LOCUS       V044140                11380 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044140
VERSION     V044140
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5406
                     /label="SLC12A3(NM_000339)"
                     /note="SLC12A3(NM_000339)"
                     /gene="SLC12A3"
     CDS             5413..5478
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5522..5733
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5746..6014
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6043..6052
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6073..6735
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6805..6858
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6859..7458
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7459..8047
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8121..8354
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8426..8547
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8587..8722
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8755..8771)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8779..8795
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8803..8833)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8848..8869
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9157..9745)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9916..10776)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10777..10881)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11355..11371
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cagaactgcc cacaacagag
     2341 acgcctgggg acgccacttt gtgcagcggg cgcttcacca tcagcacact gctgagcagt
     2401 gatgagccct ctccaccagc tgcctatgac agcagccacc ccagccacct gacccacagc
     2461 agcaccttct gcatgcgcac ctttggctac aacacgatcg atgtggtgcc cacatatgag
     2521 cactatgcca acagcaccca gcctggtgag ccccggaagg tccggcccac actggctgac
     2581 ctgcactcct tcctcaagca ggaaggcaga cacctgcatg ccctggcctt tgacagccgg
     2641 cccagccacg agatgactga tgggctggtg gagggcgagg caggcaccag cagcgagaag
     2701 aaccccgagg agccagtgcg cttcggctgg gtcaaggggg tgatgattcg ttgcatgctc
     2761 aacatttggg gcgtgatcct ctacctgcgg ctgccctgga ttacggccca ggcaggcatc
     2821 gtcctgacct ggatcatcat cctgctgtcg gtcacggtga cctccatcac aggcctctcc
     2881 atctcagcca tctccaccaa tggcaaggtc aagtcaggtg gcacctactt cctcatctcc
     2941 cggagtctgg gcccagagct tgggggctcc atcggcctca ttttcgcttt cgccaatgcc
     3001 gtgggtgtgg ccatgcacac ggtgggcttt gcagagaccg tgcgggacct gctccaggag
     3061 tatggggcac ccatcgtgga ccccattaac gacatccgca tcattgccgt ggtctcggtc
     3121 actgtgctgc tggccatctc cctggctggc atggagtggg agtccaaggc ccaggtgctg
     3181 ttcttccttg tcatcatggt ctcctttgcc aactatttag tggggacgct gatcccccca
     3241 tctgaggaca aggcctccaa aggcttcttc agctaccggg cggacatttt tgtccagaac
     3301 ttggtgcctg actggcgggg tccagatggc accttcttcg gaatgttctc catcttcttc
     3361 ccctcggcca caggcatcct ggcaggggcc aacatatctg gtgacctcaa ggaccctgct
     3421 atagccatcc ccaaggggac cctcatggcc attttctgga cgaccatttc ctacctggcc
     3481 atctcagcca ccattggctc ctgcgtggtg cgtgatgcct ctggggtcct gaatgacaca
     3541 gtgacccctg gctggggtgc ctgcgagggg ctggcctgca gctatggctg gaacttcacc
     3601 gagtgcaccc agcagcacag ctgccactac ggcctcatca actattacca gaccatgagc
     3661 atggtgtcag gcttcgcgcc cctgatcacg gctggcatct tcggggccac cctctcctct
     3721 gccctggcct gccttgtctc tgctgccaaa gtcttccagt gcctttgcga ggaccagctg
     3781 tacccactga tcggcttctt cggcaaaggc tatggcaaga acaaggagcc cgtgcgtggc
     3841 tacctgctgg cctacgccat cgctgtggcc ttcatcatca tcgctgagct caacaccata
     3901 gcccccatca tttccaactt cttcctctgc tcctatgccc tcatcaactt cagctgcttc
     3961 cacgcctcca tcaccaactc gcctgggtgg agaccttcat tccaatacta caacaagtgg
     4021 gcggcgctgt ttggggctat catctccgtg gtcatcatgt tcctcctcac ctggtgggcg
     4081 gccctcatcg ccattggcgt ggtgctcttc ctcctgctct atgtcatcta caagaagcca
     4141 gaggtaaatt ggggctcctc ggtacaggct ggctcctaca acctggccct cagctactcg
     4201 gtgggcctca atgaggtgga agaccacatc aagaactacc gcccccagtg cctggtgctc
     4261 acggggcccc ccaacttccg cccggccctg gtggactttg tgggcacctt cacccggaac
     4321 ctcagcctga tgatctgtgg ccacgtgctc atcggacccc acaagcagag gatgcctgag
     4381 ctccagctca tcgccaacgg gcacaccaag tggctgaaca agaggaagat caaggccttc
     4441 tactcggatg tcattgccga ggacctccgc agaggcgtcc agatcctcat gcaggccgca
     4501 ggtctcggga gaatgaagcc caacattctg gtggttgggt tcaagaagaa ctggcagtcg
     4561 gctcacccgg ccacagtgga agactacatt ggcatcctcc atgatgcctt tgatttcaac
     4621 tatggcgtgt gtgtcatgag gatgcgggag ggactcaacg tgtccaagat gatgcaggcg
     4681 cacattaacc ccgtgtttga cccagcggag gacgggaagg aagccagcgc cagaggtgcc
     4741 aggccatcag tctctggcgc tttggacccc aaggccctgg tgaaggagga gcaggccacc
     4801 accatcttcc agtcggagca gggcaagaag accatagaca tctactggct ctttgacgat
     4861 ggaggcctca ccctcctcat tccctatctc cttggccgca agaggaggtg gagcaaatgc
     4921 aagatccgtg tgttcgtagg cggccagatt aacaggatgg accaggagag aaaggcgatc
     4981 atttctctgc tgagcaagtt ccgactggga ttccatgaag tccacatcct ccctgacatc
     5041 aaccagaacc ctcgggctga gcacaccaag aggtttgagg acatgattgc acccttccgt
     5101 ctgaatgatg gcttcaagga tgaggccact gtcaacgaga tgcggcggga ctgcccctgg
     5161 aagatctcag atgaggagat tacgaagaac agagtcaagt cccttcggca ggtgaggctg
     5221 aatgagattg tgctggatta ctcccgagac gctgctctca tcgtcatcac tttgcccata
     5281 gggaggaagg ggaagtgccc cagctcgctg tacatggcct ggctggagac cctgtcccag
     5341 gacctcagac ctccagtcat cctgatccga ggaaaccagg aaaacgtgct caccttttac
     5401 tgccagggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5461 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5521 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5581 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5641 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5701 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5761 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5821 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5881 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     5941 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6001 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6061 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6121 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6181 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6241 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6301 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6361 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6421 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6481 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6541 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6601 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6661 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6721 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6781 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6841 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6901 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6961 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7021 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7081 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7141 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7201 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7261 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7321 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7381 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7441 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7501 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7561 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7621 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7681 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7741 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7801 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7861 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7921 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7981 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8041 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8101 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8161 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8221 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8281 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8341 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8401 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8521 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8641 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8701 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8761 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8821 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8941 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9781 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9841 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9901 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9961 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10021 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10081 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10141 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10201 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10261 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10321 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10381 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10441 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10501 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10561 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10621 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10681 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10741 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10801 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10861 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10921 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10981 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11041 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11101 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11161 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11221 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11281 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11341 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
