LOCUS       V036182                10783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036182
VERSION     V036182
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4809
                     /label="SLC39A10(NM_001127257)"
                     /note="SLC39A10(NM_001127257)"
                     /gene="SLC39A10"
     CDS             4816..4881
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4925..5136
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5149..5417
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5446..5455
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5476..6138
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6208..6261
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6262..6861
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6862..7450
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7524..7757
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7829..7950
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7990..8125
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8158..8174)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8182..8198
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8206..8236)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8251..8272
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8560..9148)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9319..10179)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10180..10284)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10758..10774
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga aggtacatat gcacacaaaa
     2341 ttttgcctca tttgtttgct gacatttatt tttcatcatt gcaaccattg ccatgaagaa
     2401 catgaccatg gccctgaagc gcttcacaga cagcatcgtg gaatgacaga attggagcca
     2461 agcaaatttt caaagcaagc tgctgaaaat gaaaaaaaat actatattga aaaacttttt
     2521 gagcgttatg gtgaaaatgg aagattatcc ttttttggtt tggagaaact tttaacaaac
     2581 ttgggccttg gagagagaaa agtagttgag attaatcatg aggatcttgg ccacgatcat
     2641 gtttctcatt tagatatttt ggcagttcaa gagggaaagc attttcactc acataaccac
     2701 cagcattccc ataatcattt aaattcagaa aatcaaactg tgaccagtgt atccacaaaa
     2761 agaaaccata aatgtgatcc agagaaagag acagttgaag tgtctgtaaa atctgatgat
     2821 aaacatatgc atgaccataa tcaccgccta cgtcatcacc atcgtttgca tcatcatctt
     2881 gatcataaca acactcacca ttttcataat gattccatta ctcccagtga gcgtggggag
     2941 cctagcaatg aaccttcaac agagaccaat aaaacccagg aacaatctga tgttaaacta
     3001 ccgaaaggaa agaggaagaa aaaagggagg aaaagtaatg aaaattctga ggttattaca
     3061 ccaggttttc cccctaacca tgatcagggt gaacagtatg agcataatcg ggtccacaaa
     3121 cctgatcgtg tacataaccc aggtcattct catgtacatc ttccagaacg taatggtcat
     3181 gatcctggtc gtggacacca agatcttgat cctgataatg aaggtgaact tcgacatact
     3241 agaaagagag aagcaccaca tgttaaaaat aatgcaataa tttctttgag aaaagatcta
     3301 aatgaagatg accatcatca tgaatgtttg aacgtcactc agttattaaa atactatggt
     3361 catggtgcca actctcccat ctcaactgat ttatttacat acctttgccc tgcattgtta
     3421 tatcaaatcg acagcagact ttgtattgag cattttgaca aacttttagt tgaagatata
     3481 aataaggata aaaacctggt tcctgaagat gaggcaaata taggggcatc agcctggatt
     3541 tgtggtatca tttctatcac tgtcattagc ctgctttcct tgctaggcgt gatcttggtt
     3601 cctatcatta accaaggatg cttcaaattc cttcttacat tccttgttgc attagctgta
     3661 ggaacaatga gtggagacgc ccttcttcat ctactgcccc attctcaggg tggacatgat
     3721 cacagtcacc aacatgcaca tgggcatgga cattctcatg gacatgaatc taacaagttt
     3781 ttggaagaat atgatgctgt attgaaagga cttgttgctc taggaggcat ttacttgcta
     3841 tttatcattg aacactgcat tagaatgttt aagcactaca aacaacaaag aggaaaacag
     3901 aaatggttta tgaaacagaa cacagaagaa tcaactattg gaagaaagct ttcagatcac
     3961 aagttaaaca atacaccaga ttctgactgg cttcaactca agcctcttgc cggaactgat
     4021 gactcggttg tttctgaaga tcgacttaat gaaactgaac tgacagattt agaaggccaa
     4081 caagaatccc ctcctaaaaa ttacctttgt atagaagagg agaaaatcat agaccattct
     4141 cacagtgatg gattacatac cattcatgag catgatctcc atgctgctgc acataaccac
     4201 cacggcgaga acaaaactgt gctgaggaag cataatcacc agtggcacca caagcattct
     4261 catcattccc atggcccctg tcattctgga tccgatctga aagaaacagg aatagctaat
     4321 atagcctgga tggtgatcat gggggatggc atccacaact tcagtgatgg gctcgcaatt
     4381 ggtgcagctt tcagtgctgg attgacagga ggaatcagta cttctatagc cgtcttctgt
     4441 catgaactgc cacatgaatt aggagatttt gcagttcttc ttaaagcagg catgactgta
     4501 aagcaagcaa ttgtatacaa cctcctctct gccatgatgg cttacatagg catgctcata
     4561 ggcacagctg ttggtcagta tgccaataac atcacacttt ggatctttgc agtcactgca
     4621 ggcatgttcc tctatgtagc cttggtggat atgcttccag aaatgttgca tggtgatggt
     4681 gacaatgaag aacatggctt ttgtcctgtg gggcaattca tccttcagaa tttaggattg
     4741 ctctttggat ttgccattat gctggtgatt gccctctatg aagataaaat tgtgtttgac
     4801 atccagtttg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     4861 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     4921 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4981 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5041 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5101 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5161 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5221 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5281 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5341 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5401 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5461 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     5521 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     5581 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     5641 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     5701 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     5761 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     5821 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     5881 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     5941 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6001 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6061 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6121 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6181 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6241 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6301 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6361 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6421 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6481 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6541 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6601 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6661 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6721 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6781 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     6841 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     6901 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6961 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7021 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7081 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7141 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7201 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7261 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7321 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7381 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7441 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7501 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct
     7561 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7741 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7801 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7861 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7921 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7981 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8041 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8101 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc
     8161 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8221 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8281 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8341 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9001 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
    10321 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
    10381 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
    10441 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    10501 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    10561 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
    10621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10741 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
