ccaaggaaaa acgtgagctt
     3241 cagtttcggc gaaaggtctt agcagtcaca gactcacctg ccaggaaggg caaggactct
     3301 ccctctaagc agagccctgg ggagtccacg aagaagcagg atggcctagt ccagattgca
     3361 ccaaacctga ctgtgatcca gttcattaaa gtcttcccca gggtgctgct gcagacccta
     3421 cgccacccca tcttcctgct ggtggtcctg tcccaggtat gcttgtcatc catggctgcg
     3481 ggcatggcca ccttcctgcc caagttcctg gagcgccagt tttccatcac agcctcctac
     3541 gccaacctgc tcatcggctg cctctccttc ccttcggtca tcgtgggcat cgtggtgggt
     3601 ggcgtcctgg tcaagcggct ccacctgggc cctgtgggat gcggtgccct ttgcctgctg
     3661 gggatgctgc tgtgcctctt cttcagcctg ccgctcttct ttatcggctg ctccagccac
     3721 cagattgcgg gcatcacaca ccagaccagt gcccaccctg ggctggagct gtctccaagc
     3781 tgcatggagg cctgctcctg cccattggac ggctttaacc ctgtctgcga ccccagcact
     3841 cgtgtggaat acatcacacc ctgccacgca ggctgctcaa gctgggtggt ccaggatgct
     3901 ctggacaaca gccaggtttt ctacaccaac tgcagctgcg tggtggaggg caaccccgtg
     3961 ctggcaggat cctgcgactc aacgtgcagc catctggtgg tgcccttcct gctcctggtc
     4021 agcctgggct cggccctggc ctgtctcacc cacacaccct ccttcatgct catcctaaga
     4081 ggagtgaaga aagaagacaa gactttggct gtgggcatcc agttcatgtt cctgaggatt
     4141 ttggcctgga tgcccagccc cgtgatccac ggcagcgcca tcgacaccac ctgtgtgcac
     4201 tgggccctga gctgtgggcg tcgagctgtc tgtcgctact acaataatga cctgctccga
     4261 aaccggttca tcggcctcca gttcttcttc aaaacaggtt ctgtgatctg cttcgcctta
     4321 gttttggctg tcctgaggca gcaggacaaa gaggcaagga ccaaagagag cagatccagc
     4381 cctgccgtag agcagcaatt gctagtgtcg gggccaggga agaagccaga ggattcccga
     4441 gtgggatccg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     4501 gatgacgatg acaagtagcg gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg
     4561 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg
     4621 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4681 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4741 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     4801 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     4861 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     4921 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     4981 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     5041 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag
     5101 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag
     5161 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg
     5221 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac
     5281 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc
     5341 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac
     5401 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc
     5461 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc
     5521 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc
     5581 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc
     5641 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag
     5701 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc
     5761 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga
     5821 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
     5881 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5941 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6001 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6061 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6121 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6181 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6241 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6301 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6361 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6421 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6481 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6541 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6601 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6661 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6721 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6781 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6841 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6901 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6961 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7021 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7081 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7141 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7201 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7261 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7321 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7381 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7441 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7561 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7681 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7741 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct
     7801 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7861 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7921 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7981 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8041 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8821 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8881 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8941 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9001 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9061 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9121 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9181 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9241 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9301 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9361 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9421 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9481 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9541 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9601 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9661 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9721 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9781 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9841 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9901 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     9961 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10021 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    10081 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    10141 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10201 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
    10261 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
    10321 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
    10381 gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
